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Brain Edema XV pp 45–48Cite as

Evaluation of the Stage of Hemorrhage Using Optical Diffuse Reflectance Spectroscopy: An In Vivo Study

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Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 118))

Abstract

Intracerebral hemorrhage (ICH) is a common and often fatal subtype of stroke. Estimation of the stage of hemorrhage allows clinicians to know when the hemorrhage occurred, even in unconscious patients, enabling decisions to be made about the optimal management and treatment strategy. After ICH, oxidative denaturation of the hemoglobin progresses, and deoxyhemoglobin is gradually converted to methemoglobin. MRI has been used to estimate the stage of hemorrhage by evaluating the status of hemoglobin. However, there is currently no bedside device that can be used for the measurement of hemoglobin derivatives in patients with hematomas. The aim of the present study was to investigate the validity of using optical diffuse reflectance spectroscopy (ODRS) for bedside evaluation of the stage of hemorrhage. An ICH model was generated in adult Sprague–Dawley male rats by stereotactically injecting 50 μl of autologous blood into the right caudate nucleus. To analyze the hemoglobin derivatives in the hematomas, ODRS measurement was performed for the rats in vivo. In all rats, we found increased absorption at around 630 nm, which indicated the formation of methemoglobin. In conclusion, the results of the present study suggest that ODRS allows clinicians to more easily evaluate the stage of hemorrhage at the patient’s bedside.

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Acknowledgements

This study was supported by a research grant from The General Insurance Association of Japan.

Conflict of InterestWe declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Satoru Takeuchi, Hiroshi Nawashiro, Kimihiro Nagatani, Hiroaki Kobayashi, Naoki Otani MD, Hideo Osada, Kojiro Wada & Katsuji Shima

  2. Division of Biomedical Information Sciences, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Satoko Kawauchi & Shunichi Sato

Authors
  1. Satoru Takeuchi
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  2. Satoko Kawauchi
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  3. Shunichi Sato
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  4. Hiroshi Nawashiro
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  5. Kimihiro Nagatani
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  6. Hiroaki Kobayashi
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  7. Naoki Otani MD
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  8. Hideo Osada
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  9. Kojiro Wada
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  10. Katsuji Shima
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Correspondence to Satoru Takeuchi .

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Editors and Affiliations

  1. School of Medicine, Dept. Neurological Surgery, Nihon University, Oyaguchikami-machi 30-1, Tokyo, 173-8610, Japan

    Yoichi Katayama

  2. School of Medicine, Departments of Neurological Surgery and, Nihon University, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan

    Takeshi Maeda

  3. , Department of Clinical Laboratory, Namegata District General Hospital, 98-8 Inouefujii, Namegata 98-8, Ibaraki, 311-3516, Japan

    Toshihiko Kuroiwa

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Takeuchi, S. et al. (2013). Evaluation of the Stage of Hemorrhage Using Optical Diffuse Reflectance Spectroscopy: An In Vivo Study. In: Katayama, Y., Maeda, T., Kuroiwa, T. (eds) Brain Edema XV. Acta Neurochirurgica Supplement, vol 118. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1434-6_7

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  • DOI: https://doi.org/10.1007/978-3-7091-1434-6_7

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1433-9

  • Online ISBN: 978-3-7091-1434-6

  • eBook Packages: MedicineMedicine (R0)

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