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Homocysteine-Thiolactone

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Abstract

Hcy-thiolactone was discovered by serendipity in 1934 as a by-product of an early assay for the quantification of methionine in proteins [187]. The assay involves boiling with hydriodic acid (128 °C, 3 h). This causes demethylation of methionine with the formation of methyl iodide, whose quantitative recovery (99.5 ± 2.0 %) by absorption in alcoholic solution of silver nitrate was the basis of the methionine assay. The residue from methionine that remains in the acid has been identified by elemental analysis as Hcy-thiolactone. The recovery of Hcy-thiolactone from methionine digestion is also quantitative (97.0 ± 2.1 %) [187].

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  1. Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School International Center for Public Health, Newark, NJ, USA

    Hieronim Jakubowski

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Jakubowski, H. (2013). Homocysteine-Thiolactone. In: Homocysteine in Protein Structure/Function and Human Disease. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1410-0_3

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