Skip to main content
SpringerLink
Log in

Spatially resolved imaging methods to probe metals in the brain: from subcellular to organ level

  • Chapter
  • First Online:
Metal Ions in Neurological Systems

Abstract

Very little is known about the subcellular distribution of metal ions in cells. Some metals such as zinc, copper, and iron are essential and play an important role in the cell metabolism. Dysfunctions in this delicate housekeeping may be at the origin of major diseases. There is also a prevalent use of metals in a wide range of diagnostic agents and drugs for the diagnosis or treatment of a variety of disorders. This is becoming more and more of a concern in the field of nanomedicine with the increasing development and use of nanoparticles, which are suspected of causing adverse effects on cells and organ tissues and particularly the brain. Various analytical methods are developing into well-suited sub-micrometer analytical tools for addressing new problems when studying the role of metals in the brain. In the present review, we describe the possibilities and some current applications offered by three major techniques that cover imaging of metals from subcellular to organ level, namely synchrotron-based X-ray microspectroscopy, laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) and, less thoroughly addressed, the capabilities of magnetic resonance imaging (MRI) for in vivo imaging of metals in the brain.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Zastrow ML, Peacock AFA, Stuckey JA, Pecoraro VL (2011) Nat Chem

    Google Scholar 

  2. Schmitt C, Strazielle N, Richaud P, Bouron A, Ghersi-Egea JF (2011) J Neurochem 117:747

    PubMed  CAS  Google Scholar 

  3. Sensi SL, Paoletti P, Koh JY, Aizenman E, Bush AI, Hershfinkel M (2011) J Neurosci 31:16076

    PubMed  CAS  Google Scholar 

  4. Sensi SL, Paoletti P, Bush AI, Sekler I (2009) Nat Rev Neurosci 10:780

    PubMed  CAS  Google Scholar 

  5. Zecca L, Youdim MB, Riederer P, Connor JR, Crichton RR (2004) Nat Rev Neurosci 5:863

    PubMed  CAS  Google Scholar 

  6. Sloot WN, Gramsbergen JB (1994) Brain Res 657:124

    PubMed  CAS  Google Scholar 

  7. Zheng W, Monnot AD (2011) Pharmacol Ther

    Google Scholar 

  8. Zhang S, Rocourt C, Cheng WH (2010) Mech Ageing Dev 131:253

    PubMed  CAS  Google Scholar 

  9. Tarohda T, Yamamoto M, Amamo R (2004) Anal Bioanal Chem 380:240

    PubMed  CAS  Google Scholar 

  10. Tarohda T, Ishida Y, Kawai K, Yamamoto M, Amano R (2005) Anal Bioanal Chem 383:224

    PubMed  CAS  Google Scholar 

  11. Pushie MJ, Pickering IJ, Martin GR, Tsutsui S, Jirik FR, George GN (2011) Metallomics 3:206

    PubMed  CAS  Google Scholar 

  12. Pearse AGE (1985) Histochemistry, theoretical and applied. Churchill livingston, Edinburgh

    Google Scholar 

  13. Danscher G (1991) Prog Histochem Cytochem 23:273

    PubMed  CAS  Google Scholar 

  14. Lopez-Garcia C, Varea E, Palop JJ, Nacher J, Ramirez C, Ponsoda X, Molowny A (2002) Microsc Res Tech 56:318

    PubMed  CAS  Google Scholar 

  15. Que EL, Domaille DW, Chang CJ (2008) Chem Rev 108:1517

    PubMed  CAS  Google Scholar 

  16. Domaille DW, Que EL, Chang CJ (2008) Nat Chem Biol 168

    Google Scholar 

  17. Leapman RD (2004) Curr Opin Neurobiol 14:591

    PubMed  CAS  Google Scholar 

  18. Barberet P, Daudin L, Gordillo N, Sorieul S, Simon M, Seznec H, Idarraga I, Incerti S, Balana A, Moretto Ph (2011) Nucl Instrum Methods Phys Res B 269:2163

    CAS  Google Scholar 

  19. Bohic S, Simionovici A, Ortega R, Heymann D, Schroer C, Snigirev A (2001) Nucl Instrum Methods Phys Res B 181:728

    CAS  Google Scholar 

  20. Ortega R, Cloetens P, Deves G, Carmona A, Bohic S (2007) PLoS One 2:e925

    PubMed  Google Scholar 

  21. Bohic S, Cotte M, Salome M, Fayard B, Kuehbacher M, Cloetens P, Martinez-Criado G, Tucoulou R, Susini J (2011) J Struct Biol

    Google Scholar 

  22. Ortega R, Deves G, Carmona A (2009) J R Soc Interface 6(Suppl 5):S649

    PubMed  CAS  Google Scholar 

  23. Korbas M, Macdonald TC, Pickering IJ, George GN, Krone PH (2011) ACS Chem Biol

    Google Scholar 

  24. Bacquart T, Deves G, Carmona A, Tucoulou R, Bohic S, Ortega R (2007) Anal Chem 79:7353

    PubMed  CAS  Google Scholar 

  25. Carter EA et al (2010) Mol Biosyst 6:1316

    PubMed  CAS  Google Scholar 

  26. Bohic S, Murphy K, Paulus W, Cloetens P, Salome M, Susini J, Double K (2008) Anal Chem 80:9557

    PubMed  CAS  Google Scholar 

  27. Al-Amoudi A, Norlen LP, Dubochet J (2004) J Struct Biol 148:131

    PubMed  CAS  Google Scholar 

  28. Matsuyama S (2010) X-ray Spectrom 39:260

    CAS  Google Scholar 

  29. Ortega R et al (2009) Toxicol Lett 188:26

    PubMed  CAS  Google Scholar 

  30. James SA et al (2011) Anal Bioanal Chem 401:853

    PubMed  CAS  Google Scholar 

  31. Hackett MJ et al (2011) Analyst 136:2941

    PubMed  CAS  Google Scholar 

  32. Chwiej J, Szczerbowska-Boruchowska M, Lankosz M, Wojcik S, Falkenberg G, Stegowski Z, Setkowicz Z (2005) Spectrochim Acta Part B At Spectrosc 60:1531

    Google Scholar 

  33. Schrag M, Dickson A, Jiffry A, Kirsch D, Vinters HV, Kirsch WM (2010) BioMetals 23:1123

    Google Scholar 

  34. Gibon J, Tu P, Bohic S, Richaud P, Arnaud J, Zhu M, Boulay G, Bouron A (2011) Biochim Biophys Acta 1808:2807

    PubMed  CAS  Google Scholar 

  35. Duong TT, Witting PK, Antao ST, Parry SN, Kennerson M, Lai B, Vogt S, Lay PA, Harris HH (2009) J Neurochem 108:1143

    PubMed  CAS  Google Scholar 

  36. Dodani SC, Domaille DW, Nam CI, Miller EW, Finney LA, Vogt S, Chang CJ (2011) Proc Natl Acad Sci USA 108:5980

    PubMed  CAS  Google Scholar 

  37. Ducic T, Quintes S, Nave KA, Susini J, Rak M, Tucoulou R, Alevra M, Guttmann P, Salditt T (2011) J Struct Biol 173:202

    PubMed  CAS  Google Scholar 

  38. Carmona A, Deves G, Roudeau S, Cloetens P, Bohic S, Ortega R (2010) ACS Chem Neurosci 1:194

    PubMed  CAS  Google Scholar 

  39. Miller LM, Wang Q, Telivala TP, Smith RJ, Lanzirotti A, Miklossy J (2006) J Struct Biol 155:30

    PubMed  CAS  Google Scholar 

  40. Leskovjan AC, Lanzirotti A, Miller LM (2009) NeuroImage 47:1215

    PubMed  Google Scholar 

  41. Kühbacher M, Weseloh G, Thomzig A, Bertelsmann H, Falkenberg G, Radtke M, Riesemeier H, Kyriakopoulos A, Beekes M, Behne D (2005) X-ray Spectrom 34:112

    Google Scholar 

  42. Linkous DH, Flinn JM, Koh JY, Lanzirotti A, Bertsch PM, Jones BF, Giblin LJ, Frederickson CJ (2008) J Histochem Cytochem 56:3

    PubMed  CAS  Google Scholar 

  43. Gh Popescu BF, Nichol H (2011) CNS Neurosci Ther 17:256

    Google Scholar 

  44. Popescu BF et al (2009) Phys Med Biol 54:651

    PubMed  Google Scholar 

  45. Popescu BF, Robinson CA, Chapman LD, Nichol H (2009) Cerebellum 8:340

    PubMed  Google Scholar 

  46. Kastyak MZ, Szczerbowska-Boruchowska M, Adamek D, Tomik B, Lankosz M, Gough KM (2010) Neuroscience 166:1119

    PubMed  CAS  Google Scholar 

  47. Tomik B et al (2006) Neurochem Res 31:321

    PubMed  CAS  Google Scholar 

  48. Szczerbowska-Boruchowska M, Lankosz M, Adamek D (2011) J Biol Inorg Chem 16:1217

    PubMed  CAS  Google Scholar 

  49. Penner-Hahn JE (2005) Coord Chem Rev 249:161

    CAS  Google Scholar 

  50. Gunter KK, Aschner M, Miller LM, Eliseev R, Salter J, Anderson K, Gunter TE (2006) Neurobiol Aging 27:1816

    PubMed  CAS  Google Scholar 

  51. Gunter KK, Aschner M, Miller LM, Eliseev R, Salter J, Anderson K, Hammond S, Gunter TE (2005) Free Radic Biol Med 39:164

    PubMed  CAS  Google Scholar 

  52. Gunter TE, Gavin CE, Aschner M, Gunter KK (2006) Neurotoxicology 27:765

    PubMed  CAS  Google Scholar 

  53. Lobinski R, Moulin C, Ortega R (2006) Biochimie 88:1591

    PubMed  CAS  Google Scholar 

  54. Popescu BF, Pickering IJ, George GN, Nichol H (2007) J Inorg Biochem 101:957

    PubMed  CAS  Google Scholar 

  55. Yoshida S, Ide-Ektessabi A, Fujisawa S (2003) Struct Chem 14:85

    CAS  Google Scholar 

  56. Ide-Ektessabi A, Rabionet M (2005) Anal Sci 21:885

    PubMed  CAS  Google Scholar 

  57. Chwiej J, Adamek D, Szczerbowska-Boruchowska M, Krygowska-Wajs A, Wojcik S, Falkenberg G, Manka A, Lankosz M (2007) J Biol Inorg Chem 12:204

    PubMed  CAS  Google Scholar 

  58. Chwiej J, Adamek D, Szczerbowska-Boruchowska M, Krygowska-Wajs A, Bohic S, Lankosz M (2008) J Trace Elem Med Biol 22:183

    PubMed  CAS  Google Scholar 

  59. Chang LW, Verity MA (1995) Mercury neurotoxicity: effects and mechanisms. In: Chang LW, Dyers RS (eds) Handbook of neurotoxicology. Dekker, New York, p 31

    Google Scholar 

  60. Ibrahim D, Froberg B, Wolf A, Rusyniak DE (2006) Clin Lab Med 26:67

    PubMed  Google Scholar 

  61. Zoeger N, Streli C, Wobrauschek P, Jokubonis C, Pepponi G, Roschger P, Bohic S, Osterode W (2005) Powder Diffraction 20:158

    CAS  Google Scholar 

  62. Korbas M, O’Donoghue JL, Watson GE, Pickering IJ, Singh SP, Myers GJ, Clarkson TW, George GN (2010) ACS Chem Neurosci 1:810

    PubMed  CAS  Google Scholar 

  63. Gray AL (1985) Analyst 110:551

    CAS  Google Scholar 

  64. Mokgalaka N, Gardea-Torresdey J (2006) Appl Spectrosc Rev 41:131

    CAS  Google Scholar 

  65. Becker JS, Zoriy M, Matusch A, Wu B, Salber D, Palm C (2010) Mass Spectrom Rev 29:156

    PubMed  CAS  Google Scholar 

  66. Becker JS, Matusch A, Palm C, Salber D, Morton KA (2010) Metallomics 2:104

    PubMed  Google Scholar 

  67. Smeal J, Wilkins CL (2011) Appl Spectrosc Rev 46:425

    Google Scholar 

  68. Gunther D, Heinrich CA (1999) J Anal At Spectrom 14:1363

    CAS  Google Scholar 

  69. Potter D (2008) J Anal At Spectrom 23:690

    CAS  Google Scholar 

  70. Durrant SF (1999) J Anal At Spectrom 14:1385

    CAS  Google Scholar 

  71. Durrant SF, Ward NI (2005) J Anal At Spectrom 20:821

    CAS  Google Scholar 

  72. Caprioli RM, Farmer TB, Gile J (1997) Anal Chem 69:4751

    PubMed  CAS  Google Scholar 

  73. Hare D, Burger F, Austin C, Fryer F, Grimm R, Reedy B, Scolyer RA, Thompson JF, Doble P (2009) Analyst 134:450

    PubMed  CAS  Google Scholar 

  74. Hare D, Tolmachev S, James A, Bishop D, Austin C, Fryer F, Doble P (2010) Anal Chem 82:3176

    PubMed  CAS  Google Scholar 

  75. Feldmann J, Kindness A, Ek P (2002) J Anal At Spectrom 17:813

    CAS  Google Scholar 

  76. Kindness A, Sekaran CN, Feldmann J (2003) Clin Chem 49:1916

    PubMed  CAS  Google Scholar 

  77. Austin C, Fryer F, Lear J, Bishop D, Hare D, Rawling T, Kirkup L, McDonagh A, Doble P (2011) J Anal At Spectrom 26:1494

    CAS  Google Scholar 

  78. Zoriy MV, Dehnhardt M, Matusch A, Becker J (2008) Spectrochim Acta Part B At Spectrosc 63:375

    Google Scholar 

  79. Becker JS, Dobrowolska J, Zoriy M, Matusch A (2008) Rapid Commun Mass Spectrom 22:2768

    PubMed  CAS  Google Scholar 

  80. Becker JS, Zoriy MV, Pickhardt C, Palomero-Gallagher N, Zilles K (2005) Anal Chem 77:3208

    PubMed  CAS  Google Scholar 

  81. Zoriy MV, Becker JS (2007) Int J Mass Spectrom 264:175

    CAS  Google Scholar 

  82. Dehnhardt M, Zoriy MV, Khan Z, Reifenberger G, Ekstrom TJ, Sabine Becker J, Zilles K, Bauer A (2008) J Trace Elem Med Biol 22:17

    PubMed  CAS  Google Scholar 

  83. Becker JS, Zoriy MV, Pickhardta C, Zilles K (2005) J Anal At Spectrom 20:912

    CAS  Google Scholar 

  84. Jackson B, Harper S, Smith L, Flinn J (2006) Anal Bioanal Chem 384:951

    PubMed  CAS  Google Scholar 

  85. Hare D, Reedy B, Grimm R, Wilkins S, Woltakis I, George JL, Cherny RA, Bush AI, Finkelstein DI, Doble P (2009) Metallomics 1:53

    CAS  Google Scholar 

  86. Hutchinson RW, Cox AG, McLeod CW, Marshall PS, Harper A, Dawson EL, Howlett DR (2005) Anal Biochem 346:225

    PubMed  CAS  Google Scholar 

  87. Lear J, Hare D, Adlard P, Finkelstein D, Doble P (2012) J Anal At Spectrom 27:159

    Google Scholar 

  88. Stockle R, Setz P, Deckert V, Lippert T, Wokaun A, Zenobi R (2001) Anal Chem 73:1399

    PubMed  CAS  Google Scholar 

  89. Giesen C, Waentig L, Mairinger T, Drescher D, Kneipp J, Roos PH, Panneab U, Jakubowski N (2011) J Anal At Spectrom 26:2160

    Google Scholar 

  90. Sela H, Karpas Z, Zoriy M, Pickhardt C, Becker JS (2007) Int J Mass Spectrom 261:199

    CAS  Google Scholar 

  91. Pugh JAT, Cox AG, McLeod CW, Bunch J, Whitby B, Gordon B, Kalber T, White E (2011) J Anal At Spectrom 26:1667

    CAS  Google Scholar 

  92. Bellis DJ, Santamaria-Fernandez R (2010) J Anal At Spectrom 25:957

    Google Scholar 

  93. Corot C, Robert P, Idee JM, Port M (2006) Adv Drug Deliv Rev 58:1471

    PubMed  CAS  Google Scholar 

  94. Barbier EL, Lamalle L, Decorps M (2001) J Magn Reson Imaging 13:496

    PubMed  CAS  Google Scholar 

  95. Saleh A, Schroeter M, Jonkmanns C, Hartung HP, Modder U, Jander S (2004) Brain 127:1670

    PubMed  Google Scholar 

  96. Desestret V, Brisset JC, Moucharrafie S, Devillard E, Nataf S, Honnorat J, Nighoghossian N, Berthezene Y, Wiart M (2009) Stroke 40:1834

    PubMed  Google Scholar 

  97. Weinstein JS, Varallyay CG, Dosa E, Gahramanov S, Hamilton B, Rooney WD, Muldoon LL, Neuwelt EA (2010) J Cereb Blood Flow Metab 30:15

    PubMed  CAS  Google Scholar 

  98. Valable S, Barbier EL, Bernaudin M, Roussel S, Segebarth C, Petit E, Remy C (2007) NeuroImage 37(Suppl 1):S47

    PubMed  Google Scholar 

  99. Wilhelm C, Gazeau F (2008) Biomaterials 29:3161

    PubMed  CAS  Google Scholar 

  100. Frank JA, Miller BR, Arbab AS, Zywicke HA, Jordan EK, Lewis BK, Bryant LH Jr, Bulte JW (2003) Radiology 228:480

    PubMed  Google Scholar 

  101. Arbab AS, Bashaw LA, Miller BR, Jordan EK, Bulte JW, Frank JA (2003) Transplantation 76:1123

    PubMed  CAS  Google Scholar 

  102. Walczak P, Kedziorek DA, Gilad AA, Lin S, Bulte JW (2005) Magn Reson Med 54:769

    PubMed  CAS  Google Scholar 

  103. Smirnov P, Poirier-Quinot M, Wilhelm C, Lavergne E, Ginefri JC, Combadiere B, Clement O, Darrasse L, Gazeau F (2008) Magn Reson Med 60:1292

    PubMed  Google Scholar 

  104. Shapiro EM, Sharer K, Skrtic S, Koretsky AP (2006) Magn Reson Med 55:242

    PubMed  Google Scholar 

  105. Lux F et al (2011) Angew Chem Int Ed Engl 50:12299

    PubMed  CAS  Google Scholar 

  106. Guenoun J, Koning GA, Doeswijk G, Bosman L, Wielopolski PA, Krestin GP, Bernsen MR (2011) Cell Transplant

    Google Scholar 

  107. Lin YJ, Koretsky AP (1997) Magn Reson Med 38:378

    PubMed  CAS  Google Scholar 

  108. Pautler RG, Silva AC, Koretsky AP (1998) Magn Reson Med 40:740

    PubMed  CAS  Google Scholar 

  109. Watanabe T, Frahm J, Michaelis T (2004) NMR Biomed 17:554

    PubMed  CAS  Google Scholar 

  110. Lindsey JD, Scadeng M, Dubowitz DJ, Crowston JG, Weinreb RN (2007) NeuroImage 34:1619

    PubMed  Google Scholar 

  111. Jackson SJ, Hussey R, Jansen MA, Merrifield GD, Marshall I, Maclullich A, Yau JL, Bast T. Behav Brain Res

    Google Scholar 

  112. Bilgic B, Pfefferbaum A, Rohlfing T, Sullivan EV, Adalsteinsson E (2011) Neuroimage

    Google Scholar 

  113. Fukunaga M et al (2010) Proc Natl Acad Sci USA 107:3834

    PubMed  CAS  Google Scholar 

  114. Murphy GE, Narayan K, Lowekamp BC, Hartnell LM, Heymann JA, Fu J, Subramaniam S (2011) J Struct Biol 176:268

    PubMed  Google Scholar 

  115. Guerquin-Kern JL, Wu TD, Quintana C, Croisy A (2005) Biochim Biophys Acta 1724:228

    PubMed  CAS  Google Scholar 

  116. Quintana C, Wu TD, Delatour B, Dhenain M, Guerquin-Kern JL, Croisy A (2007) Microsc Res Tech 70:281

    PubMed  CAS  Google Scholar 

  117. Antharam V, Collingwood JF, Bullivant JP, Davidson MR, Chandra S, Mikhaylova A, Finnegan ME, Batich C, Forder JR, Dobson J (2012) NeuroImage 59:1249

    PubMed  Google Scholar 

  118. Hopp K, Popescu BF, McCrea RP, Harder SL, Robinson CA, Haacke ME, Rajput AH, Rajput A, Nichol H (2010) J Magn Reson Imaging 31:1346

    PubMed  Google Scholar 

  119. Valable S, Barbier EL, Bernaudin M, Roussel S, Segebarth C, Petit E, Remy C (2008) NeuroImage 40:973

    PubMed  Google Scholar 

Download references

Acknowledgements

The European Synchrotron Radiation Facility is acknowledged for provision of synchrotron radiation beam time. This work was supported by the Partenariats Hubert Curien (PHC)-FAST and the Australian Department of Innovation, Industry, Science and Research International Science Linkage FAST programs.

Author information

Authors and Affiliations

  1. Inserm U-836, Grenoble Institut des Neurosciences, Team 6: Rayonnement Synchrotron et Recherches Médicales, ESRF, 6 rue Jules Horowitz, 220, 38043, Grenoble, France

    Sylvain Bohic

  2. European Synchrotron Radiation Facility, ESRF, 220, Grenoble, France

    Sylvain Bohic

  3. Grenoble Institut des Neurosciences, Université Joseph Fourier 1, Grenoble, 38042, France

    Sylvain Bohic

  4. Elemental Bio-imaging Facility, University of Technology, Sydney, Broadway, New South Wales, Australia

    Sylvain Bohic

  5. Elemental Bio-imaging Facility, University of Technology, Sydney, Broadway, NSW, Australia

    Dominic Hare

  6. Inserm, U836, Grenoble Institut des Neurosciences, Grenoble, France

    Alexia Daoust & Emmanuel L. Barbier

  7. Grenoble Institut des Neurosciences, Université Joseph Fourier 1, Grenoble, France

    Alexia Daoust & Emmanuel L. Barbier

  8. European Synchrotron Radiation Facility, ESRF, Grenoble, France

    Peter Cloetens

Authors
  1. Sylvain Bohic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dominic Hare
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexia Daoust
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter Cloetens
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Emmanuel L. Barbier
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sylvain Bohic .

Editor information

Editors and Affiliations

  1. , Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9, Vienna, 1060, Austria

    Wolfgang Linert

  2. Faculty of Chemistry, University of Wroclaw, ul. Fryderyka Joliot Curie 14, Wroclaw, 50-383, Poland

    Henryk Kozlowski

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer-Verlag Wien

About this chapter

Cite this chapter

Bohic, S., Hare, D., Daoust, A., Cloetens, P., Barbier, E.L. (2012). Spatially resolved imaging methods to probe metals in the brain: from subcellular to organ level. In: Linert, W., Kozlowski, H. (eds) Metal Ions in Neurological Systems. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1001-0_18

Download citation

Publish with us

Policies and ethics

Search

Navigation