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Genetic Diversity Among the Microorganisms in Daqu Used for Beidacang Liquor as Revealed by RAPD Analyses

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Book cover Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 333))

Abstract

To assess the genetic diversity among five microorganisms (namely, DSL(CS)-01, DSL(CS)-02, DSL(PDA)-C, DSL(PDA)-E, DSL(PDA)-F), RAPD (random amplified polymorphic DNA) analysis was performed. Seven primers with stable polymorphisms were screened from 64 primers. PCR was carried out with the seven primers. The data indicated that the molecular markers gave different levels of polymorphism. 94 RAPD bands were obtained and out of them 64 were polymorphic bands (68.09 %) and the best primer screened was b8. The primer b8 was considered as the most effective RAPD marker for the five microorganisms above. The dendrogram built on the basis of data from RAPD analysis represented the genetic distances among the five microorganisms. Understanding the genetic variability among the microorganisms opens up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic improvement of the microorganisms.

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References

  1. Ahmad N, Munir I, Khan I et al (2007) PCR-based genetic diversity of rape seed germplasm using RAPD markers. Biotechnology 6(3):334–338

    Article  CAS  Google Scholar 

  2. Chao J, Zhang X (2005) Technical progress and development in liquor-making industry in China. Liquor-Making Sci Technol 5:10–16

    Google Scholar 

  3. Chen Y (2013) Analysis of technical development trend in liquor-making industry. Liquor-Making Sci Technol 3:11–13

    Google Scholar 

  4. Du L, Rao J, Tang C et al (2010) Application of cellar functional microbes in Luzhou flavour liquor production. Liquor Making 37(6):43–44

    Google Scholar 

  5. Du L, Wang Y, Rao J et al (2009) Studies on the culture and application of cellar mud complex functional microorganism. Liquor Making 36(5):35–36

    CAS  Google Scholar 

  6. Guo K (2002) Investigation on value of research and the reasons of microbial biodiversity of Mao-tai liquor. Liquor Making 29(2):36–38

    Google Scholar 

  7. Jiang H (2003) Research and prospect of the brewing microbial diversity of Mao-tai-favour liquor in the ecological environment. Liquor Making 30(4):22–24

    Google Scholar 

  8. Li X (2004) Review of development and trend of Chinese liquor. Liquor Making 1:9–10

    Google Scholar 

  9. Li Z, Wang D, Ma M et al (2010) Comparison of enzymes species and fermentation performance of different liquor starter. Liquor-Making Sci Technol 1:17–22

    Google Scholar 

  10. Nei M (1972) Genetic distance between populations. American. Nature 106:183–292

    Google Scholar 

  11. Wang C, Shi D, Gong G (2008) Microorganisms in Daqu: a starter culture of Chinese Mao-tai-flavour liquor. World J Microbiol Biotechnol 24(10):2183–2190

    Article  Google Scholar 

  12. Wang R, Guan F, Yu H et al (2001) The research for application of dried active pit mud function microbe in production of heavy fragrant Chinese spirits. Liquor Making 28(6):35–36

    Google Scholar 

  13. Wang S, Dong Y, Li T et al (2006) The method of DNA effective and quick means for DNA extraction of taxol producing fungi. J Sci Teach Coll Univ 26(1):60–64

    Google Scholar 

  14. Wang W, Jiang F, Zhao T et al (2014) Technical progress of liquors of the main flavour types. Liquor-Making Sci Technol 3:88–89

    Google Scholar 

  15. Zhuang M, Wang Z (2003) Separation, breeding selection and taxonomic determination of functional bacteria strain B3-1 from high temperature Daqu starter for Mao-tai-flavour liquors. Liquor Making 30(5):26–28

    Google Scholar 

  16. Zeng Z (2002) On the relationship favour components in liquor and quality style. Liquor Making 29(1):8–10

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Foundation of Hei longjiang Educational Committee (No. 12541855).

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Authors and Affiliations

  1. The College of Life Sciences and Engineering, Qiqihar University, No. 42, Wenhua Street, Qiqihar, Heilongjiang, China

    Shi-Wei Wang, Qing-Hui Wang, Li-Ping Zhai, Jun Liu, Zhi-Dan Yu, Mao-Mao Zheng & Fang Wang

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  1. Shi-Wei Wang
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  2. Qing-Hui Wang
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  3. Li-Ping Zhai
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  4. Jun Liu
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  5. Zhi-Dan Yu
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  6. Mao-Mao Zheng
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  7. Fang Wang
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Corresponding author

Correspondence to Shi-Wei Wang .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Wang, SW. et al. (2015). Genetic Diversity Among the Microorganisms in Daqu Used for Beidacang Liquor as Revealed by RAPD Analyses. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_11

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