Abstract
The susceptibility of the frog embryo to infective transmission of the renal adenocarcinoma was shown following inoculation of R. pipiens embryos with subcellular fractions and filtrates from inclusion body tumors (1). The cytoplasmic fractions injected were rich in the herpes-like virus also seen in the source tumor. Induction of renal carcinomas, identical to spontaneous actively growing primary adenocarcinomas occurred in 66% to over 90% of the hosts. Tumors were found in both pronephric and mesonephric kidneys and were palpable prior to or during metamorphosis. While the success of the injections depended upon the embryonic stage, all recipients from the blastula through hatching stage were inducible. Subsequently, Mulcare (2, 3) has found that similar fractions will transmit renal tumors through the embryos of a closely related species Rana palustris.
This research was supported by Grant CA-07849 from the National Cancer Institute, NIH, Bethesda, Md.
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References
Tweedell, K. S.: Induced oncogenesis in developing frog kidney cells. Cancer Res. 27:2042–2052, 1967.
Mulcare, D. J. and Tweedell, K. S.: Renal tumors induced in Kana palustris by tumor implants or subcellular fractions from Kana pipicns. Amer. Zool. 7:176, 1967.
Mulcare, D. J.: Non-specific transmission of the Lucke tumor. In Biology of Amphibian Tumors (M. Mizell, ed.), Springer-Verlag New York Inc., 1969, pp. 240–253.
Rafferty, K. A., Jr.: Kidney tumors of the leopard frog: a review. Cancer Res. 24:169–185, 1964.
Mizell, M. and Zambernard, J.: Viral particles of the leopard frog renal adenocarcinoma: Causative agent or passenger virus? II. A promising model system for the demonstration of a “lysogenic” state in a metazoan tumor. Ann. N.Y. Acad. Sci. 726:146–169, 1965.
Mizell, M., Stackpole, W. and Halpern S.: Herpes-type virus recovery from virus-free frog kidney tumors. Proc. Soc. Exp. Biol. Med. 127:808–814, 1968.
Shumway, W.: Stages in the normal development of Kana pipiens. Anat. Rec. 7 8:139–148, 1940.
Rugh, R.: Ovulation in the frog. II. Follicular rupture to fertilization. J. Exper. Zool. 71:163–193, 1935.
Tweedell, K. and Granoff, A.: Viruses and renal carcinoma of Kana pipiens. V. Effect of frog virus 3 on developing frog embryos and larvae. J. Nat. Cancer Instit. 40:407–410, 1968.
Rafferty, K. A., Jr.: The biology of spontaneous renal carcinoma of the frog. In Renal Neoplasia (J. King, ed.), Little, Brown and Co., Boston, Mass., 1967.
Rafferty, K. A., Jr.: The cultivation of inclusion associated viruses from Lucke tumor frogs. Ann. N.Y. Acad. Sci. 126:3–21, 1965.
Granoff, A. and Tweedell, K. S.: Unpublished Observations.
Granoff, A., Came, P. E., and Rafferty, K. A., Jr.: The isolation and properties of viruses from Kana pipiens: their possible relationship to the renal adenocarcinoma of the leopard frog. Ann. N.Y. Acad. Sci. 126:237–255, 1965.
Lucke, B.: Kidney carcinoma of the leopard frog: a virus tumor. Ann. N.Y. Acad. Sci. 54:1093–1109, 1952.
Rafferty, K. A., Jr.: Spontaneous kidney tumors in the frog: rate of occurrence in isolated adults. Science 242:720–721, 1963.
Roberts, M. E.: Studies on the transmissibility and cytology of the renal carcinoma of Kana pipiens. Cancer Res. 23:1709–1714, 1963.
Di Berardino, M. A., and King, T. J.: Renal adenocarcinomas induced by crowded conditions in laboratory frogs. Cancer Res. 25:1910–1912, 1965.
Rubin, M. L., Ar, D. B., and Nace, G. W.: The virus inhibiting action of a lysozyme present in normal frogs but lacking in tumor frogs and eggs. Amer. Zool. 6:510, 1966.
Mc Kinnell, R. G., and Mc Kinnell, B. K.: Seasonal fluctuation of frog renal adenocarcinoma prevalence in natural populations. Cancer Res. 28:440–444, 1968.
Mc Kinnell, R. G., and Zambernard, J.: Virus particles in renal tumors obtained from spring Kana pipiens of known geographic origin. Cancer Res. 28:684–688, 1968
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Tweedell, K.S. (1969). Simulated Transmission of Renal Tumors in Oocytes and Embryos of Rana pipiens . In: Mizell, M. (eds) Biology of Amphibian Tumors. Recent Results in Cancer Research, vol 1969. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85791-1_20
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