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Adipocytes and Osteoblasts from Human Adipose Tissue Mesenchymal Stem Cells for the Production of Compatible and Safe Biomaterial Crucial in Cosmetic, Reconstructive, and Regenerative Medicine

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Stem Cells in Aesthetic Procedures

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Abstract

It is well established that mesenchymal stem cells (MSCs) with identical features as those from human bone marrow (BM) and human umbilical cord blood (UCB) can be obtained from different sources such as amniotic fluid, amniotic membrane, and adipose tissue. This project has particularly focused on the specific features of MSCs from human adipose tissue (hATMSCs) with the aim of verifying whether adipose tissue may be considered a valid source of multipotent MSCs compared with different sources such as BM or UCB and if their derived cells might keep any transdifferentiation ability. The use of adipose tissue reveals evident advantages: it is easy to obtain, painless, and minimally invasive; secondly, the isolated MSCs have shown a high grade of proliferation and growth rate, multilineage cell line differentiation capacity, and a great plasticity. Therefore, to confirm this premise, the study has been structured in three phases: (1) the isolated MSCs were induced to adipocytes and osteoblasts; (2) the adipocytes were used to be transdifferentiated to osteoblast cell lines; and (3) the use and the construction of bio-scaffold sea marine coral and the fibrin gel were previewed. Eventually, both osteocell groups were collected and seeded onto a sea coral scaffold to confirm their capability of homing in a bone-like environment to consider the potential clinical application for hard tissue reconstruction. Also adipocytes were seeded onto a fibrin gel to generate a human fatlike tissue to be used in soft and semihard tissues such as subcutaneous adipose tissue, cartilages, and intervertebral disk bulbs. Results have been confirmed using specific molecular and biochemical analysis.

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Authors and Affiliations

  1. Biomaterial Research Laboratory, Department of Histology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach University of Medicine, 86/2 Thanh Thai St., 10th Dist., Ho Chi Minh City, Viet Nam

    Toai Cong Tran M.D., Ph.D

  2. Department of Histology – Anapathology, School of Medicine, Vietnam National University – Ho Chi Minh City, Quarter 6, Linh Trung Ward, Ho Chi Minh City, Thu Duc District, Viet Nam

    Toai Cong Tran M.D., Ph.D

  3. Department of Histology and Embryology, Faculty of Medicine, The University of Medicine and Pharmacy at Ho Chi Minh City, 217 Hong Bang St. 5th Dist., Ho Chi Minh City, Viet Nam

    Toai Cong Tran M.D., Ph.D

  4. Department of Histology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Viet Nam

    Ciro Gargiulo Ph.D., M.Sc. & Thao Duy Huynh M.A.

  5. University of Western Australia School of Anatomy and Human Biology, Perth, Australia

    Ciro Gargiulo Ph.D., M.Sc.

  6. Orthopaedic Department, University Medical Center, University of Medicine and Pharmacology, HCM City, 215–217 Hong Bang St., Dist.5, HCM City, Viet Nam

    Khanh Hong Thien Bui M.D., Ph.D

  7. School of Anatomy, Physiology and Human Biology, The University of Western Australia, 35 Stirling Highway, Perth, WA, M309, Australia

    Luis Filgueira M.D.

  8. Anatomy Unit, Department of Medicine, University of Fribourg, CH-1700, Fribourg, Switzerland

    Luis Filgueira M.D.

  9. Department of Orthopeadics and Sports Medicine, University of Washington School of Medicine, 18624 94th Ave West, Edmonds, WA, 98020, USA

    Douglas M. Strong Ph.D

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  1. Toai Cong Tran M.D., Ph.D
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  2. Ciro Gargiulo Ph.D., M.Sc.
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  3. Thao Duy Huynh M.A.
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  4. Khanh Hong Thien Bui M.D., Ph.D
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  5. Luis Filgueira M.D.
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  6. Douglas M. Strong Ph.D
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Correspondence to Toai Cong Tran M.D., Ph.D .

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Editors and Affiliations

  1. Section of Surgery, Newport Specialty Hospital, Tustin, California, USA

    Melvin A. Shiffman

  2. Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University of Ancona, Ancona, Italy

    Alberto Di Giuseppe

  3. Department of Plastic Surgery, University of Padova, Padova, Italy

    Franco Bassetto

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Tran, T.C., Gargiulo, C., Huynh, T.D., Bui, K.H.T., Filgueira, L., Strong, D.M. (2014). Adipocytes and Osteoblasts from Human Adipose Tissue Mesenchymal Stem Cells for the Production of Compatible and Safe Biomaterial Crucial in Cosmetic, Reconstructive, and Regenerative Medicine. In: Shiffman, M., Di Giuseppe, A., Bassetto, F. (eds) Stem Cells in Aesthetic Procedures. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45207-9_6

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  • DOI: https://doi.org/10.1007/978-3-642-45207-9_6

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