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Methods in Fungal Genetics

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Genomics of Soil- and Plant-Associated Fungi

Part of the book series: Soil Biology ((SOILBIOL,volume 36))

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Abstract

The myriad uses of filamentous and yeastlike fungi have spawned industry and investigation touching on every aspect of human endeavor. Much like the domestication of livestock and crop plants, humans have domesticated and improved fungi for specific and general use. In the modern biotechnological era, these improvements include strain characterization and standardization as well as strain improvement through genetic and molecular methods. Underpinning this improvement are biological materials whose shared use comprises a molecular toolkit for manipulation of filamentous fungi. These include various dominant and recessive selectable markers, gene targeting systems, protein expression and tagging vectors, and the mutated or engineered strains designed for these vectors. Some of these are based on features of the fungal lifestyle, while others were imported by analogy from bacterial, plant, or animal systems. Together they comprise a flexible and powerful molecular genetic framework for the development of highly productive strains and culture systems as fungi are used as foundations of the bio-economy of the twenty-first century.

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  1. Fungal Genetics Stock Center, University of Missouri, Kansas City, MO, USA

    Kevin McCluskey & Aric Wiest

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  1. Dep. of Biology, Technion – Israel Inst. of Technology, Haifa, Israel

    Benjamin A. Horwitz

  2. Central Institute for Cotton Research, Nagpur, India

    Prasun K. Mukherjee

  3. Central Institute for Cotton Research, Nagpur, India

    Mala Mukherjee

  4. Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria

    Christian P. Kubicek

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McCluskey, K., Wiest, A. (2013). Methods in Fungal Genetics. In: Horwitz, B., Mukherjee, P., Mukherjee, M., Kubicek, C. (eds) Genomics of Soil- and Plant-Associated Fungi. Soil Biology, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-39339-6_15

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