Abstract
Rhizopus oryzae is a zygomycetous fungus with both commercial and medical importance. It has been exploited for centuries for its fermentation capabilities and has also been harnessed commercially for its ability to produce a number of important organic compounds. Unfortunately, R. oryzae is also an important and growing human fungal pathogen that is capable of causing fatal infections. The recently completed genome sequence has provided some explanations for the dual importance of this fungus as both a pathogen and commercial asset. Basic research on R. oryzae at the molecular level has made steady progress and some important contributions have been made because of this research into the areas of both strain improvement and disease management. Continued progress is needed and new investigators entering the field are required in order to continue advancing what we know about this fungus.
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References
Abe A, Sone T, Sujaya IN, Saito K, Oda Y et al (2003) rDNA ITS sequence of Rhizopus oryzae: its application to classification and identification of lactic acid producers. Biosci Biotechnol Biochem 67:1725–1731
Abe A, Oda Y, Asano K, Sone T (2006) The molecular phylogeny of the genus Rhizopus based on rDNA sequences. Biosci Biotechnol Biochem 70:2387–2393
Abe A, Oda Y, Asano K, Sone T (2007) Rhizopus delemar is the proper name for Rhizopus oryzae fumaric-malic acid producers. Mycologia 99:714–722
Abe A, Asano K, Sone T (2010) A molecular phylogeny-based taxonomy of the genus Rhizopus. Biosci Biotechnol Biochem 74:1325–1331
Arnaiz-Garcia ME, Alonso-Pena D, Gonzalez-Vela Mdel C, Garcia-Palomo JD, Sanz-Gimenez-Rico JR et al (2009) Cutaneous mucormycosis: report of five cases and review of the literature. J Plast Reconstr Aesthet Surg 62:e434–e441
Artis WM, Fountain JA, Delcher HK, Jones HE (1982) A mechanism of susceptibility to mucormycosis in diabetic ketoacidosis: transferrin and iron availability. Diabetes 31:1109–1114
Bai DM, Zhao XM, Li XG, Xu SM (2004) Strain improvement of Rhizopus oryzae for over-production of (+)-lactic acid and metabolic flux analysis of mutants. Biochem Eng J 18:41–48
Balajee SA, Borman AM, Brandt ME, Cano J, Cuenca-Estrella M et al (2009) Sequence-based identification of Aspergillus, Fusarium, and mucorales species in the clinical mycology laboratory: where are we and where should we go from here? J Clin Microbiol 47:877–884
Boeke JD, LaCroute F, Fink GR (1984) A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197:345–346
Boelaert JR, de Locht M, Van Cutsem J, Kerrels V, Cantinieaux B et al (1993) Mucormycosis during deferoxamine therapy is a siderophore-mediated infection. In vitro and in vivo animal studies. J Clin Invest 91:1979–1986
Chen C, Pande K, French SD, Tuch BB, Noble SM (2011) An iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis. Cell Host Microbe 10:118–135
Datta R, Henry M (2006) Lactic acid: recent advances in products, processes and technologies—a review. J Chem Technol Biotechnol 81:1119–1129
Debets AJ, Swart K, Holub EF, Goosen T, Bos CJ (1990) Genetic analysis of amdS transformants of Aspergillus niger and their use in chromosome mapping. Mol Gen Genet 222:284–290
Ellis DH (1998) The zygomycetes. In: Ajello L, Hay RJ (eds) Medical mycology, 9th edn. Arnold, London, 711 pp
Erickson T, Liu L, Gueyikian A, Zhu X, Gibbons J et al (2001) Multiple virulence factors of Cryptococcus neoformans are dependent on VPH1. Mol Microbiol 42:1121–1131
Eucker J, Sezer O, Graf B, Possinger K (2001) Mucormycoses. Mycoses 44:253–260
Forgac M (1998) Structure, function and regulation of the vacuolar (H+)-ATPases. FEBS Lett 440:258–263
Fu J, Hettler E, Wickes BL (2006) Split marker transformation increases homologous integration frequency in Cryptococcus neoformans. Fungal Genet Biol 43:200–212
Ghosh B, Ray RR (2011) Current commercial perspective of Rhizopus oryzae: a review. J Appl Sci 11:2470–2486
Gryganskyi AP, Lee SC, Litvintseva AP, Smith ME, Bonito G et al (2010) Structure, function, and phylogeny of the mating locus in the Rhizopus oryzae complex. PLoS One 5:e15273
Haas H (2012) Iron – a key nexus in the virulence of Aspergillus fumigatus. Front Microbiol 3:28
Hachmeister KA, Fung DY (1993) Tempeh: a mold-modified indigenous fermented food made from soybeans and/or cereal grains. Crit Rev Microbiol 19:137–188
Hagensee ME, Bauwens JE, Kjos B, Bowden RA (1994) Brain abscess following marrow transplantation: experience at the Fred Hutchinson Cancer Research Center, 1984–1992. Clin Infect Dis 19:402–408
Hilty J, Smulian AG, Newman SL (2008) The Histoplasma capsulatum vacuolar ATPase is required for iron homeostasis, intracellular replication in macrophages and virulence in a murine model of histoplasmosis. Mol Microbiol 70:127–139
Hilty J, George Smulian A, Newman SL (2011) Histoplasma capsulatum utilizes siderophores for intracellular iron acquisition in macrophages. Med Mycol 49:633–642
Hinnen A, Hicks JB, Fink GR (1978) Transformation of yeast. Proc Natl Acad Sci U S A 75:1929–1933
Howard DH (1999) Acquisition, transport, and storage of iron by pathogenic fungi. Clin Microbiol Rev 12:394–404
Huang L, Wei P, Zang R, Xu Z, Cen P (2010) High-throughput screening of high-yield colonies of Rhizopus oryzae for enhanced production of fumaric acid. Ann Microbiol 60:287–292
Ibrahim AS (2011) Host cell invasion in mucormycosis: role of iron. Curr Opin Microbiol 14:406–411
Ibrahim AS, Spellberg B, Avanessian V, Fu Y, Edwards JE Jr (2005) Rhizopus oryzae adheres to, is phagocytosed by, and damages endothelial cells in vitro. Infect Immun 73:778–783
Ibrahim AS, Edwards JE Jr, Fu Y, Spellberg B (2006) Deferiprone iron chelation as a novel therapy for experimental mucormycosis. J Antimicrob Chemother 58:1070–1073
Ibrahim AS, Gebermariam T, Fu Y, Lin L, Husseiny MI et al (2007) The iron chelator deferasirox protects mice from mucormycosis through iron starvation. J Clin Invest 117:2649–2657
Ibrahim AS, Gebremariam T, Lin L, Luo G, Husseiny MI et al (2010) The high affinity iron permease is a key virulence factor required for Rhizopus oryzae pathogenesis. Mol Microbiol 77:587–604
Ibrahim AS, Spellberg B, Walsh TJ, Kontoyiannis DP (2012) Pathogenesis of mucormycosis. Clin Infect Dis 54(Suppl 1):S16–S22
Idnurm A, Walton FJ, Floyd A, Heitman J (2008) Identification of the sex genes in an early diverged fungus. Nature 451:193–196
Jung WH, Kronstad JW (2008) Iron and fungal pathogenesis: a case study with Cryptococcus neoformans. Cell Microbiol 10:277–284
Kelly JM, Hynes MJ (1985) Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans. EMBO J 4:475–479
Kwon-Chung KJ, Bennett JE (1992) Medical mycology. Lea and Febiger, Philadelphia, PA, 866 pp
Lanternier F, Dannaoui E, Morizot G, Elie C, Garcia-Hermoso D et al (2012) A global analysis of mucormycosis in France: the RetroZygo Study (2005–2007). Clin Infect Dis 54(Suppl 1):S35–S43
Lee SC, Corradi N, Byrnes EJ III, Torres-Martinez S, Dietrich FS et al (2008) Microsporidia evolved from ancestral sexual fungi. Curr Biol 18:1675–1679
Ma LJ, Ibrahim AS, Skory C, Grabherr MG, Burger G et al (2009) Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a whole-genome duplication. PLoS Genet 5:e1000549
Magee BB, Hube B, Wright RJ, Sullivan PJ, Magee PT (1993) The genes encoding the secreted aspartyl proteinases of Candida albicans constitute a family with at least three members. Infect Immun 61:3240–3243
McClelland CM, Wickes BL (2009) Agrobacterium tumefaciens as a molecular tool for the study of fungal pathogens. In: Rai M, Bridge PD (eds) Applied mycology. CAB International, Oxon, pp 239–257
Mertens JA, Skory CD, Ibrahim AS (2006) Plasmids for expression of heterologous proteins in Rhizopus oryzae. Arch Microbiol 186:41–50
Meussen BJ, de Graaff LH, Sanders JP, Weusthuis RA (2012) Metabolic engineering of Rhizopus oryzae for the production of platform chemicals. Appl Microbiol Biotechnol 94:875–886
Michielse CB, Salim K, Ragas P, Ram AF, Kudla B et al (2004) Development of a system for integrative and stable transformation of the zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer. Mol Genet Genomics 271:499–510
Monod M, Capoccia S, Lechenne B, Zaugg C, Holdom M et al (2002) Secreted proteases from pathogenic fungi. Int J Med Microbiol 292:405–419
Nairz M, Schroll A, Sonnweber T, Weiss G (2010) The struggle for iron – a metal at the host-pathogen interface. Cell Microbiol 12:1691–1702
Neame P, Rayner D (1960) Mucormycosis. A report on twenty-two cases. Arch Pathol 70:261–268
Nout MJ, Kiers JL (2005) Tempe fermentation, innovation and functionality: update into the third millenium. J Appl Microbiol 98:789–805
Oda Y, Saito K, Yamauchi H, Mori M (2002) Lactic acid fermentation of potato pulp by the fungus Rhizopus oryzae. Curr Microbiol 45:1–4
Oda Y, Yajima Y, Kinoshita M, Ohnishi M (2003) Differences of Rhizopus oryzae strain in organic synthesis and fatty acid composition. Food Microbiol 20:371–375
O’Gorman CM, Fuller HT, Dyer PS (2009) Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus. Nature 457:471–474
Poltermann S, Nguyen M, Gunther J, Wendland J, Hartl A et al (2005) The putative vacuolar ATPase subunit Vma7p of Candida albicans is involved in vacuole acidification, hyphal development and virulence. Microbiology 151:1645–1655
Purohit JS, Dutta JR, Nanda RK, Banerjee R (2006) Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae. Bioresour Technol 97:795–801
Rabie NB, Althaqafi AO (2012) Rhizopus-associated soft tissue infection in an immunocompetent air-conditioning technician after a road traffic accident: a case report and review of the literature. J Infect Public Health 5:109–111
Rammaert B, Lanternier F, Zahar JR, Dannaoui E, Bougnoux ME et al (2012) Healthcare-associated mucormycosis. Clin Infect Dis 54(Suppl 1):S44–S54
Record NB Jr, Ginder DR (1976) Pulmonary phycomycosis without obvious predisposing factors. JAMA 235:1256–1257
Ribes JA, Vanover-Sams CL, Baker DJ (2000) Zygomycetes in human disease. Clin Microbiol Rev 13:236–301
Rinaldi MG (1989) Zygomycosis. Infect Dis Clin North Am 3:19–41
Roden MM, Zaoutis TE, Buchanan WL, Knudsen TA, Sarkisova TA et al (2005) Epidemiology and outcome of zygomycosis: a review of 929 reported cases. Clin Infect Dis 41:634–653
Romano AH, Bright MM, Scott WE (1967) Mechanism of fumaric acid accumulation in Rhizopus nigricans. J Bacteriol 93:600–604
Saito K, Saito A, Ohnishi M, Oda Y (2004) Genetic diversity in Rhizopus oryzae strains as revealed by the sequence of lactate dehydrogenase genes. Arch Microbiol 182:30–36
Schipper MAA (1984) A revision of the genus Rhizopus I: the Rhizopus stolonifer-group and Rhizopus oryzae. Stud Mycol 25:1–19
Schipper MAA, Stalpers JA (1984) A revision of the genus Rhizopus. Stud Mycol 25:1–34
Scholer HJ, Muller E, Schipper MAA (1983) Mucorales. In: Howard DH (ed) Fungi pathogenic for humans and animals. Marcel Dekker, New York
Skory CD (2000) Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Appl Environ Microbiol 66:2343–2348
Skory CD (2002) Homologous recombination and double-strand break repair in the transformation of Rhizopus oryzae. Mol Genet Genomics 268:397–406
Skory CD (2003) Induction of Rhizopus oryzae pyruvate decarboxylase genes. Curr Microbiol 47:59–64
Skory CD (2004) Lactic acid production by Rhizopus oryzae transformants with modified lactate dehydrogenase activity. Appl Microbiol Biotechnol 64:237–242
Skory CD, Ibrahim AS (2007) Native and modified lactate dehydrogenase expression in a fumaric acid producing isolate Rhizopus oryzae 99-880. Curr Genet 52:23–33
Skory CD, Freer SN, Bothast RJ (1998) Production of L-lactic acid by Rhizopus oryzae under oxygen limiting conditions. Biotechnol Lett 20:191–194
Steinkraus KH (1985) Indigenous fermented-food technologies for small-scale industries. Food Nutr Bull 7:21–27
Sugui JA, Losada L, Wang W, Varga J, Ngamskulrungroj P et al (2011) Identification and characterization of an Aspergillus fumigatus “supermater” pair. MBio 2:e00234-11
Suntornsuk W, Hang YD (2008) Strain improvement of Rhizopus oryzae for production of l(+)-lactic acid and glucoamylase. Lett Appl Microbiol 19:249–252
Van den Saffele JK, Boelaert JR (1996) Zygomycosis in HIV-positive patients: a review of the literature. Mycoses 39:77–84
Vitale RG, de Hoog GS, Schwarz P, Dannaoui E, Deng S et al (2012) Antifungal susceptibility and phylogeny of opportunistic members of the order mucorales. J Clin Microbiol 50:66–75
Zaoutis TE, Roilides E, Chiou CC, Buchanan WL, Knudsen TA et al (2007) Zygomycosis in children: a systematic review and analysis of reported cases. Pediatr Infect Dis J 26:723–727
Zheng Z, Luo SZ, Li XJ, Wu XF, Pan LJ et al (2009) Screening of allyl alcohol resistant mutant of Rhizopus oryzae and its fermentation characterization. Afr J Biotechnol 8:280–284
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Wickes, B.L. (2013). Rhizopus oryzae: Genetic Secrets of an Emerging Human Pathogen. In: Horwitz, B., Mukherjee, P., Mukherjee, M., Kubicek, C. (eds) Genomics of Soil- and Plant-Associated Fungi. Soil Biology, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-39339-6_11
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