Skip to main content
SpringerLink
Log in
Book cover

Yarrowia lipolytica pp 121–136Cite as

Biotechnological Applications of Yarrowia lipolytica Lipases: An Overview

  • Chapter
  • First Online:

Part of the book series: Microbiology Monographs ((MICROMONO,volume 25))

Abstract

The yeast Yarrowia lipolytica has the ability to grow on substrates such as lipid or grease. The first step in the breakdown and metabolism of those hydrophobic substrates requires the actions of lipolytic enzymes. Lipases from Y. lipolytica, and particularly the lip2 enzyme, possess amazing properties in both aqueous and nonaqueous media. Those catalytic properties have enabled the development of many applications. Oil mill wastewater represents a serious risk of pollution due to its high contents of organic compounds, mainly fatty acids and triglycerides. Besides the physicochemical processes developed to treat those wastewaters, the Lip2p lipase was found as an attractive and low-cost biological alternative. In that field, they were also used successfully to degrade sludge for grease trap from the food industries. Applications were also developed in fine chemistry due to the abilities of Lip2p as an enantioselective catalyzer in organic medium for the synthesis of pharmaceutical drug used as a single enantiomer. Besides this, Lip2p was also found effective in the polymerization and modification processes of different fatty compounds of industrial interest. Microorganisms and their produced enzymes are also known for their role in traditional food making. The role of the lipase enzyme from Y. lipolytica in flavor development in cheese and fermented sausage is detailed in this review. Their involvement in the production of organic acids such as citric acid from hydrophobic compounds is also presented.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Adams S, Bresloff P, Mason C (1976) Pharmacological difference between the optical isomers of ibuprofen: evidence for metabolic inversion of the (-)isomer. J Pharm Pharmacol 28:256–257

    Article  PubMed  CAS  Google Scholar 

  • Addis E, Fleet G, Cox JM (1998) Evaluation of methods for the isolation and enumeration of yeast in mould-ripened cheese. In: Jakobsen M, Narvhus J, Viljoen BC (eds) Yeast in the dairy industry: positive and negative aspects. International Dairy Federation, Brussels, pp 109–116

    Google Scholar 

  • Al-Malah K, Azzam M, Abu-lail N (2000) Olive mills effluent (OME) wastewater post treatment using activated clay. Sep Purif Technol 20:225–234

    Article  CAS  Google Scholar 

  • Andreoni V, Bonfanti P, Daffonchio D, Sorlini C, Villa M (1993) Anaerobic-digestion of olive mill-effluent: microbiological and processing aspects. J Environ Health Part A 28:2041–2059

    Google Scholar 

  • Ansenne A, Destain J, Godefroid J, Thonart P (1992) La problématique des séparateur de graisse. Trib eau 558:33–40

    Google Scholar 

  • Barrera-Rivera K, Flores-Carreon A, Martinez-Richa A (2008) Enzymatic ring opening polymerization of ε-caprolactone by a new lipase from Yarrowia lipolytica. J Appl Pol Sci 109:708–719

    Article  CAS  Google Scholar 

  • Berdague JL, Monteil P, Montel M, Talon R (1993) Effects of starter cultures on the formation of flavour compounds in dry sausage. Meat Sci 35:275–287

    Article  PubMed  CAS  Google Scholar 

  • Bordes F, Cambon E, Dossat-Letisse V, Andre I, Croux C, Nicaud JM, Marty A (2009) Improvement of Yarrowia lipolytica lipase enantioselectivity by using mutagenesis targeted to the substrate binding site. Chembiochem 10:1705–1713

    Article  PubMed  CAS  Google Scholar 

  • Breuer M, Ditrich K, Habicher T, Hauer B, Kesseler M, Stuurmer R, Zelinski T (2004) Industrial methods for the production of optically active intermediates. Angew Chem Int Ed 43:788–824

    Article  CAS  Google Scholar 

  • Briand D, Dubreucq E, Galzy P (1994) Enzymatic fatty esters synthesis in aqueous medium with lipase from Candida parapsilosis (Ashford) langeron and talice. Biotechnol Lett 16:813–818

    Article  CAS  Google Scholar 

  • Chin K, Lee S, Mohammad H (1996) A study of palm oil mill effluent treatment using a pond system. Water Sci Technol 34:119–123

    CAS  Google Scholar 

  • Cossu R, Blakey N, Cannas P (1993) Influence of codisposal of municipal solid-waste and oil vegetation water on the anaerobic digestion of a sanitary-landfill. Water Sci Technol 27:261–271

    Article  CAS  Google Scholar 

  • D’Annibale A, Crestini C, Vinciguerra V, Sermanni GG (1998) The biodegradation of recalcitrant effluents from an olive mill by a white-rot fungus. J Biotechnol 61:209–218

    Article  PubMed  Google Scholar 

  • D’Annibale A, Ricci M, Quaratino D, Federici F, Fenice M (2004) Panus tigrinus efficiently removes phenols, color and organic load from olive-mill wastewater. Res Microbiol 155:596–603

    Article  PubMed  Google Scholar 

  • Destain J, Roblain D, Thonart P (1997) Improvement of lipase production from Yarrowia lipolytica. Biotechnol Lett 19:105–107

    Article  CAS  Google Scholar 

  • De Felice B, Pontecorvo G, Carfagna M (1997) Degradation of waste waters from olive oil mills by Yarrowia lipolytica ATCC 20255 and Pseudomonas putida. Acta Biotechnol 17:231–239

    Article  Google Scholar 

  • Dominguez A, Longo M, Sanroman M (2005) Recycling of waste cooking oil for lipase production by Yarrowia lipolytica. J Biotechnol 118S1:S170

    Google Scholar 

  • Esmelindro A, Fiametti K, Ceni G, Corazza M, Treichel H, de Oliveira D, Oliveira J (2008) Lipase-catalysed production of monoglycerides in compressed propane and AOT surfactant. J Suercrit Fluids 47:64–69

    Article  CAS  Google Scholar 

  • Fickers P, Benetti PH, Wache Y, Marty A, Mauersberger S, Smit MS, Nicaud JM (2005) Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res 5:527–543

    Article  PubMed  CAS  Google Scholar 

  • Gardini F, Suzzi G, Lombardi A, Galgano F, Crudele MA, Andrighetto C, Schirone M, Tofalo R (2001) A survey of yeasts in traditional sausages of southern Italy. FEMS Yeast Res 1:161–167

    Article  PubMed  CAS  Google Scholar 

  • Goncalves C, Lopes M, Ferreira J, Belo I (2009) Biological treatment of olive mill wastewater by non-conventional yeast. Bioresour Technol 100:3759–3763

    Article  PubMed  CAS  Google Scholar 

  • Guieysse D, Salagnad C, Monsan P, Remaud-Simeon M (2003a) Lipase-catalyzed enantioselective transesterification toward esters of 2-bromo-tolylacetic acids. Tetrahedron Asymmetry 14:317–323

    Article  CAS  Google Scholar 

  • Guieysse D, Salagnad C, Monsan P, Remaud-Simeon M (2003b) Towards a novel explanation of Pseudomonas cepacia lipase enantioselectivity via molecular modelling of the enantiomer trajectory into the active site. Tetrahedron Asymmetry 14:1807–1817

    Article  CAS  Google Scholar 

  • Guieysse D, Sandoval G, Faure L, Nicaud J, Monsan P, Marty A (2004) New efficient lipase from Yarrowia lipolytica for the resolution of 2-bromo-arylacetic acid esters. Tetrahedron Asymmetry 15:3539–3543

    Article  CAS  Google Scholar 

  • Hirai M (2001) New advanced technology for fish waste. Food Dev 35:11–13

    Google Scholar 

  • Hutt A, Caldwell J (1984) The importance of stereochemistry in the clinical pharmacokinetics of the 2-arylpropionic acid non-steroidal anti-inflammatory drug. Clin Pharmacokinet 9:371–373

    Article  PubMed  CAS  Google Scholar 

  • Juaristi E, Leon-Romo J, Reyes A, Escalante J (1999) Recent applications of alpha-phenylalanine in the preparation of enantiopure compounds. Tetrahedron Asymmetry 10:2441–2495

    Article  CAS  Google Scholar 

  • Kamzolova S, Morgunov I, Aurich A, Perevoznikova O, Shishkanova N, Stottmeister U, Finogenova T (2005) Lipase secretion and citric acid production in Yarrowia lipolytica yeast grown on animal and vegetable fat. Food Technol Biotechnol 43:113–122

    CAS  Google Scholar 

  • Kamzolova S, Finogenova T, Lunina O, Perevoznikova O, Minachova L, Morgunov I (2007) Characteristics of the growth on rapeseed oil and synthesis of citric and isocitric acids by Yarrowia lipolytica yeasts. Microbiology 76:20–24

    Article  CAS  Google Scholar 

  • Klasson T, Clausen J, Gaddy J (1989) Continuous fermentation for the production of citric acid from glucose. Appl Biochem Biotechnol 23:491–509

    Article  Google Scholar 

  • Kristiansen B, Sinclair C (1979) Production of citric acid in continuous culture. Biotechnol Bioeng 21:297–315

    Article  CAS  Google Scholar 

  • Lanciotti R, Gianotti A, Baldi D, Angrisani R, Suzzi G, Mastrocola D, Guerzoni M (2005) Use of Yarrowia lipolytica strains for the treatment of olive mill wastewater. Bioresour Technol 96:317–322

    Article  PubMed  CAS  Google Scholar 

  • Liu Y, Wanf F, Tan T (2009) Cyclic resolution of racemic ibuprofen via coupled efficient lipase and acid-base catalysis. Chirality 21:349–353

    Article  PubMed  CAS  Google Scholar 

  • Lopes M, Araujo C, Aguedo M, Gomes N, Goncalves C, Teixeira J, Belo I (2009) The use of olive mill wastewater by wild-type Yarrowia lipolytica strains: medium supplementation and surfactant presence effect by increased air pressure. J Chem Technol Biotechnol 84:533–537

    Article  CAS  Google Scholar 

  • Mameri N, Halet F, Drouiche M, Grib H, Lounici H, Pauss A, Piron D, Belhocine D (2000) Treatment of olive mill washing water by ultrafiltration. Can J Chem Eng 78:590–595

    Article  CAS  Google Scholar 

  • O’Donnell M (2001) The preparation of optically active alpha-amino acids from the benzophenone imines of glycine derivatives. Aldrichim Acta 34:3–15

    Google Scholar 

  • Oswal N, Sarma PM, Zinjarde S, Pant A (2002) Palm oil mill effluent treatment by tropical marine yeast. Bioresour Technol 85:35–37

    Article  PubMed  CAS  Google Scholar 

  • Papanikolaou S, Galiotou-Panayotou M, Fakas S, Komaitis M, Aggelis G (2008) Citric acid production by Yarrowia lipolytica cultivated on olive-mill wastewater-based media. Bioresour Technol 99:2419–2428

    Article  PubMed  CAS  Google Scholar 

  • Peltsmane I, Raminya L, Finogenova T, Karklin R (1988) Biosynthesis and isolation of isocitric acide from n-alkane solution. Izv An Latv 487:93–95

    Google Scholar 

  • Rane K, Sims K (1993) Production of citric acid by Candida lipolytica Y1905: effect of glucose concentration on the yield and productivity. Enzyme Microb Technol 15:646–651

    Article  CAS  Google Scholar 

  • Ros de Ursinos F (1961) Estudio del alpechin para su aprovechamiento industrial. VI. Aminoacidos presentes en la levardura Torulopsis utilis. Gras Aceit 12:161–165

    Google Scholar 

  • Saito H (2004) Lipid and FA composition of the pearl oyster Pinctada fucata martensii: influence of season and maturation. Lipids 39:997–1005

    Article  PubMed  CAS  Google Scholar 

  • Samelis J, Aggelis G, Metaxopoulos J (1993) Lipolytic and microbial changes during the natural fermentation and ripening of Greek dry sausages. Meat Sci 35:371–385

    Article  PubMed  CAS  Google Scholar 

  • Scioli C, De Felice B (1993) Impiega de ceppi di lievito nella depurazione dei feflui dell’industria olearia. Ann Microbiol Enzymol 43:61–69

    CAS  Google Scholar 

  • Scioli C, Vollaro L (1997) The use of Yarrowia lipolytica to reduce pollution in olive mill wastewaters. Water Res 31:2520–2524

    Article  CAS  Google Scholar 

  • Suzzi G, Larnote M, Galgano F, Andrighetto C, Lombardi A, Lanciotti R, Guerzoni M (2001) Proteolytic, lipolytic and molecular characterisation of Yarrowia lipolytica isolated from cheese. Int J Food Microbiol 69:69–77

    Article  PubMed  CAS  Google Scholar 

  • Thonart P, Destain J, Zgoulli S, Antoine P, Godefroid J, Evrard P (1997) Les bacs à graisse: une solution aux problèmes des matières grasses pour les PME. Trib eau 586:41–46

    Google Scholar 

  • Tsai S, Lin J, Chang C, Chen J (1997) Enzymatic synthesis of (S)-ibuprofen ester prodrug from racemic ibuprofen by lipase in organic solvents. Biotechnol Prog 13:82–87

    Article  CAS  Google Scholar 

  • Wen S, Tan T, Yu M (2008) Immobilized lipase YlLip2-catalysed resolution of alpha-phenylethyl amine in a medium with organic cosolvent. Process Biochem 43:1259–1264

    Article  CAS  Google Scholar 

  • Wojtatowicz M, Rymowicz W, Kautola H (1991) Comparison of different strains of the yeast Yarrowia lipolytica for citric acid production from glucose hydrol. Appl Biochem Biotechnol 31:165–174

    Article  PubMed  CAS  Google Scholar 

  • Wu L, Wan J (2008) Investigation on the capability of disposing the grease wastewater with immobilized Yarrowia lipolytica. Chin J Environ Eng 4:482–486

    Google Scholar 

  • Wu L, Ge G, Wan J (2009) Biodegradation of oil wastewater by free and immobilized Yarrowia lipolytica W29. J Environ Sci 21:237–242

    Article  CAS  Google Scholar 

  • Wyder M, Bachmann HP, Puhan Z (1999) Role of selected yeasts in cheese ripening: an evaluation in foil wrapped raclette cheese. Lebensm Wiss Technol 32:333–343

    Article  CAS  Google Scholar 

  • Yano Y, Oikawa H, Satomi M (2008) Reduction of lipids in fish meal prepared from fish waste by a yeast Yarrowia lipolytica. Int J Food Microbiol 121:302–307

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unité de Biotechnologies et de Bioprocédés, Université Libre de Bruxelles, Av. F.-D. Roosevelt 50, C.P. 165/61, 1050, Bruxelles, Belgium

    Patrick Fickers

  2. CNRS, Institut MICALIS, Domaine de Vilvert, 78352, Jouy-en-Josas, France

    Jean-Marc Nicaud

Authors
  1. Patrick Fickers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean-Marc Nicaud
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patrick Fickers .

Editor information

Editors and Affiliations

  1. TU Dresden Inst. Mikrobiologie, Dresden, Sachsen, Germany

    Gerold Barth

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Fickers, P., Nicaud, JM. (2013). Biotechnological Applications of Yarrowia lipolytica Lipases: An Overview. In: Barth, G. (eds) Yarrowia lipolytica. Microbiology Monographs, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38583-4_4

Download citation

Publish with us

Policies and ethics

Search

Navigation