Abstract
Utilization of n-alkane and its oxidized derivatives as sources of carbon and energy is one of the important properties that characterize the yeast Yarrowia lipolytica and has been a subject of intensive study. We describe a progress that has been made in the last decade, mainly on the transcriptional control of ALK genes encoding 12 CYP52 family cytochromes P450ALK that work in initial hydroxylation of n-alkanes. Among 12 ALK genes, ALK1 is most prominently expressed upon the addition of n-alkanes, where two positive regulators, Yas1p and Yas2p, bind together to the promoter element ARE1 and highly activate transcription of ALK1. The control by n-alkane depends on the localization of a negative regulator Yas3p that binds to Yas2p in nucleus when n-alkanes are absent but remains binding to ER when n-alkanes are present in culture medium.
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Fukuda, R., Ohta, A. (2013). Utilization of Hydrophobic Substrate by Yarrowia lipolytica . In: Barth, G. (eds) Yarrowia lipolytica. Microbiology Monographs, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38320-5_5
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DOI: https://doi.org/10.1007/978-3-642-38320-5_5
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