Abstract
Glycerol is the main by-product in ethanol production during the very high gravity (VHG) fermentation process by Saccharomyces cerevisiae. This study investigates the effect of GPD1 or GPD2 (encoding 3-phosphate dehydrogenase) deletion on the production of glycerol and ethanol through the VHG fermentation. We observed that deletion of GPD1 resulted in 45.30 % reduction in glycerol production compared with the parent strain, and ethanol production reached the levels of 15.8 ± 0.03 (v/v), while we failed to observe such a significant decrease in glycerol production for the GPD2 deletion mutants whose ethanol production was 15.7 ± 0.03 (v/v). It can be concluded that deletion of either GPD1 or GPD2 can elevate ethanol production, and that GPD1 deletion can significantly reduce glycerol production, suggesting that GPD1 plays a dominant role in regulating glycerol synthesis during the process of VHG fermentation.
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Acknowledgments
This work was financially supported by the program of National High Technology Research and Development Program of China (863 Program) (Grant No. SS2012AA023408), the Cheung Kong Scholars and Innovative Research Team Program in University of Ministry of Education, China (Grant No. IRT1166), and Tianjin Municipal High School Science and Technology Development Fund Program, China (No. 20110625).
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Yu, J., Dong, J., Zhang, C., Li, J., Xiao, D. (2014). Effect of GPD1 and GPD2 Deletion on the Production of Glycerol and Ethanol in the Yeast Saccharomyces cerevisiae . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_18
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DOI: https://doi.org/10.1007/978-3-642-37916-1_18
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