Abstract
Isolation and analysis of single DNA fragment are of great importance for both fundamental research and future biomedical applications. In this chapter, we introduce a technique based on atomic force microscope (AFM) to manipulate and isolate individual DNA molecules at the nanometer scale. The AFM was used to site specifically cut, push, and pick up single DNA fragments from solid surfaces. Subsequent amplification of the isolated single DNA fragments indicates that the DNA molecules keep their bioactivity after AFM manipulation. We believe that new applications will continue to be developed to further expand our repertoire of the AFM-based nanomanipulation techniques.
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Acknowledgements
This work was supported by the National Science Foundation of China (No. 10,975,175 and 90,923,002) and the Chinese Academy of Sciences (No. KJCX2-EW-N03). Y. Zhang thanks the Max Planck Society for support of a partner group.
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Zhang, Y., Hu, J. (2013). Manipulation and Isolation of Individual DNA Molecules with Atomic Force Microscope. In: Fan, C. (eds) DNA Nanotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36077-0_5
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DOI: https://doi.org/10.1007/978-3-642-36077-0_5
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