Abstract
The application of various microscopy methods—from stereomicroscopy to luminescence microscopy, microspectrofluorimetry and laser-scanning confocal microscopy—has been considered as an approach to model the cell–cell contacts and interactions in allelopathy. It bases on the direct observations of both secretions released from allelopathic species and the interaction(s) with the cell acceptors as biosensors (unicellular plant generative and vegetative microspores). Special attention was paid to the interactions with pigmented and fluorescing components of the secretions released by the cell donors from allelopathically active plant species. Colored allelochemicals are considered as histochemical dyes for the analysis of cellular mechanisms at the allelopathic contacts.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aliotta G, Cafiero G (1999) Biological properties of rue (Ruta graveolens L.). Potential use in sustainable agricultural systems. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 551–563
Alstyne KL, Nelson AV, Vyvyan JR, Cancilla DA (2006) Dopamine functions as an antiherbivore defense in the temperate green alga Ulvaria obscura. Oecologia 148:304–311
Gaur S, Rana A, Chauhan SVS (2007) Pollen allelopathy: past achievements and future approach. Allelopathy J 20:115–126
Gerbach PV (2002) The essential oil secreting structures of Prostanthera ovalifolia (Lamiaceae). Ann Bot 89:255–260
Gilroy S (1997) Fluorescence microscopy of living plant cells. Annu Rev Plant Physiol Plant Mol Biol 48:165–190
Golovkin BN, Rudenskaya RN, Trofimova IA, Shreter AI (2001) Biologically active substances of plant origin, vol 3. Nauka, Moscow
Hazak O, Bloch D, Poraty L, Stemberg H, Zhang J, Friml J, Yalovsky S (2010) A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution. PLoS Biol 8(1):e1000282. doi:10.1371/journal.pbio.1000282
Huang X, Jiang H, Hao G (2009) Direct HPLC detection of benzodilactones and quinones in glands of Lysimachia fordiana. Fitoterapia 80:173–176
Jaldappagari S, Motohashi N, Gangeenahalli MP, Naismith JH (2008) Bioactive mechanism of interaction between anthocyanins and macromolecules like DNA and proteins. Topics Heterocycl Chem 15:49–65
Karnaukhov VN, Yashin VA, Kulakov VI, Vershinin VM, Dudarev VV (1982) Apparatus for investigation of fluorescence characteristics of microscopic objects. US Patent, N4, 354, 114:1–14
Karnaukhov VN, Yashin VA, Kulakov VI, Vershinin VM, Dudarev VV (1983) Apparatus for investigation of fluorescence characteristics of microscopic objects. Patent of England 2.039.03 R5R.CHI
Karnaukhov VN, Yashin VA, Kazantsev AP, Karnaukhova NA, Kulakov VI (1987) Double-wave microfluorimeter-photometer based on standard attachment. Tsitologia (Cytology, USSR) 29:113–116
Karnaukhova NA, Sergievich LA, Karnaukhov VN (2010) Application of microspectral analysis to study intracellular metabolism in single cells and cell systems. Nat Sci 2:444–449
Mathesius U, Bayliss C, Weinman JJ, Schlaman HRM, Spaink HP, Rolfe BG, McCully ME, Djordjevic MA (1998) Flavonoids synthesized in cortical cells during nodule initiation are early developmental markers in white clover. Molec Plant Microbe Interact 11(12):1223–1232
Murphy SD (1992) The determination of allelopathic potential of pollen and nectar. In: Linskens HF, Jackson IF (eds) Plant toxins analysis. Springer, Berlin, pp 333–357
Murphy SD (1999) Pollen allelopathy. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 129–148
Murphy SD (2007) Allelopathic pollen: isolating the allelopathic effects. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 185–198
Pacek A, Stpiczynska M (2007) The structures of elaiophores of Oncidium cheirophorum Rchb.F. and Ornithocephalus kruegeri Rchb.F. (Orchidaceae). Acta Agrobot 60:9–14
Pawley J, Pawley JB (2006) Handbook of biological confocal microscopy. Springer, Berlin
Roshchina VV (1999) Mechanisms of cell–cell communication. In: Narwal SS (ed) Allelopathy update, vol 2. Science Publishers, Enfield, pp 3–25
Roshchina VV (2001a) Neurotransmitters in plant life. Science Publisher, Enfield
Roshchina VV (2001b) Molecular-cellular mechanisms in pollen alllelopathy. Allelopathy J 8:11–28
Roshchina VV (2002) Rutacridone as a fluorescent dye for the study of pollen. J Fluoresc 12:241–243
Roshchina VV (2003) Autofluorescence of plant secreting cells as a biosensor and bioindicator reaction. J Fluoresc 13:403–420
Roshchina VV (2004) Cellular models to study the allelopathic mechanisms. Allelopathy J 13:3–16
Roshchina VV (2005) Allelochemicals as fluorescent markers, dyes and probes. Allelopathy J 16:31–46
Roshchina VV (2006a) Plant microspores as biosensors. Trends Modern Biol 126:262–274
Roshchina VV (2006b) Chemosignaling in plant microspore cells. Biol Bull 33:414–420
Roshchina VV (2007a) Cellular models as biosensors. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 5–22
Roshchina VV (2007b) Luminescent cell analysis in allelopathy. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 103–115
Roshchina VV (2008) Fluorescing world of plant secreting cells. Science Publisher, Enfield
Roshchina VV, Karnaukhov VN (2010) The fluorescence analysis of the medicinal drugs’ interaction with unicellular biosensors. Pharmacia (Russia) 3:43–46
Roshchina VV, Melnikova EV (1995) Spectral analysis of intact secretory cells and excretions of plants. Allelopathy J 2:179–188
Roshchina VV, Melnikova EV (1996) Microspectrofluorometry: a new technique to study pollen allelopathy. Allelopathy J 3:51–58
Roshchina VV, Melnikova EV (1999) Microspectrofluorimetry of intact secreting cells, with applications to the study of allelopathy. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 99–126
Roshchina VV, Melnikova EV (1998) Allelopathy and plant generative cells. Participation of acetylcholine and histamine in a signalling at the interactions of pollen and pistil. Allelopathy J 5:171–182
Roshchina VV, Roshchina VD (1993) The excretory function of higher plants. Springer, Berlin
Roshchina VV, Melnikova EV, Spiridonov NA, Kovaleva LV (1995) Azulenes, the blue pigments of pollen. Doklady Biol Sci 340:93–96
Roshchina VV, Melnikova EV, Kovaleva LV (1996) Autofluorescence in system pollen-pistil of Hippeastrum hybridum. Doklady Biol Sci 349:118–120
Roshchina VV, Melnikova EV, Karnaukhov VN, Golovkin BN (1997) Application of microspectrofluorimetry in spectral analysis of plant secretory cells. Biol Bull (Russia) 2:167–171
Roshchina VV, Melnikova EV, Mit’kovskaya LI, Karnaukhov VN (1998) Microspectrofluorimetry for the study of intact plant secretory cells. J Gen Biol (Russia) 59:531–554
Roshchina VV, Melnikova EV, Yashin VA, Karnaukhov VN (2002) Autofluorescence of intact spores of horsetail Equisetum arvense L. during their development. Biophysics (Russia) 47:318–324
Roshchina VV, Yashin VA, Kononov AV (2004) Autofluorescence of plant microspores studied by confocal microscopy and microspectrofluorimetry. J Fluoresc 14:745–750
Roshchina VV, Yashin VA, Kononov AV, Yashina AV (2007) Laser-scanning confocal microscopy (LSCM): study of plant secretory cells. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 93–102
Roshchina VV, Yashina AV, Yashin VA (2008) Cell communication in pollen allelopathy analyzed with laser-scanning confocal microscopy. Allelopathy J 21:219–226
Roshchina VV, Yashina AV, Yashin VA, Prizova NK (2009a) Models to study pollen allelopathy. Allelopathy J 23:3–24
Roshchina VV, Yashin VA, Yashina AV, Gol’tyaev MV, Manokhina IA (2009b) Microscopic objects for the study of chemosignaling. In: Zinchenko VP, Kolesnikov SS, Berezhnov AV (eds) Reception and intracellular signalling. Biological Center of RAS, Pushchino, pp 699–703
Roshchina VV, Yashina AV, Yashin VA, Gol’tyaev MV (2011a) Fluorescence of biologically active compounds in plant secretory cells. In: Narwal SS, Pavlovic P, Jacob J (eds) Research methods in plant science, vol 2., Forestry and AgroforestryStudium Press, Houston, pp 3–25
Roshchina VV, Yashin VA, Yashina AV, Gol’tyaev MV (2011b) Colored allelochemicals in modelling of cell–cell allelopathic interactions. Allelopathy J 28:1–12
Roshchina VV, Yashin VA, Vikhlyantsev IM (2011c) Fluorescence of plant microspores as biosensors. Biol Membr 28:1–12
Roy S, Bhattacharya S, Das P, Chattopadhyay J (2007) Interaction among non-toxic phytoplankton, toxic phytoplankton and zooplankton: inferences from field observations. J Biol Phys 33:1–17
Salih A, Jones A, Bass D, Cox G (1997) Confocal imaging of exine for grass pollen analysis. Grana 36:215–224
Sharma AD, Sharma R (1999) Anthocyanin-DNA copigmentation complex: mutual protection against oxidative damage. Phytochem 52:1313–1318
Solé J, García-Ladona E, Ruardij P, Estrada M (2005) Modelling allelopathy among marine alga. Ecol Model 183:373–384
Stanley RG, Linskens HF (1974) Pollen, biology, biochemistry, managements. Springer, Berlin
Wymer CL, Beven AF, Boudonck K, Lloyd CW (1999) Confocal microscopy of plant cells. Methods Molec Biol 122:103–130
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Roshchina, V.V., Yashin, V.A., Yashina, A.V., Goltyaev, M.V. (2013). Microscopy for Modeling of Cell–Cell Allelopathic Interactions. In: Cheema, Z., Farooq, M., Wahid, A. (eds) Allelopathy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30595-5_17
Download citation
DOI: https://doi.org/10.1007/978-3-642-30595-5_17
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-30594-8
Online ISBN: 978-3-642-30595-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)