Methods for the Detection of Gasotransmitters

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Abstract

The discovery of gasotransmitters, such as nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S), represents great milestones in biology. Both the discovery and related study of these gasotransmitters have benefited from selective and sensitive detection methods. This chapter has briefly reviewed and compared the methods used for the detection and determination of these three gasotransmitters. For NO, the detection methods include small molecule organic chemoprobes, transition metal-based probes, capillary electrophoresis (CE), NO-selective electrodes, and protein-based probes. For H2S, the detection methods include chromatographic methods such as gas chromatography (GC) and high performance liquid chromatography (HPLC), reaction-based spectrophotometric methods such as fluorescent chemoprobes and electrochemical methods. CO detection in biological systems mainly focuses on measurement of carboxyhemoglobin (COHb) saturation. Methods such as spectrophotometric methods, GC, and electrochemical methods are used in this field.