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Culture Negative Orthopedic Biofilm Infections pp 61–75Cite as

Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention

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Part of the book series: Springer Series on Biofilms ((BIOFILMS,volume 7))

Abstract

Increasing attention has been focused on understanding, diagnosing, and treating nonculturable bacterial infections. In this chapter, we explore the current immunological methods for diagnosis and prevention of recalcitrant biofilm-associated nonculturable infections in the context of the Gram-positive cocci, Staphylococcus aureus. In addition, we discuss immune evasion strategies of S. aureus in the perspective of bacteria and host, and the laboratory techniques utilized for translational research and vaccine development.

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References

  • Akira S (1999) Functional roles of STAT family proteins: lessons from knockout mice. Stem Cells 17:138–146

    Article  PubMed  CAS  Google Scholar 

  • Anwar S, Prince LR, Foster SJ, Whyte MK, Sabroe I (2009) The rise and rise of Staphylococcus aureus: laughing in the face of granulocytes. Clin Exp Immunol 157:216–224

    Article  PubMed  CAS  Google Scholar 

  • Boles BR, Horswill AR (2008) Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 4:e1000052

    Article  PubMed  Google Scholar 

  • Boyd A, Chakrabarty AM (1994) Role of alginate lyase in cell detachment of Pseudomonas aeruginosa. Appl Environ Microbiol 60:2355–2359

    PubMed  CAS  Google Scholar 

  • Brady RA, Leid JG, Camper AK, Costerton JW, Shirtliff ME (2006) Identification of Staphylococcus aureus proteins recognized by the antibody-mediated immune response to a biofilm infection. Infect Immun 74:3415–3426

    Article  PubMed  CAS  Google Scholar 

  • Brady RA, Leid JG, Kofonow J, Costerton JW, Shirtliff ME (2007) Immunoglobulins to surface-associated biofilm immunogens provide a novel means of visualization of methicillin-resistant Staphylococcus aureus biofilms. Appl Environ Microbiol 73:6612–6619

    Article  PubMed  CAS  Google Scholar 

  • Brady RA, O’May GA, Leid JG et al (2011) Resolution of Staphylococcus aureus biofilm infection using vaccination and antibiotic treatment. Infect Immun 79:1797–1803

    Article  PubMed  CAS  Google Scholar 

  • Broughan J, Anderson R, Anderson AS (2011) Strategies for and advances in the development of Staphylococcus aureus prophylactic vaccines. Expert Rev Vaccines 10:695–708

    Article  PubMed  CAS  Google Scholar 

  • Chan WC, Coyle BJ, Williams P (2004) Virulence regulation and quorum sensing in staphylococcal infections: competitive AgrC antagonists as quorum sensing inhibitors. J Med Chem 47:4633–4641

    Article  PubMed  CAS  Google Scholar 

  • Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM (1995) Microbial biofilms. Annu Rev Microbiol 49:711–745

    Article  PubMed  CAS  Google Scholar 

  • Ferguson BJ, Stolz DB (2005) Demonstration of biofilm in human bacterial chronic rhinosinusitis. Am J Rhinol 19:452–457

    PubMed  Google Scholar 

  • Fong WK, Modrusan Z, McNevin JP et al (2000) Rapid solid-phase immunoassay for detection of methicillin-resistant Staphylococcus aureus using cycling probe technology. J Clin Microbiol 38:2525–2529

    PubMed  CAS  Google Scholar 

  • Foster TJ (2005) Immune evasion by staphylococci. Nat Rev Microbiol 3:948–958

    Article  PubMed  CAS  Google Scholar 

  • Gjodsbol K, Christensen JJ, Karlsmark T et al (2006) Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J 3:225–231

    Article  PubMed  Google Scholar 

  • Hansson C, Hoborn J, Möller A, Swanbeck G (1995) The microbial flora in venous leg ulcers without clinical signs of infection. Repeated culture using a validated standardised microbiological technique. Acta Derm Venereol 75:24–30

    PubMed  CAS  Google Scholar 

  • Harro JM, Peters BM, O’May GA et al (2010) Vaccine development in Staphylococcus aureus: taking the biofilm phenotype into consideration. FEMS Immunol Med Microbiol 59:306–323

    PubMed  CAS  Google Scholar 

  • Hussain M, Wilcox MH, White PJ (1993) The slime of coagulase-negative staphylococci: biochemistry and relation to adherence. FEMS Microbiol Rev 10:191–207

    PubMed  CAS  Google Scholar 

  • Klein E, Smith DL, Laxminarayan R (2007) Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. Emerg Infect Dis 13:1840–1846

    Article  PubMed  Google Scholar 

  • Kluytmans J, van Belkum A, Verbrugh H (1997) Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 10:505–520

    PubMed  CAS  Google Scholar 

  • Lederer SR, Riedelsdorf G, Schiffl H (2007) Nasal carriage of methicillin resistant Staphylococcus aureus: the prevalence, patients at risk and the effect of elimination on outcomes among outclinic haemodialysis patients. Eur J Med Res 12:284–288

    PubMed  CAS  Google Scholar 

  • Leid JG, Shirtliff ME, Costerton JW, Stoodley P (2002) Human leukocytes adhere to, penetrate, and respond to Staphylococcus aureus biofilms. Infect Immun 70:6339–6345

    Article  PubMed  CAS  Google Scholar 

  • Lew DP, Waldvogel FA (2004) Osteomyelitis. Lancet 364:369–379

    Article  PubMed  CAS  Google Scholar 

  • Middleton JR (2008) Staphylococcus aureus antigens and challenges in vaccine development. Expert Rev Vaccines 7:805–815

    Article  PubMed  CAS  Google Scholar 

  • Morell EA, Balkin DM (2010) Methicillin-resistant Staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial development. Yale J Biol Med 83:223–233

    PubMed  CAS  Google Scholar 

  • National Nosocomial Infections Surveillance (1999) National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1990–May 1999, issued June 1999. Am J Infect Control 27:520–532

    Article  Google Scholar 

  • Noskin GA, Rubin RJ, Schentag JJ et al (2008) Budget impact analysis of rapid screening for Staphylococcus aureus colonization among patients undergoing elective surgery in US hospitals. Infect Control Hosp Epidemiol 29:16–24

    Article  PubMed  Google Scholar 

  • Novick RP, Geisinger E (2008) Quorum sensing in staphylococci. Annu Rev Genet 42:541–564

    Article  PubMed  CAS  Google Scholar 

  • Prabhakara R, Harro JM, Leid JG, Harris M, Shirtliff ME (2011a) Murine immune response to a chronic Staphylococcus aureus biofilm infection. Infect Immun 79:1789–1796

    Article  PubMed  CAS  Google Scholar 

  • Prabhakara R, Harro JM, Leid JG et al (2011b) Suppression of the inflammatory immune response prevents the development of chronic biofilm infection due to methicillin resistant Staphylococcus aureus. Infect Immun 79:5010–5018

    Article  PubMed  CAS  Google Scholar 

  • Resch A, Leicht S, Saric M et al (2006) Comparative proteome analysis of Staphylococcus aureus biofilm and planktonic cells and correlation with transcriptome profiling. Proteomics 6:1867–1877

    Article  PubMed  CAS  Google Scholar 

  • Rothfork JM, Dessus-Babus S, Van Wamel WJ, Cheung AL, Gresham HD (2003) Fibrinogen depletion attenuates Staphylococcus aureus infection by preventing density-dependent virulence gene up-regulation. J Immunol 171:5389–5395

    PubMed  CAS  Google Scholar 

  • Rubin RJ, Harrington CA, Poon A et al (1999) The economic impact of Staphylococcus aureus infection in New York City hospitals. Emerg Infect Dis 5:9–17

    Article  PubMed  CAS  Google Scholar 

  • Sauer K, Camper AK, Ehrlich GD, Costerton JW, Davies DG (2002) Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol 184:1140–1154

    Article  PubMed  CAS  Google Scholar 

  • Stephenson MF, Mfuna L, Dowd SE et al (2010) Molecular characterization of the polymicrobial flora in chronic rhinosinusitis. J Otolaryngol Head Neck Surg 39:182–187

    PubMed  Google Scholar 

  • Stranger-Jones YK, Bae T, Schneewind O (2006) Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Natl Acad Sci USA 103:16942–16947

    Article  PubMed  CAS  Google Scholar 

  • Thomas D, Chou S, Dauwalder O, Lina G (2007) Diversity in Staphylococcus aureus enterotoxins. Chem Immunol Allergy 93:24–41

    Article  PubMed  CAS  Google Scholar 

  • Wenzel RP, Edmond MB (2001) The impact of hospital-acquired bloodstream infections. Emerg Infect Dis 7:174–177

    Article  PubMed  CAS  Google Scholar 

  • Wertheim HF, Melles DC, Vos MC et al (2005) The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 5:751–762

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA

    Nathan K. Archer & Mark E. Shirtliff

  2. Graduate Program in Life Sciences, Microbiology and Immunology Program, University of Maryland, Baltimore, MD, USA

    Nathan K. Archer

  3. Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA, USA

    J. William Costerton

  4. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA

    Jeff G. Leid

  5. Department of Microbiology and Immunology, Medical School, University of Maryland, Baltimore, MD, USA

    Mark E. Shirtliff

Authors
  1. Nathan K. Archer
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  2. J. William Costerton
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  3. Jeff G. Leid
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  4. Mark E. Shirtliff
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Corresponding author

Correspondence to Mark E. Shirtliff .

Editor information

Editors and Affiliations

  1. Center for Genomic Sciences, Allegheny-Singer Research Inst., East North Ave. 320, Pittsburgh, 15212-4772, Pennsylvania, USA

    Garth D. Ehrlich

  2. , Department of Orthopedic Surgery, Allegheny General Hospital, East North Avenue, Pittsburgh, 15212, USA

    Patrick J. DeMeo

  3. Center for Genomic Sciences, Biofilm Research, Allegheny-Singer Research Institute, East North Avenue 320, Pittsburgh, 15212, Pennsylvania, USA

    J. William Costerton

  4. Privatklinik Döbling GmbH, Heiligenstädter Str. 57-63, Wien, 1190, Austria

    Heinz Winkler

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© 2012 Springer-Verlag Berlin Heidelberg

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Archer, N.K., Costerton, J.W., Leid, J.G., Shirtliff, M.E. (2012). Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention. In: Ehrlich, G., DeMeo, P., Costerton, J., Winkler, H. (eds) Culture Negative Orthopedic Biofilm Infections. Springer Series on Biofilms, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29554-6_5

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