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Plant Response to Stress: Microelectrode Voltage-Clamp Studies

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Abstract

Microelectrode voltage-clamp techniques have existed since more than 60 years with the pioneering work on squid axons. In the past decades, the patch-clamp technique became the most widely used voltage-clamp technique for recording macroscopic currents in plant cells and it largely improved the descriptions of the roles and properties of various ion transport systems. However, the necessary removal of the cell wall limits its application to protoplasts. Microelectrode voltage-clamp techniques allow circumventing this problem in working with cells with their intact walls. With these techniques, whole cell currents could be recorded from electrically isolated plant cell types challenged by various environmental stresses that could not be addressed with the patch-clamp technique. In this chapter, we discuss findings concerning different types of ion channel regulations recorded using microelectrode techniques on cells upon challenge by abiotic or biotic stresses; sustained ion channel activities leading to osmotic changes and transient ion channel regulations possibly involved in the signaling process. We further highlight the complex regulations of ion channels observed in host and nonhost cells by harpins from plant pathogenic bacteria.

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Authors and Affiliations

  1. Laboratoire d’Electrophysiologie des Membranes, Institut de Biologie des Plantes, Université Paris Diderot-Paris 7, Sorbonne Paris Cité, 91405, Orsay, France

    François Bouteau & Daniel Tran

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  1. François Bouteau
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  2. Daniel Tran
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Correspondence to François Bouteau .

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  1. , Department of Chemistry, Oakwood University, Adventist Blvd. 7000, Huntsville, 35896, Alabama, USA

    Alexander G. Volkov

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Bouteau, F., Tran, D. (2012). Plant Response to Stress: Microelectrode Voltage-Clamp Studies. In: Volkov, A. (eds) Plant Electrophysiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29119-7_3

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