Abstract
Many chemical compounds have been isolated from different parts of B. ceiba worldwide. These belong mostly to phenolics, flavonoids, sesquiterpenoids, steroids, naphthoquinones and neolignans. Totally 16 compounds have been isolated from root, 8 from root bark, 3 from stem bark, 3 from heart wood, 2 from leaves, 78 from flowers, 19 from seeds and 11 from gum. Several of these compounds are novel and isolated for the first time. Compounds isolated from this plant possess very important biological activities including immunomodulatory, cytotoxic, antineoplastic, anti-inflammatory, hypotensive, hypolipidemic, antihyperglycemic and antioxidant.
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References
Aragão GF (2004) Antiinflammatory, antiaggregant activity and central effects of alpha and beta amyrin from Protium heptaphyllum Aubl March. http://www.openthesis.org/documents/Antiinflammatory-antiaggregant-activity-central-effects-332976.html. Accessed 2 Nov 2011
Aragao GF, Pinheiro MCC, Bandeira PN, Lemos TLG, Viana GSB (2008) Analgesic and Anti-Inflammatory Activities of the Isomeric Mixture of Alpha- and Beta-Amyrin from Protium heptaphyllum(Aubl.) March. J Herbal Pharmacother 7(2):31–47
Balakrishnan A, Menon VP (2007) Antioxidant properties of hesperidin in nicotine-induced lung toxicity. Fundam Clin Pharmacol 21(5):535–546
Balanehru S, Nagarajan B (1991) Protective effect of oleanolic acid and ursolic acid against lipid peroxidation. Biochem Int 24(5):981–990
Baskar AA, Ignacimuthu S, Paulraj GM, Numair KL (2010) Chemopreventive potential of β-Sitosterol in experimental colon cancer model—an In vitro and In vivo study. BMC Complement Alternat Med 10:24. doi:10.1186/1472-6882-10-24
Bhatnagar MS (1965) Chemical composition of calyx of Salmalia malabarica. Sci Cult 31:189
Biswas M, Biswas K, Ghosh AK, Haldar PK (2009) A pentacyclic triterpenoid possessing analgesic activity from the fruits of Dregea volubilis. Phcog Mag 5:90–92
Boonsri S, Karalai C, Ponglimanont C, Chantrapromma S, Kanjana-opas A (2008) Cytotoxic and antibacterial sesquiterpenes from Thespesia populnea. J Nat Prod 71:1173–1177
Borg J (1991) The neurotrophic factor, n-hexacosanol, reduces the neuronal damage induced by the neurotoxin, kainic acid. J Neurosci Res 29(1):62–67
Bose S, Dutta AS (1963a) Structure of Salmalia malabarica gum. I. Nature of sugars present and the structure of aldobiuronic acid. J Indian Chem Soc 40:257–262
Bose S, Dutta AS (1963b) The structure of Salmalia malabarica gum. II. Structure of the degraded gum. J Indian Chem Soc 40:557–561
Bose S, Dutta AS (1965) The structure of Bombax malabarica gum. III. A tentative structure from methylation studies. J Indian Chem Soc 42:367–372
Broker R, Bhat JV (1953) Riboflavin and thiamine contents of gums. Curr Sci 22:343
Chadha YR (1972) The Wealth of India, raw material, vol IX. Publications and Information Directorate, New Delhi
Chauhan JS, Sultan M, Srivastav SK (1980) Constituents from Salmalia malabaricum. Can J Chem 58:328–330
Chen D, Landis-Piwowar KR, Chen MS, Dou QP (2007) Inhibition of proteasome activity by the dietary flavonoid apigenin is associated with growth inhibition in cultured breast cancer cells and xenografts. Breast Cancer Res 9(6):R80
Chen G, Bower KA, Xu M, Ding M, Shi X, Ke Z-J, Luo J (2009) Cyanidin-3-glucoside reverses ethanol-induced inhibition of neurite outgrowth: role of glycogen synthase kinase 3 beta. Neurotoxic Res 15:321–331
Chiba H, Uehara M, Wu J, Wang X, Masuyama R, Suzuki K, Kanazawa K, Ishimi Y (2003) Hesperidin, a Citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice. J Nutr 133(6):1892–1897
Choi S, Kim KW, Choi JS, Han ST, Park YI, Lee SK, Kim JS, Chung MH (2002) Angiogenic activity of beta-sitosterol in the ischaemia/reperfusion-damaged brain of Mongolian gerbil. Planta Med 68(4):330–335
Damgé C, Hillaire-Buys D, Koenig M, Gross R, Hoeltzel A, Chapal J, Balboni G, Borg J, Ribes G (1995) Effect of n-hexacosanol on insulin secretion in the rat. Eur J Pharmacol 274(1–3):133–139
Dar A, Faizi S, Naqvi S, Roome T, Zikr-ur-Rehman S, Ali M, Firdous S, Moin ST (2005) Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol Pharm Bull 28(4):596–600
Deharo E, Sauvain M, Moretti C, Richard B, Ruiz E, Massiot G (1992) Antimalarial effect of n-hentriacontanol isolated from Cuatresia sp (Solanaceae). Ann Parasitol Hum Comp 67(4):126–127
Dhar DN, Munjal RC (1976) Chemical examination of the seeds of Bombax malabaricum. Planta Med 29:148–150
Dhingra D, Chhillar R, Gupta A (2011) Antianxiety-like activity of gallic acid in instressed and stressed Mice: possible involvement of nitriergic system. Neurochem Res doi:10.1007/S11064-011-0635-7(EPub)
El-Hagrassi AM, Ali MM, Osman AF, Shaaban M (2011) Phytochemical investigation and biological studies of Bombax malabaricum flowers. Nat Prod Res 25(2):141–151
Faizi S, Ali M (1999) Shamimin: a new flavonol C-glycoside from leaves of Bombax ceiba. Planta Med 65(4):383–385
Fan P, Hay A-E, Marston A, Hostettmann K (2008) Acetylcholinesterase-inhibitory activity of Linarin from Buddleja davidii. Structure-activity relationships of related flavonoids, and chemical investigation of Buddleja nitida. Pharmaceutical Biol 46(9):596–601
Fernández S, Wasowski C, Paladini AC, Marder M (2004) Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol Biochem Behav 77(2):399–404
Field FJ, Born E, Mathur SN (1997) Effect of micellar beta-sitosterol on cholesterol metabolism in CaCo-2 cells. J Lipid Res 38:348–360
Fuhrman B, Lavy A, Aviram M (1995) Consumption of red wine with meals reduces the susceptibility of human plasma and low-density lipoproteins to lipid peroxidation. Am Soc Clin Nutr 61:549–554
Ghate VS, Agte VV, Vartak VD (1988) Promising economic potential of Shemul Bombax ceiba L. as a tuber crop. Indian J Forestry 11:158–159
Ghose TP (1935) Bombax malabaricum. Indian Forester 61:93–103
Gopal H, Gupta RK (1972) Chemical constituents of Salmalia malabarica Schott & Endl. Flowers. J Pharma Sci 61:807–808
Grael CFF, Albuquerque S, Lopes JLC (2005) Chemical constituents of Lychnophora pohlii and trypanocidal activity of crude plant extracts and of isolated compounds. Fitoterapia 76:73–82
Habtemariam S (2011) α-glucosidase inhibitory activity of kaempferol-3-O-rutinoside. Nat Prod Commun 6(2):201–203
Hibasami H, Saitoh K, Katsuzaki H, Imai K, Aratanechemuge Y, Komiya T (2004) 2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells. Int J Mol Med 14(6):1029–1033
Hoffmann-Bohm K, Lotter H, Seligmann O, Wagner H (1992) Antihepatotoxic C-glycosylflavones from the leaves of Allophyllus edulis var. edulis and gracilis. Planta Med 58:544–548
Hossain E, Mandal SC, Gupta JK (2011) Phytochemical Screening and in vivo antipyretic activity of the methanol leaf-extract of Bombax malabaricum DC (Bombacaceae). Trop J Pharm Res 10(1):55–60
Igarashi K, Ohmuna M (1995) Effect of Isoharnneti, Rhamnetin and Quercetin on the concentrations of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities in rats. Biosci Biotech Biochem 59:595–601
Itoigawa M, Takeya K, Ito C, FuruKawa H (1999) Structure activity relationship of cardiotonic flavonoids in guinea pig papillary muscle. J Ethnopharmacol 65:267–272
Ivorra MD, Docon MP, Paya M, Villar A (1988) Antihyperglycemic and insulin-releasing effects of beta-sitosterol-3-beta-d-glucoside and its aglycone, beta-sitosterol. Arch Int Pharmacodyn Ther 296:224–231
Izzo AA, Carlo GD, Mascolo N, Capasso F, Autore G (1994) Effects of quercetin on gastrointestinal tract: further studies. Phytother Res 8:179–185
Jahaniani F, Ebrahimi SA, Rahbar-Roshandel N, Mahmoudian M (2005) Xanthomicrol is the main cytotoxic component of Dracocephalum kotschyii and a potential anti-cancer agent. Phytochemistry 66(13):1581–1592
Jain V, Verma SK, Katewa SS, Anandjiwala S, Singh B, 4 (2011) Free radical scavenging property of Bombax ceiba L. root. Res J Med Plant 5(4):462–470
Jeong H-J, Choi Y, Kim K-Y, Kim M-H, Kim H-M (2011) C-Kit binding properties of Hesperidin (a Major Component of KMP6) as a potential anti-allergic agent. PLoS ONE 6(4):19528. doi:10.1371/journal.pone.0019528
Kapoor VP, Khan PSH, Raina RM, Farooqi MIH (1975) Chemical analysis of seeds: Part III from 40 non-leguminous species. Sci Cult 41:336–339
Kim MJ, Seong AR, Yoo JY, Jin CH, Lee YH, Kim YJ, Lee J, Jun WJ, Yoon HG (2011a) Gallic acid, a histone acetyltransferase inhibitor, suppresses β-amyloid neurotoxicity by inhibiting microglial-mediated neuroinflammation. Mol Nutr Food Res. doi:10.1002/mnfr.201100262
Kim SJ, Chung WS, Kim SS, Ko SG, Um JY (2011b) Antiinflammatory effect of Oldenlandia diffusa and its constituent, hentriacontane, through suppression of caspase-1 activation in mouse peritoneal macrophages. Phytother Res 25(10):1537–1546
Koo H, Pearson SK, Scott-Anne K, Abranches J, Cury JA, Rosalen PL, Park YK, Marquis RE, Bowen WH (2002) Effects of apigenin and tt-farnesol on glucosyltransferase activity, biofilm viability and caries development in rats. Oral Microbiol Immunol 17(6):337–343
Kroes BH, van den Berg AJ, Quarles van Ufford HC, van Dijk H, Labadie RP (1992) Anti-inflammatory activity of gallic acid. Planta Med 58(6):499–504
Krogh R, Yunes RA, Andricopulo AD (2000) Structure–activity relationships for the analgesic activity of gallic acid derivatives. Il Farmaco 55(11–12):730–735
Lagnika L, Wenigerb B, Vonthron-Senecheaub C, Sanni A (2009) Antiprotozoal activities of compounds isolated from Croton lobatus L. Afr J Infect Dis 3(1):1–5
Lee J-H, Lee JY, Park JH, Jung HS, Kim JS, Kang SS, Kim YS, Han Y (2007) Immunoregulatory activity by daucosterol, a β-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice. Vaccine 25(19):3834–3840. doi:10.1016/j.vaccine.2007.01.108
Lee KM, Lee KW, Jung SK, Lee EJ, Heo YS, Bode AM, Lubet RA, Lee HJ, Dong Z (2010) Kaempferol inhibits UVB-induced COX-2 expression by suppressing Src kinase activity. Biochem Pharmacol 80(12):2042–2049
Liu J (1995) Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol 49(2):57–68
Liu Z, Schwimer J, Liu D, Lewis J, Greenway FL, York DA, Woltering EA (2006) Gallic acid is partially responsible for the antiangiogenic activities of Rubus leaf extract. Phytother Res 20(9):806–813
Loizou S, Lekakis I, Chrousos GP, Moutsatsou P (2010) Beta-sitosterol exhibits anti-inflammatory activity in human aortic endothelial cells. Mol Nutr Food Res 54(4):551–558
Lorget F, Prouillet C, Petit J-P, Fardelonne P, Brazier M, Wattel A, Kamel S, Mentaverri R (2003) Potent inhibitory effect of naturally occurring flavonoids quercetin and kaempferol on in vitro osteoclastic bone resorption. Biochem Pharmacol 65(1):35–42
Marrassini C, Davicino R, Acevedo C, Anesini C, Gorzalczany S, Ferraro G (2011) Vicenin-2, a potential anti-inflammatory constituent of Urtica circularis. J Nat Prod 74:1503–1507
Martínez-González J, Rodríguez-Rodríguez R, González-Díez M, Rodríguez C, Herrera MD, Ruiz-Gutierrez V, Badimon L (2008) Oleanolic acid induces prostacyclin release in human vascular smooth muscle cells through a cyclooxygenase-2-dependent mechanism. J Nutr 138:443–448
McAnlis GT, Mc Enany J, Pearce J, Young IY (1997) The effect of various dietary flavonoids on the susceptibility of low-density lipoproteins to oxidation in vitro using both metallic and nonmetallic oxidising agents. Biochem Soc Trans 25:142–148
Meckes M, Calzada F, Paz D, Rodríguez J, Ponce-Monter H (2002) Inhibitory effect of xanthomicrol and 3α-angeloyloxy-2α-hydroxy-13,14Z-dehydrocativic acid from Brickellia paniculata on the contractility of guinea-pig Ileum. Planta Med 68(5):467–469
Min YS, Yim SH, Bai KL, Choi HJ, Jeong JH, Song HJ, Park SY, Ham I, Whang WK, Sohn UD (2005) The effects of apigenin-7-O-beta-d-glucuronopyranoside on reflux oesophagitis and gastritis in rats. Auton Autacoid Pharmacol 25(3):85–91
Monforte MT, Trovato A, Kirjavainen S, Forestieri AM, Galati EM, Lo Curto RB (1995) Biological effects of hesperidin, a Citrus flavonoid. (note II): hypolipidemic activity on experimental hypercholesterolemia in rat. Farmaco 50(9):595–599
Mukherjee J, Roy B (1971) Chemical examination of Salmalia malabarica Schott & Endl syn Bombax malabaricum DC. J Indian Chem Soc 48:769–770
Muruganandan S, Srinivasan K, Gupta S, Gupta PK, Lal J (2005) Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. J Ethnopharmacol 97:497–501
Nagaprashantha LD, Vatsyayan R, Singhal J, Fast S, Roby R, Awasthi S, Singhal SS (2011) Anti-cancer effects of novel flavonoid vicenin-2 as a single agent and in synergistic combination with docetaxel in prostate cancer. Biochem Pharmacol 82(9):1100–1109
Nagarathnam D, Cushman M (1991) A short and facile synthetic route to hydroxylated flavones. New syntheses of apigenin, tricin, and luteolin. J Org Chem 56:4884–4887
Niranjan GS, Gupta PC (1973) Anthocyanins from flowers of Bombax malabaricum. Planta Med 24(2):196–199
Okada S, Saito M, Kazuyama E, Hanada T, Kawaba Y, Hayashi A, Satoh K, Kanzaki S (2008) Effects of N-hexacosanol on nitric oxide synthase system in diabetic rat nephropathy. Mol Cell Biochem 315(1–2):169–177
Okoko T, Oruambo IF (2009) Inhibitory activity of quercetin and its metabolite on lipopolysaccharide-induced activation of macrophage U937 cells. Food Chem Toxicol 47(4):809–812
Online document 1. Chapter 8. Antibacterial, antioxidant and cytotoxic activity of taraxerol, a petacyclic triterpenoid, isolated from Pteleopsis myrtifolia leaves. http://upetd.up.ac.za/thesis/available/etd-05082008-131034/unrestricted/08chapter8.pdf. Accessed 4 Nov 2011
Puckhaber LS, Stipanovic RD (2001) Revised structure for a Sesquiterpene lactone from Bombax malabaricum. J Nat Prod 64(2):260–261
Qi Y, Guo S, Xia Z, Xie D (1996) Chemical constituents of Gossampinus malabarica (L.) Merr. (II). Zhongguo Zhong Yao Za Zhi 21(4):234–235, 256
Rajnarayana K, Reddy MS, Chaluvadi MR, Krishna DR (2001) Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol 33:2–16
Rajput MS, Mathur V, Agrawal P, Chandrawanshi HK, Pilaniya U (2011) Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley. Nat Prod Res. [Epub ahead of print]
Rastogi RP, Mehrotra BN (1995) Compendium of Indian medicinal plants, vol 4. PID, New Delhi
Reddy MVB, Reddy MK, Duvvuru G, Murthy MM, Caux C, Bodo B (2003) A new Sesquiterpene lactone from Bombax malabaricum. Chem Pharm Bull 51(4):458–459
Rizvi SAI, Saxena OC (1974) New glycosides, terpenoids, colouring matters, sugars and fatty compounds from the flowers of Salmalia malabarica. Arzneim-Forsch 24(3):285–287
Robak J, Glyglewski RJ (1988) Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37:837–841
Said A, Aboutable EA, Nofal SM, Tokuda H, Raslan M (2011) Phytoconstituents and bioactivity evaluation of Bombax ceiba L. flowers. J Trad Med 28:55–62
Saleem R, Ahmad M, Hussain SA, Qazi AM, Ahmad SI, Qazi MH, Ali M, Faizi S, Akhtar S, Hussain SN (1999) Hypotensive, hypoglycaemic and toxicological studies on the Flavonol C-glucoside Shamimin from Bombax ceiba. Planta Med 65:331–334
Saleem R, Ahmad SI, Ahmad M, Faizi Z, Rehman S, Ali M, Faizi S (2003) Hypotensive activity and toxicology of constituents from Bombax ceiba stem bark. Biol Pharm Bull 26(1):41–46
Sanchez De RVR, Somoza B, Ortega T, Villar A (1996) Flavonoids action on heart. Planta Med 62:554–562
Sankaram AVB, Reddy NS, Shoolery JN (1981) New sesquiterpenoids of Bombax malabaricum. Phytochemistry 20:1877–1881
Sengupta S, Mukherjee A, Goswami R, Basu S (2009) Hypoglycemic activity of the antioxidant saponarin, characterized as alpha-glucosidase inhibitor present in Tinospora cordifolia. J Enzyme Inhib Med Chem 24(3):684–690
Seshadri V, Batta AK, Rangaswami S (1971) Phenolic components of Bombax malabaricum (Root-Bark). Curr Sci 23:630
Shahat AA, Hassan RA, Nazif NM, Van Miert S, Pieters L, Hammuda FM, Vlietinck AJ (2003) Isolation of mangiferin from Bombax malabaricum and structure revision of shamimin. Planta Med 69(11):1068–1070
Shinbori C, Saito M, Kinoshita Y, Satoh I, Kono T, Hanada T, Nanba E, Adachi K, Suzuki H, Yamada M, Satoh K (2006) N-hexacosanol reverses diabetic induced muscarinic hypercontractility of ileum in the rat. European J Pharmacol 545(2–3):177–184
Shinha H, Ki-hyun S, Dongsool Y, Sookyeon L, Chong-kil L, Nam-ju H, Kyungjae K (2002) The effect of linarin on LPS-induced cytokine production and nitric oxide inhibition in murine macrophages cell line RAW264.7. Arch Pharm Res 25(2):170–177
Singh AB, Yadav DK, Maurya R, Srivastava AK (2009) Antihyperglycaemic activity of alpha-amyrin acetate in rats and db/db mice. Nat Prod Res 23(9):876–882
Sood RP, Suri KA, Suri OP, Dhar KL, Atal CK (1982) Sesquiterpene lactone from Salmalia malabarica. Phytochemistry 21(8):2125–2126
Sreeramulu K, Rao KV, Venkatarao C, Gunasekar D (2001) A new naphthoquinone from Bombax malabaricum. J Asian Nat Prod Res 3:261–265
Stoilova I, Jirovetz L, Stoyanova A, Krastanov A, Gargova S, Ho L (2008) Antioxidant activity of the polyphenol mangiferin. EJEAFChe 7(13):2706–2716
Subarnas A, Tadano T, Nakahata N, Arai Y, Kinemuchi H, Oshima Y, Kisara K, Ohizumi Y (1993) A possible mechanism of antidepressant activity of beta-amyrin palmitate isolated from Lobelia inflata leaves in the forced swimming test. Life Sci 52(3):289–296
Szakiel A, Ruszkowski D, Grudniak A, Kurek A, Wolska KI, Doligalska M, Janiszowska W (2008) Antibacterial and antiparasitic activity of oleanolic acid and its glycosides isolated from marigold (Calendula officinalis). Planta Med 74(14):1709–1715
Tang R, Chen K, Cosentino M, Lee KH (1994) Apigenin-7-O-β-d-glucopyranoside, an anti-HIV principle from Kummerowia striata Bioorganic & Medicinal Chemistry Letters 4(3):455–458
Velozo LSM, Ferreira MJP, Santos MIS, Moreira DL, Guimaraes EF, Emerenciano VP, Kaplan MAC (2009) C-glycosyl flavones from Peperomia blanda. Fitoterapia 80:119–122
Villaseñor IM, Angelada J, Canlas AP, Echegoyen D (2002) Bioactivity studies on beta-sitosterol and its glucoside. Phytother Res 16(5):417–421
Vitcheva V, Simeonova R, Krasteva I, Yotova M, Nikolov S, Mitcheva M (2011) Hepatoprotective effects of saponarin, isolated from Gypsophila trichotoma Wend. on cocaine-induced oxidative stress in rats. Redox Rep 16(2):56–61
Wal P, Wal A, Sharma G, Rai AK (2011) Biological activities of lupeol. Syst Rev Pharm 2:96–103
Wang H, Zeng Z, Zeng H-P (2003) Study on chemical constituents of petroleum ether fraction of alcohol extract from the flower of Bombax malabaricum. Chem Ind Forest Products. doi:cnki:issn:0253-2417.0.2003-01-018
Wang R-R, Gao Y-D, Ma C-H, Zhang X-J, Huang C-G, Huang J-F, Zheng Y-T (2011) Mangiferin, an Anti-HIV-1 agent targeting protease and effective against resistant strains. Molecules 16:4264–4277
Wu J, Zhang XH, Zhang SW, Xuan LJ (2008) Three novel compounds from the flowers of Bombax malabaricum. Helvetica Chimica Acta 91(1):136–143
Yin MC, Chan KC (2007) Nonenzymatic antioxidative and anti-glycative effects of oleanolic acid and ursolic acid. J Agric Food Chem 55(17):7177–7181
You YJ, Nam NH, Kim HM, Bae KH, Ahn BZ (2003) Antiangiogenic activity of lupeol from Bombax ceiba. Phytother Res 17(4):341–344
Zhang X, Zhu H, Zhang S, Yu Q, Xuan L (2007) Sesquiterpenoids from Bombax malabaricum. J Nat Prod 70:1526–1528
Zoechling WA, Reiter E, Eder R, Wendelin S, Liebner F, Jungbauer A (2009) The flavonoid kaempferol is responsible for the majority of estrogenic activity in Red Wine. AJEV 60(2):223–232
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Jain, V., Verma, S.K. (2012). Phytochemical Studies. In: Pharmacology of Bombax ceiba Linn.. SpringerBriefs in Pharmacology and Toxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27904-1_3
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