Abstract
In the framework of an interdisciplinary research devoted at increasing soil capacity to act as carbon sink by means of innovative and sustainable strategies (the MESCOSAGR Project), we studied, in microcosm-scale model systems, changes of selected soil chemical properties, soil CO2 efflux, and root morpho-topology after addition of either mature compost or a biomimetic catalyst (CAT) (synthetic water-soluble iron–porphyrin), as single addition or in combination of the two treatments. Direct effects of CAT on seed germination, seedling establishment, and plant growth were also evaluated in model plant species. When applied to bare soil, CAT was able to reduce CO2 emission from soil. Soil amendment of compost alone stimulated CO2 emission from soil, whereas its combined addition with CAT strongly depressed the compost-induced CO2 release. In planted microcosms, the contribution of the rhizosphere-derived CO2 efflux markedly increased the total soil respiration and CAT addition further stimulated CO2 release from soil. It is thus suggested that iron–porphyrin, growth of maize root, and CO2 release are functionally interconnected. The increased total soil respiration observed in planted systems may be due to a larger contribution of the rhizosphere-derived CO2 efflux, as a consequence of secondary actions or specific mutual interactions of the catalyst-root system. The direct CAT effect on model plant species implied a complex pattern of dose-dependent, and, remarkably, species-specific responses, as observed in both root systems and aerial plant parts. The observed strong CAT promotion of the synthesis of photosynthetic pigments might indicate an in planta uptake and translocation of the CAT molecule, prompting to envisage potential applications of this molecule in a wider agro-biotechnological context.
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Abbreviations
- CAT:
-
[meso-tetra(2,6-dichloro-3-sulfonatophenyl) porphyrinate of Fe(III)chloride]
- CEC:
-
Cation exchange capacity
- Chl:
-
Chlorophyll
- Chlide:
-
Chlorophyllide
- EC:
-
Electrical conductivity
- F:
-
Root fineness
- LR:
-
Root length
- MS:
-
Murashige and Skoog medium
- Pchlide:
-
Protochlorophyllide
- POR:
-
NADPH:protochlorophyllide oxidoreductase
- RMR:
-
Root mass ratio
- SOM:
-
Soil organic matter
- TD:
-
Tissue mass density
- TI:
-
Topological index
- TN:
-
Total nitrogen
- TOC:
-
Total organic carbon
- VR:
-
Root volume
- WP:
-
Plant dry weight
- WR:
-
Root dry weight
- WS:
-
Shoot dry weight
References
Armstrong GA, Apel K, Wolfhart R (2000) Does a light-harvesting protochlorophyllide a/b-binding protein complex exist? Trends Plant Sci 5:40–44
Bastida F, Kandeler E, Moreno JL, Garcia C, Hernandez T (2008) Application of fresh and composted organic wastes modifies structure, size and activity of soil microbial community under semiarid climate. Appl Soil Ecol 40:318–429
Borken W, Muhs A, Beese F (2002) Application of compost in spruce forest: effects on soil respiration, basal respiration and microbial biomass. For Ecol Manag 159:49–58
Cheng W-X (2008) Rhizosphere priming effect: its functional relationships with microbial turnover, evapotranspiration, and C–N budgets. Soil Biol Biochem 41:1795–1801
Cheng W, Kuzyakov Y (2005) Root effects on soil organic matter decomposition. In: Zobel RW, Wright SF (eds) Roots and soil management: interactions between the roots and the soil, Agronomy monograph 48. ASA, CSSA and SSSA, Madison, WI, pp 119–144
Clark LJ, Whalley WR, Barraclough PB (2003) How do roots penetrate strong soil? Plant Soil 255:93–104
Dijkstra FA, Cheng W-X (2007) Interactions between soil and tree roots accelerate long-term soil carbon decomposition. Ecol Lett 10:1046–1053
Eissenstat DM, Achor DS (1999) Anatomical characteristics of roots of citrus rootstocks that vary in specific root length. New Phytol 141:309–321
Ellert BH, Janzen HH (1999) Short-term influence of tillage on CO2 fluxes from a semi-arid soil on the Canadian Prairies. Soil Till Res 50:21–32
Fitter AH (1986) The topology and geometry of plant root system: influence of watering rate on root system topology in Trifolium pratense. Ann Bot 58:91–101
Fukushima M, Shigematsu S, Nagao S (2010) Influence of humic acid type on the oxidation products of pentachlorophenol using hybrid catalysts prepared by introducing iron(III)-5,10,15,20-tetrakis (p-hydroxyphenyl) porphyrin into hydroquinone-derived humic acids. Chemosphere 78:1155–1159
Gelsomino A, Tortorella D, Cianci V, Petrovičová B, Sorgonà A, Piccolo A, Abenavoli MR (2010) Effects of a biomimetic iron-porphyrin on soil respiration and maize root morphology as by a microcosm experiment. J Plant Nutr Soil Sci 173:399–406
Glimskär A (2000) Estimates of root system topology of five plant species grown at steady-state nutrition. Plant Soil 227:248–256
Hahn D, Cozzolino A, Piccolo A, Armenante PM (2007) Reduction of 2,4-dichlorophenol toxicity to Pseudomonas putida after oxidative incubation with humic substances and a biomimetic catalyst. Ecotoxicol Environ Saf 66:335–342
Haynes RJ, Beare MH (1997) Influence of six crop species on aggregate stability and some labile organic matter fractions. Soil Biol Biochem 29:1647–1653
Helal HM, Sauerbeck DR (1984) Influence of plant root on C and P metabolism in soil. Plant Soil 76:175–182
Helal HM, Sauerbeck DR (1986) Effect of plant roots on carbon metabolism of soil microbial biomass. Z Pflanzenernähr Bodemkd 149:181–188
Heyes DJ, Sakuma M, Scrutton NS (2007) Laser excitation studies of the product release steps in the catalytic cycle of the light-driven enzyme, protochlorophyllide oxidoreductase. J Biol Chem 282:32015–32020
Kampichler C, Bruckner A, Kandeler E (2001) Use of enclosed model ecosystems in soil ecology: a bias towards laboratory research. Soil Biol Biochem 32:269–275
Kuzyakov Y (2006) Sources of CO2 efflux from soil and review of partitioning methods. Soil Biol Biochem 38:425–448
Lal R (2007) Carbon management in agricultural soils. Mitig Adapt Strateg Glob Chang 12:303–322
Lesage S, Xu H, Durham L (1993) The occurrence and roles of porphyrins in the environment: possible implications for the bioremediation. Hydrolog Sci J 38:343–354
Lichtenthaler HK, Bach TJ, Wellburn AR (1982) Cytoplasmic and plastidic isoprenoid compounds of oat seedlings and their distinct labelling from 14C-mevalonate. In: Wintermans JFGM, Kuiper PJC (eds) Biochemistry and metabolism of plant lipids. Elsevier Biomedical, Amsterdam, pp 489–500
Lukkari T, Teno S, Väisänen A, Haimi J (2006) Effects of earthworms on decomposition and metal availability in contaminated soils: microcosms studies of populations with different exposure histories. Soil Biol Biochem 38:259–370
Moyano FE, Kutsch WL, Schulze E-D (2007) Response of mycorrhizal, rhizosphere and soil basal respiration to temperature and photosynthesis in a barley field. Soil Biol Biochem 39:843–853
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Piccolo A, Teshale AZ (1998) Soil processes and responses to climate changes. In: Peter D, Maracchi G, Ghazi A (eds) Climate change impact on agriculture and forestry. European Commission, Brussels, pp 79–92
Piccolo A, Conte P, Tagliatesta P (2005) Increased conformational rigidity of humic substances by oxidative biomimetic catalysis. Biomacromolecules 6:351–358
Piccolo A, Spaccini R, Nebbioso A, Mazzei P (2011) Soil carbon sequestration by in situ catalyzed photo-oxidative polymerization of soil organic matter. Environ Sci Technol. 45:6697–6702
Pregitzer KS, Zak DR, Maziasz J, DeForest J, Curtis PS, Lussenhop J (2000) Interactive effects of atmospheric CO2 and soil-N availability on fine roots of Populus tremuloides. Ecol Appl 10:18–33
Pregitzer KS, Burton AJ, King JS, Zak DR (2008) Soil respiration, root biomass, and root turnover following long-term exposure of northern forests to elevated atmospheric CO2 and tropospheric O3. New Phytol 180:152–161
Ryser P (1998) Intra- and interspecific variation in root length, root turnover and the underlying parameters. In: Lambers H, Poorter H, Van Vuuren MMI (eds) Inherent variation in plant growth: physiological mechanisms and ecological consequences. Backhuys, Leiden, pp 441–465
Ryser P, Lambers H (1995) Root and leaf attributes accounting for the performance of fast- and slow-growing grasses at different nutrient supply. Plant Soil 170:251–265
Sikora LJ, Stott DE (1996) Soil organic carbon and nitrogen. In: Doran JW, Jones AJ (eds) Methods for assessing soil quality. SSSA, Madison, WI, pp 157–167, SSSA Special Publication No. 49
Skribanek A, Apatini D, Inaoka M, Boddi B (2000) Protochlorophyllide and chlorophyll forms in dark-grown stems and stem-related organs. J Photochem Photobiol B: Biol 55:172–177
Smart DR, Peñuelas J (2005) Short-term CO2 emissions from planted soil subject to elevated CO2 and simulated precipitation. Appl Soil Ecol 28:247–257
Šmejkalová D, Piccolo A (2005) Enhanced molecular dimension of a humic acid induced by photooxidation catalysed by biomimetic metalporphyrins. Biomacromolecules 6:2120–2125
Šmejkalová D, Piccolo A, Spiteller M (2006) Oligomerization of humic phenolic monomers by oxidative coupling under biomimetic catalysis. Environ Sci Technol 40:6955–6962
Smith KM (1975) Porphyrins and metalloporphyrins. Elsevier, Amsterdam
Sparks DL (1996) Methods of soil analysis, part 3, chemical methods No. 5. SSSA, Madison
Tingey DT, Lee EH, Lewis JD, Johnson MG, Rygiewicz PT (2008) Do mesocosms influence photosynthesis and soil respiration? Env Exp Bot 62:36–44
Tortorella D, Gelsomino A (2011) Influence of compost amendment and maize root system on soil CO2 efflux: a mesocosm approach. Agrochimica LV 1:1–18
Traylor PS, Dolphin D, Traylor TG (1984) Sterically protected hemins with electronegative substituents: efficient catalysts for hydroxylation and epoxidation. J Chem Soc Chem Commun 1984:279–280
Tresder KK, Morris SJ, Allen MF (2005) The contribution of root exudates, symbionts, and detritus to carbon sequestration in the soil. In: Zobel RW, Wright SF (eds) Roots and soil management: interactions between the roots and the soil, Agronomy monograph 48. ASA, CSSA and SSSA, Madison, WI, pp 145–168
Wahl S, Ryser P (2000) Root tissue structure is linked to ecological strategies of grasses. New Phytol 148:459–471
Acknowledgments
The MESCOSAGR Project contributes to the Strategic Programme “Sustainable Development and Climate Changes”, funded through the Integrative Special Fund for Research by the Italian Ministry for Education, University and Research. The authors gratefully acknowledge the dedicated and competent scientific support from Demetrio Tortorella, Barbara Logoteta, Beatrix Petrovičová, and Giuseppe Princi. The technical assistance from Vincenzo Cianci was highly appreciated.
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Gelsomino, A., Panuccio, M.R., Sorgonà, A., Abenavoli, M.R., Badiani, M. (2012). Effects of Carbon Sequestration Methods on Soil Respiration and Root Systems in Microcosm Experiments and In Vitro Studies. In: Piccolo, A. (eds) Carbon Sequestration in Agricultural Soils. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23385-2_10
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DOI: https://doi.org/10.1007/978-3-642-23385-2_10
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