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Establishing an Animal Model of Dupuytren’s Contracture by Profiling Genes Associated with Fibrosis

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Book cover Dupuytren’s Disease and Related Hyperproliferative Disorders

Abstract

Dupuytren’s disease (DD) is characterized by the progressive development of a scar-like collagen-rich cord within the palmar fascia of the hand that results in permanent finger contracture. Currently, DD is most commonly treated by surgical resection of the diseased tissue. To date, no directly analogous animal model has been described in which to evaluate potential nonsurgical treatments for Dupuytren’s contracture. In the present study, we endeavor to establish an animal model that may ultimately serve as a platform in which to compare various treatments for DD. Hypothesizing that fibroblasts are the cellular effectors of the Dupuytren’s phenotype, we have transplanted fibroblasts derived from DD palmar fascia into the forepaw of nude rats. Our initial examinations showed that DD-derived fibroblasts persisted successfully in the forepaws of nude rats up to 56 days. Initial studies with quantitative real-time RT-PCR (qRT-PCR) using RNA derived from forepaw tissues harvested at 8 weeks showed that mRNA levels of alpha-smooth muscle actin (α-SMA) and type I collagen were significantly elevated in the forepaws of nude rats injected with DD-derived fibroblasts. The increase in α-SMA suggests an in vivo fibroblast to myofibroblast transformation that ultimately may result in scar formation and contracture.

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Acknowledgments

The authors thank Allegheny-Singer Research Institute, The Pittsburgh Foundation, and Pennsylvania Department of Health for their financial support toward this study. We also thank the staff of Lab Animal Resource Facility of ASRI for their support toward this research protocol.

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Authors and Affiliations

  1. Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA, USA

    Latha Satish, Sandra Johnson, J. Christopher Post, Garth D. Ehrlich & Sandeep Kathju

  2. Division of Upper Extremity Surgery, Department of Orthopaedics, Allegheny General Hospital, Pittsburgh, PA, USA

    Mark E. Baratz & Bradley Palmer

Authors
  1. Latha Satish
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  2. Mark E. Baratz
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  3. Bradley Palmer
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  4. Sandra Johnson
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  5. J. Christopher Post
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  6. Garth D. Ehrlich
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  7. Sandeep Kathju
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Corresponding author

Correspondence to Latha Satish .

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Editors and Affiliations

  1. The Hand Center, S Old Dixie Highway 1002, Jupiter, 33458, Florida, USA

    Charles Eaton

  2. Strahlenzentrum Hamburg, Langenhorner Chaussee 369, Hamburg, 22419, Germany

    M. Heinrich Seegenschmiedt

  3. Manchester Interdisciplinary Biocentre, Plastic & Reconstructive Surgery, University of Manchester, Princess Street 131, Manchester, M1 7DN, United Kingdom

    Ardeshir Bayat

  4. Faculty of Medicine, Dept. of Pathology and Immunology, University of Geneva, Rue Michel-Servet 1, Geneva, 1211, Switzerland

    Giulio Gabbiani

  5. Medical Center, Department of Plastic Surgery, University of Groningen, Hanzeplein 1, Groningen, 9713 GZ, Netherlands

    Paul Werker

  6. Dupuytren e.V., Westerbuchberg 60b, Übersee, 83236, Germany

    Wolfgang Wach

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© 2012 Springer-Verlag Berlin Heidelberg

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Satish, L. et al. (2012). Establishing an Animal Model of Dupuytren’s Contracture by Profiling Genes Associated with Fibrosis. In: Eaton, C., Seegenschmiedt, M., Bayat, A., Gabbiani, G., Werker, P., Wach, W. (eds) Dupuytren’s Disease and Related Hyperproliferative Disorders. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22697-7_13

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  • DOI: https://doi.org/10.1007/978-3-642-22697-7_13

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-22696-0

  • Online ISBN: 978-3-642-22697-7

  • eBook Packages: MedicineMedicine (R0)

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