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The Metallo-β-Lactamase Family of Ribonucleases

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Ribonucleases

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC))

Abstract

The metallo-β-lactamases (MβLs) constitute a very ancient family of enzymes with a wide range of substrates, including RNA and DNA. The β-lactamases that act on RNA (here called rMβLs) can be grouped into two major families, the RNase Z family and the β-CASP family. Members of the RNase Z family are primarily involved in the maturation of the 3′ end of tRNAs, whereas members of the β-CASP family have thus far been shown to have primarily mRNA and snRNA targets. Although they share a metallo-β-lactamase core and catalytic mechanism, the two families are easily distinguishable at the sequence level and by the presence of characteristic subdomains that play a key role in substrate recognition. These are principally the so-called flexible arm (about 40 amino-acids) of RNase Z and the β-CASP domain (>160 amino-acids) that gives its name to this family of enzymes. In this chapter, I will describe our current understanding of these two MβL families, with particular emphasis on their structures, their substrates, and their phylogenic distribution.

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Acknowledgments

This work was supported by funds from the CNRS (UPR 9073), Université Paris VII-Denis Diderot and the Agence Nationale de la Recherche (ANR- SubtilRNA2). I thank colleagues and current and former lab members for their contributions to the data discussed in this chapter. I also thank Béatrice Clouet-d’Orval, Zbigniew Dominski, and Allen Nicholson for their helpful comments on the manuscript.

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  1. CNRS UPR 9073 (affiliated with Université de Paris Diderot, Sorbonne Paris Cité), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005, Paris, France

    Ciarán Condon & Laetitia Gilet

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  1. Ciarán Condon
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  2. Laetitia Gilet
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Correspondence to Ciarán Condon .

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  1. Temple University, 1900 North 12th Street, Philadelphia, PA 19122, USA

    Allen W. Nicholson

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Condon, C., Gilet, L. (2011). The Metallo-β-Lactamase Family of Ribonucleases. In: Nicholson, A. (eds) Ribonucleases. Nucleic Acids and Molecular Biology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21078-5_10

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