Abstract
Reversible binding to one site can be calculated with analytical solutions. On the basis of these formulas, data can be transformed to appear linear. Straight lines of equilibrium-binding studies in double reciprocal plots or of enzyme kinetics in Lineweaver-Burk plots or of dissociation kinetics in half logarithmic plots indicate simple mechanisms. Deviations from these are discussed in detail as cooperative or independent sites. Logistic functions commonly used to calculate dose–response curves, only correspond to a binding mechanism when the Hill coefficient is one. At the end of this chapter, the reader should be able to identify simple biochemical reactions.
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Prinz, H. (2011). Classical Analytical Solutions. In: Numerical Methods for the Life Scientist. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20820-1_3
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DOI: https://doi.org/10.1007/978-3-642-20820-1_3
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