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Adipose Stem Cells and Regenerative Medicine pp 83–92Cite as

Storage of Adipose Stem Cells

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Abstract

Adipose-derived stem cells (ADSCs) are becoming the cells of choice for an increasing number of clinical trials, and they promise to be in the next decade the preferential cell type used in cell therapies. Here we describe how they can be isolated from adipose tissue, prepared for storage, and eventually conserved in liquid nitrogen, waiting for future cell therapy applications. In particular, we show how a mesenchymal stem cell bank could be structured for providing financial means to perform research and development related to cell therapies. But also the essentials of adipose tissue, ADSCs and how they can be characterized for in vitro growth, immuno-phenotyping and differentiation in other lineages like osteo-, chondro and adipo-. Freezing technology is also reviewed and results specific for patient-to-bench process are shown including legislative aspects allowing these cells to be used as a standardized transplant. Overall, these cells demonstrate that they can be easily extracted from adipose tissue, they can be grown in large amounts, differentiate in the three lineages, and possess the mesenchymal phenotype. Furthermore, they can resist very well to freezing/thawing procedures by maintaining a good capability of forming colonies (CFU-F).

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Authors and Affiliations

  1. Clinica Giorgio Bronz, Viale Castagnola 21f, 6900, Lugano, Switzerland

    Giorgio Bronz

  2. Swiss Stem Cell Foundation, Via Tesserete 48, 6900, Lugano, Switzerland

    Gianni Soldati

Authors
  1. Giorgio Bronz
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  2. Gianni Soldati
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Correspondence to Giorgio Bronz .

Editor information

Editors and Affiliations

  1. Plastic Surgery, Suchet 14 BD, Paris, 75016, France

    Yves-Gerard Illouz

  2. Regenerative Plastic Surgery Department, IASO General Hospital, Mesogeion Avenue 264, Athens, 15562, Greece

    Aris Sterodimas

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© 2011 Springer-Verlag Berlin Heidelberg

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Bronz, G., Soldati, G. (2011). Storage of Adipose Stem Cells. In: Illouz, YG., Sterodimas, A. (eds) Adipose Stem Cells and Regenerative Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20012-0_8

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  • DOI: https://doi.org/10.1007/978-3-642-20012-0_8

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-20011-3

  • Online ISBN: 978-3-642-20012-0

  • eBook Packages: MedicineMedicine (R0)

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