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Non Coding RNAs and Gene Silencing in Grape

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Non Coding RNAs in Plants

Part of the book series: RNA Technologies ((RNATECHN))

Abstract

Grapevine (Vitis vinifera L.) is a worldwide fruit crop of primary economic interest for berry consumption and winemaking. The molecular basis of grape berry ripening has been partially elucidated with the isolation and functional characterization of transcription factors which regulate sugar accumulation and secondary metabolism. After the recent publication of the complete sequence draft of two grapevine genotypes, a set of small non coding RNAs has been isolated by Sanger and high-throughput sequencing of small RNA libraries. These include conserved and grapevine-specific microRNAs as well as other small RNAs potentially involved in berry ripening. Small non coding RNAs are effectors of silencing pathways that underlie transgene silencing phenomena observed in several experiments of Agrobacterium-mediated transformation of grapevine. The knowledge of the silencing mechanisms in grapevine promises to facilitate the development of transient systems for gene functional studies.

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Acknowledgments

The authors thank Giampiero Valè (CRA-GPG Genomic Research Centre, Fiorenzuola d’Arda, Italy) for critical reading of the manuscript.

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Authors and Affiliations

  1. Research Unit for Wood Production Outside Forests, CRA, Strada Frassineto 35, 15033, Casale Monferrato, Italy

    Andrea Carra & Giuseppe Nervo

  2. Plant Virology Institute, Grugliasco Unit, CNR, Via L. da Vinci 44, 10095, Grugliasco, Italy

    Giorgio Gambino

  3. CRA-GPG Genomic Research Centre, Via S. Protaso 302, 29017, Fiorenzuola d’Arda, Italy

    Simona Urso

Authors
  1. Andrea Carra
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  2. Giorgio Gambino
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  3. Simona Urso
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  4. Giuseppe Nervo
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Corresponding author

Correspondence to Andrea Carra .

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Editors and Affiliations

  1. Institute of Chemistry/Biochemistry, Free University Berlin, Thielallee 63, Berlin, 14195, Berlin, Germany

    Volker A. Erdmann

  2. Inst. Bioorganic Chemistry, PAN Poznan, ul. Z. Noskowskiego 12/14, Poznan, 61-704, Poland

    Jan Barciszewski

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Carra, A., Gambino, G., Urso, S., Nervo, G. (2011). Non Coding RNAs and Gene Silencing in Grape. In: Erdmann, V., Barciszewski, J. (eds) Non Coding RNAs in Plants. RNA Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19454-2_5

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