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Coherent Light Microscopy pp 145–167Cite as

Quantitative Phase Imaging in Microscopy Using a Spatial Light Modulator

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Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 46))

Abstract

In this chapter, we present a new method capable of recovery of the quantitative phase information of microscopic samples. Essentially, a spatial light modulator (SLM) and digital image processing are the basics to extract the sample’s phase distribution. The SLM produces a set of misfocused images of the input sample at the CCD plane by displaying a set of lenses with different power at the SLM device. The recorded images are then numerically processed to retrieve phase information. Computations are based on the wave propagation equation and lead to a complex amplitude image containing information of both amplitude and phase distributions of the input sample diffracted wave front. The proposed configuration becomes a non-interferometric architecture (conventional transmission imaging mode) where no moving elements are included. Experimental results are provided in comparison with conventional digital holographic microscopy.

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Authors and Affiliations

  1. Departamento de Óptica, Universitat de Valencia, C/Doctor Moliner 50, 46100, Burjassot, Spain

    Vicente Micó

  2. Departamento de Óptica, Universitat de Valencia, 46100, Burjassot, Spain

    Javier García & Luis Camacho

  3. School of Engineering, Bar Ilan University, Ramat Gan, 52900, Israel

    Zeev Zalevsky

Authors
  1. Vicente Micó
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  2. Javier García
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  3. Luis Camacho
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  4. Zeev Zalevsky
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Correspondence to Vicente Micó .

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Editors and Affiliations

  1. Ist. Nazionale di Ottica Applicata, CNR, Via Campi Flegrei 34, Pozzuoli, 80078, Napoli, Italy

    Pietro Ferraro

  2. Dept. Biomedical Engineering, Duke University, Durham, 27708-0292, North Carolina, USA

    Adam Wax

  3. Dept. Engineering, Bar-Ilan University, Ramat Gan, 52900, Israel

    Zeev Zalevsky

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Micó, V., García, J., Camacho, L., Zalevsky, Z. (2011). Quantitative Phase Imaging in Microscopy Using a Spatial Light Modulator. In: Ferraro, P., Wax, A., Zalevsky, Z. (eds) Coherent Light Microscopy. Springer Series in Surface Sciences, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15813-1_6

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