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Second/Third Harmonic Generation Microscopy

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Abstract

When the energy density at the focal spot of a microscope is sufficiently large, nonlinear optical effects such as, harmonic generation, sum-frequency generation, coherent Raman scattering, parametric oscillations, and multi-photon fluorescence can be observed. These optical phenomena can be used in a nonlinear optical microscope to study the biological material. Nonlinear optical microscopy may be divided into incoherent and coherent modes. Incoherent nonlinear microscopy is characterized by the optical signal (like fluorescence) with a random phase, whose power is proportional to the concentration of radiating molecules.

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Authors and Affiliations

  1. Division of Bioengineering, National University of Singapore, 9 Engineering Drive, Singapore, 117576, Singapore

    Shakil Rehman, Naveen K. Balla, Elijah Y. Y. Seng & Colin J. R. Sheppard

  2. Singapore Eye Research Institute, 11, Third Hospital Avenue, #05-00, Singapore, 168751, Singapore

    Shakil Rehman

  3. Computation and Systems Biology, Singapore-MIT Alliance, National University of Singapore, Singapore, 117576, Singapore

    Naveen K. Balla

  4. Department of Diagnostic Radiology, National University of Singapore, 5 Lower Kent Ridge Road, Singapore, 119074, Singapore

    Colin J. R. Sheppard

Authors
  1. Shakil Rehman
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  2. Naveen K. Balla
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  3. Elijah Y. Y. Seng
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  4. Colin J. R. Sheppard
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Corresponding author

Correspondence to Shakil Rehman .

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Editors and Affiliations

  1. , Nanophysics Unit, The Italian Institute of Technology - II, Via Morego 30, Genova, 16163, Italy

    Alberto Diaspro

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Rehman, S., Balla, N.K., Seng, E.Y.Y., Sheppard, C.J.R. (2011). Second/Third Harmonic Generation Microscopy. In: Diaspro, A. (eds) Optical Fluorescence Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15175-0_3

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