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Low Concentration Protein Detection Using Novel SERS Devices

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Optical Fluorescence Microscopy

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Abstract

We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between –65°C and 90°C, using “Device1”, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. “Device2” is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.

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Authors and Affiliations

  1. Lab. BIONEM, Dipartimento di Medicina Sperimentale e Clinica, Università “Magna Graecia” di Catanzaro, Catanzaro, Italy

    Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco De Angelis, Patrizio Candeloro, Carlo Liberale & Enzo Di Fabrizio

  2. CalMED s.r.l., Catanzaro C.da Mula Loc. Germaneto, Campus Universitario Edificio Area Medicina, 88100, Catanzaro, Italy

    Gobind Das, Francesco Gentile, Maria Laura Coluccio, Federico Mecarini, Francesco De Angelis, Carlo Liberale & Enzo Di Fabrizio

  3. INFM–TASC–S.S., 163.5 Area Science Park, Basovizza, Trieste, Italy

    Gobind Das & Enzo Di Fabrizio

  4. Istituto Italiano di Tecnologia, via Morego 30, Genova, Italy

    Enzo Di Fabrizio

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  1. Gobind Das
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  2. Francesco Gentile
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  3. Maria Laura Coluccio
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  4. G. Cojoc
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  5. Federico Mecarini
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  6. Francesco De Angelis
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  7. Patrizio Candeloro
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  8. Carlo Liberale
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  9. Enzo Di Fabrizio
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Correspondence to Enzo Di Fabrizio .

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Editors and Affiliations

  1. , Nanophysics Unit, The Italian Institute of Technology - II, Via Morego 30, Genova, 16163, Italy

    Alberto Diaspro

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Das, G. et al. (2011). Low Concentration Protein Detection Using Novel SERS Devices. In: Diaspro, A. (eds) Optical Fluorescence Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15175-0_12

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