Skip to main content

Earthworms: A Potent Herbal Target for TCM (CAM) Research

  • Chapter
  • First Online:
Book cover Biology of Earthworms

Part of the book series: Soil Biology ((SOILBIOL,volume 24))

  • 3128 Accesses

Abstract

Earthworms, also known as Dilong, have been widely used in traditional Chinese medicine for thousands of years. The medicinal applications of earthworms in various remedies included blood stagnation removal, wound healing, epilepsy, asthma, hemiplegia, and rheumatism. Recently, evidence-based research has examined the therapeutic effects of earthworm and the current state of knowledge that earthworms have and the impact on and response to their environment. In this chapter, we discuss several observations: neurobiological mechanisms, with particular attention to nerve regeneration induced by earthworms, pharmacological functions, and impact on ecological systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Aizenman Y, de Vellis J (1987) Brain neurons develop in a serum and glial free environment: effects of transferrin, insulin, insulin-like growth factor-I and thyroid hormone on neuronal survival, growth and differentiation. Brain Res 406:32–42

    Article  CAS  PubMed  Google Scholar 

  • Balamurugan M, Parthasarathi K, Cooper EL, Ranganathan LS (2007) Earthworm paste (Lampito mauritii, Kinberg) alters inflammatory, oxidative, haematological and serum biochemical indices of inflamed rat. Eur Rev Med Pharmacol Sci 11:77–90

    CAS  PubMed  Google Scholar 

  • Balamurugana M, Parthasarathia K, Cooper EL, Ranganathan LS (2009) Anti-inflammatory and anti-pyretic activities of earthworm extract – Lampito mauritii (Kinberg). J Ethnopharmacol 121:330–332

    Article  Google Scholar 

  • Boyd JG, Gordon T (2002) A dose-dependent facilitation and inhibition of peripheral nerve regeneration by brain-derived neurotrophic factor. Eur J Neurosci 15:613–626

    Article  CAS  PubMed  Google Scholar 

  • Bunge RP (1993) Expanding roles for the Schwann cell: ensheathment, myelination, trophism and regeneration. Curr Opin Neurobiol 3:805–809

    Article  CAS  PubMed  Google Scholar 

  • Caroni P, Grandes P (1990) Nerve sprouting in innervated adult skeletal muscle induced by exposure to elevated levels of insulin-like growth factors. J Cell Biol 110:1307–1317

    Article  CAS  PubMed  Google Scholar 

  • Chang YM, Shih YT, Chen YS, Liu CL, Fang WK, Tsai CH, Tsai FJ, Kuo WW, Lai TY, Huang CY (2009a) Schwann cell migration induced by earthworm extract via activation of PAs and MMP2/9 mediated through ERK1/2 and p38. eCAM (Advance Access published online on october 6, 2009; doi: 10.1093/ecam/nep131)

    Google Scholar 

  • Chang YM, Kuo WH, Lai TY, Shih YT, Tsai FJ, Tsai CH, Shu WT, Chen YY, Chen YS, Kuo WW, Huang CY (2009b) RSC96 Schwann cell proliferation and survival induced by earthworm extract through PI3K/Akt signaling mediated by IGF-I. eCAM (Advance Access published online on December 29, 2009; doi:10.1093/ecam/nep216)

    Google Scholar 

  • Chen ZY, Chai YF, Cao L, Lu CL, He C (2001) Glial cell line-derived neurotrophic factor enhances axonal regeneration following sciatic nerve transaction in adult rats. Brain Res 902:272–276

    Article  CAS  PubMed  Google Scholar 

  • Chen H, Takahashi S, Imamura M, Okutani E, Zhang ZG, Chayama K (2007) Earthworm fibrinolytic enzyme: anti-tumor activity on human hepatoma cells in vitro and in vivo. Chin Med J 120:898–904

    CAS  PubMed  Google Scholar 

  • Cheng YS, Cheng WC, Yao CH, Hsieh CL, Lin JG, Lai TY, Lin CC, Tsai CC (2001) Effects of buyang huanwu decoction on peripheral nerve regeneration using silicone rubber chambers. Am J Chin Med 29:423–432

    Article  CAS  PubMed  Google Scholar 

  • Cicco-Bloom E, Black IB (1988) Insulin growth factors regulate the mitotic cycle in cultured rat sympathetic neuroblasts. Proc Natl Acad Sci U S A 85:4066–4070

    Article  Google Scholar 

  • Cikutovic MA, Fitzpatrick LC, Goven AJ, Venables BJ, Giggleman MA, Cooper EL (1999) Wound healing in earthworms Lumbricus terrestris: a cellular-based biomarker for assessing sublethal chemical toxicity. Bull Environ Contam Toxicol 62:508–514

    Article  CAS  PubMed  Google Scholar 

  • Cooper EL (2005a) Bioprospecting: a CAM frontier. eCAM 2:1–3

    PubMed  Google Scholar 

  • Cooper EL (2005b) CAM, eCAM, bioprospecting: the 21st century pyramid. eCAM 2:125–127

    PubMed  Google Scholar 

  • Cooper E (2009) New enzyme complex isolated from earthworms is potent fibrinolytic; lumbrokinase has anti-platelet, anti-thrombatic activity. Integrative Medicine Blog pp 2–5, http://acam.typepad.com/blog/2009/04/new-enzyme-complex-isolated-from-earthworms-is-potent-fibrinolytic-.html, April 10, 2009

  • Cooper EL, Roch P (1992) The capacities of earthworms to heal wounds and to destroy allografts are modified by polychlorinated biphenyls (PCBs). J Invert Pathol 60:59–63

    Article  CAS  Google Scholar 

  • Cooper EL, Hrzenjak TM, Grdisa M (2004) Alternative sources of fibrinolytic, anticoagulative, antimicrobial and anticancer molecules. Int J Immunopathol Pharmacol 17:237–244

    CAS  PubMed  Google Scholar 

  • Eisenhauer N, Straube D, Johnson EA, Parkinson D, Scheu S (2009) Exotic ecosystem engineers change the emergence of plants from the seed bank of a deciduous forest. Ecosystems 12:1008–1016

    Article  CAS  Google Scholar 

  • Fitzpatrick LC, Goven AJ, Venables BJ, Cooper EL (1990) Earthworm immunoassays for evaluating biological effects of exposure to hazardous materials. In: Sandhu SS, Lower WR, de Serres FJ, Suk WA, Tice RR (eds) In situ evaluation of biological hazards of environmental pollutants. Plenum, New York, pp 119–129

    Google Scholar 

  • Gates GE (1974) Contributions to North American earthworms (Annelida). On American earthworm genera. I. Eisenoides (Lumbricidae). Bull Tall Timbers Res Stn 13:1–17

    Google Scholar 

  • Gong JG, McBride D, Bramley TA, Webb R (1993) Effects of recombinant bovine somatotrophin, insulin-like growth factor-I and insulin on the proliferation of bovine granulosa cells in vitro. J Endocrinol 139:67–75

    Article  CAS  PubMed  Google Scholar 

  • Grdisa M, Popovic M, Hrzenjak T (2001) Glycolipoprotein extract (G-90) from earthworm Eisenia foetida exerts some antioxidative activity. Comp Biochem Physiol A Mol Integr Physiol 128:821–825

    Article  CAS  PubMed  Google Scholar 

  • Grdisa M, Popovie M, Hrzenjak T (2004) Stimulation of growth factor synthesis in skin wounds using tissue extract (G-90) from the earthworm Eisenia foetida. Cell Biochem Funct 22:373–378

    Article  CAS  PubMed  Google Scholar 

  • Hasegawa K, Kobayashi H (1991) Immunohistochemical localization of endothelin-1 in the nervous system of the earthworm Eisenia foetida. Gen Comp Endocrinol 81:433–441

    Article  CAS  PubMed  Google Scholar 

  • Hendrix PF, Callaham MC, Drake J, Huang CY, James SW, Snyder BA, Zhang WX (2008) Pandora’s box contained bait: the global problem of introduced earthworms. Annu Rev Ecol Evol Syst 39:593–613

    Article  Google Scholar 

  • Hrzenjak T, Hrzenjak M, Kasuba V, Efenberger-Marinculic P, Levanat S (1992) A new source of biologically active compounds – earthworm tissue (Eisenia foetida, Lumbricus rubelus). Comparative biochemistry & physiology. Comp Physiol 102:441–447

    CAS  Google Scholar 

  • Hrzenjak M, Kobrehel D, Levanat S, Jurin M, Hrzenjak T (1993) Mitogenicity of the earthworm’s (Eisenia foetida) insulin-like proteins. Comp Biochem Physiol B 104:723–729

    Article  CAS  PubMed  Google Scholar 

  • Hrzenjak T, Popovic M, Bozic T, Grdisa M, Kobrehel D, Tiska-Rudman L (1998) Fibrinolytic and anticoagulative activities from the earthworm Eisenia foetida. Comp Biochem Physiol B Biochem Mol Biol 119:825–832

    Article  CAS  PubMed  Google Scholar 

  • Hu W, Fu T (1997) Isolation and properties of a novel fibrinolytic enzyme from an earthworm. Zhong Yao Cai 20:78–81

    CAS  PubMed  Google Scholar 

  • Huang C, Jacobson K, Schaller MD (2004) MAP kinases and cell migration. J Cell Sci 117:4619–628

    Article  CAS  PubMed  Google Scholar 

  • Jessen KR, Mirsky R (2008) Negative regulation of myelination: relevance for development, injury, and demyelinating disease. Glia 56:1552–1565

    Article  PubMed  Google Scholar 

  • Ji H, Wang L, Bi H, Sun L, Cai B, Wang Y (2008) Mechanisms of lumbrokinase in protection of cerebral ischemia. Eur J Pharmacol 590:281–289

    Article  CAS  PubMed  Google Scholar 

  • Jin L, Jin H, Zhang G, Xu G (2000) Changes in coagulation and tissue plasminogen activator after the treatment of cerebral infarction with lumbrokinase. Clin Hemorheol Microcirc 23:213–218

    CAS  PubMed  Google Scholar 

  • Joo SS, Won TJ, Kim JS, Yoo YM, Tak ES, Park SY, Park HY, Hwang KW, Park SC, Leedo I (2009) Inhibition of coagulation activation and inflammation by a novel factor Xa inhibitor synthesized from the earthworm Eisenia andrei. Biol Pharm Bull 32:253–258

    Article  CAS  PubMed  Google Scholar 

  • Kimura H, Kawatani M, Ito E, Ishikawa K (2004) PACAP facilitate the nerve regeneration factors in the facial nerve injury. Regul Pept 123:135–138

    Article  CAS  PubMed  Google Scholar 

  • Lee SH, Kim EY, Hyun S, Kim JG (2009) Metal availability in heavy metal-contaminated open burning and open detonation soil: assessment using soil enzymes, earthworms, and chemical extractions. J Hazard Mater 170:382–388

    Article  CAS  PubMed  Google Scholar 

  • Meintanis S, Thomaidou D, Jessen KR, Mirsky R, Matsas R (2001) The neuron-glia signal beta-neuregulin promotes Schwann cell motility via the MAPK pathway. Glia 34:39–51

    Article  CAS  PubMed  Google Scholar 

  • Pearson G, Robinson F, Beers GT, Xu BE, Karandikar M, Berman K (2001) Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev 22:153–183

    Article  CAS  PubMed  Google Scholar 

  • Popovic M, Hrzenjak T, Grdisa M, Vukovic S (1998) Adhesins of immunoglobulin-like superfamily from earthworm Eisenia foetida. Gen Pharmacol 30:795–800

    Article  CAS  PubMed  Google Scholar 

  • Popovic M, Hrcenjak TM, Babic T, Kos J, Grdisa M (2001) Effect of earthworm (G-90) extract on formation and lysis of clots originated from venous blood of dogs with cardiopathies and with malignant tumors. Pathol Oncol Res 7:197–202

    Article  CAS  PubMed  Google Scholar 

  • Popovic M, Grdisa M, Hrzenjak TM (2005) Glycolipoprotein G-90 obtained from the earthworm Eisenia foetida exerts antibacterial activity. Veterinarski Arhiv 75:119–128

    Google Scholar 

  • Prakash M, Gunasekaran G, Elumalai K (2008) Effect of earthworm powder on antioxidant enzymes in alcohol induced hepatotoxic rats. Eur Rev Med Pharmacol Sci 12:237–243

    CAS  PubMed  Google Scholar 

  • Reddy KB, Nabha SM, Atanaskova N (2003) Role of MAP kinase in tumor progression and invasion. Cancer Metastasis Rev 22:395–403

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez JB, Venables J, Fitzpatrick LC, Goven AJ, Cooper EL (1989) Suppression of secretory rosette formation by PCBs in Lumbricus terrestris: an earthworm immunoassay for humoral immunotoxicity of xenobiotics. Environ Toxicol Chem 8:1201–1207

    Google Scholar 

  • Schumacher M, Jung-Testas I, Robel P, Baulieu EE (1993) Insulin-like growth factor I: a mitogen for rat Schwann cells in the presence of elevated levels of cyclic AMP. Glia 8:232–240

    Article  CAS  PubMed  Google Scholar 

  • Somogyi I, Boros A, Engelmann P, Varhalmi E, Nemeth J, Lubics A, Tamas A, Kiss P, Reglodi D, Pollak E, Molnara L (2009) Pituitary adenylate cyclase-activating polypeptide-like compounds could modulate the activity of coelomocytes in the earthworm. Ann NY Acad Sci 1163:521–523

    Article  CAS  PubMed  Google Scholar 

  • Sullivan KA, Kim B, Feldman EL (2008) Insulin-like growth factors in the peripheral nervous system. Endocrinology 149:5963–5971

    Article  CAS  PubMed  Google Scholar 

  • Sun JH, Gao YM, Yang L, Wang X, Bao LH, Liu WJ, Yew D (2007) Effects of buyang huanwu decoction on neurite outgrowth and differentiation of neuroepithelial stem cells. Chin J Physiol 50:151–156

    PubMed  Google Scholar 

  • Svaren J, Meijer D (2008) The molecular machinery of myelin gene transcription in Schwann cells. Glia 56:1541–1551

    Article  PubMed  Google Scholar 

  • Tang Y, Liang D, Jiang T, Zhang J, Gui L, Chang W (2002) Crystal structure of earthworm fibrinolytic enzyme component a: revealing the structural determinants of its dual fibrinolytic activity. J Mol Biol 321:57–68

    Article  CAS  PubMed  Google Scholar 

  • Varhalmi E, Somogyi I, Kiszler G, Nemeth J, Reglodi D, Lubics A, Kiss P, Tamas A, Pollak E, Molnar L (2008) Expression of PACAP-like compounds during the caudal regeneration of the earthworm Eisenia fetida. J Mol Neurosci 36:166–174

    Article  CAS  PubMed  Google Scholar 

  • Vaudry D, Gonzalez BJ, Basille M, Yon L, Fournier A, Vaudry H (2000) Pituitary adenylate cyclase-activating polypeptide and its receptors: from structure to functions. Pharmacol Rev 52:329–364

    Google Scholar 

  • Wang L, Jiang DM (2009) Neuroprotective effect of Buyang Huanwu decoction on spinal ischemia/reperfusion injury in rats. J Ethnopharmacol 124:219–223

    Article  PubMed  Google Scholar 

  • Wang XQ, Chen L, Pan R, Zhao J, Liu Y, He RQ (2008) An earthworm protease cleaving serum fibronectin and decreasing HBeAg in HepG2.2.15 cells. BMC Biochem 9:30

    Article  CAS  PubMed  Google Scholar 

  • Wei SY, Yin XF, Kou YH, Jiang BG (2009) Lumbricus extract promotes the regeneration of injured peripheral nerve in rats. J Ethnopharmacol 123:51–54

    Article  PubMed  Google Scholar 

  • Witowsky J, Abell A, Johnson NL, Johnson GL, Cuevas BD (2003) MEKK1 is required for inducible urokinase-type plasminogen activator expression. J Biol Chem 278:5941–5946

    Article  CAS  PubMed  Google Scholar 

  • Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, Lin Q, Lin R (2009) Protective effects of Chinese traditional medicine Buyang Huanwu Decoction on myocardial injury. Evid Based Complement Alternat Med. Feb 8. (Epub ahead of print) doi:10.1093/ecam/nep013

    Google Scholar 

  • Zackenfels K, Oppenheim RW, Rohrer H (1995) Evidence for an important role of IGF-I and IGF-II for the early development of chick sympathetic neurons. Neuron 14:731–741

    Article  CAS  PubMed  Google Scholar 

  • Zhang ZX, Wang FF (1992) Effects of crude extract of earthworm on promoting blood circulation to removing stasis. Zhongguo Zhong Xi Yi Jie He Za Zhi 12:741–743

    CAS  PubMed  Google Scholar 

  • Zhao XY, Liu ZM, Jing TY, Wu JX, Zhao ZY (2006) A component of earthworm fibrinolytic enzyme having higher thrombolytic activity than total components in vivo Yao Hsueh Hsueh Pao. Acta Pharmaceutica Sinica 41:1068–1073

    CAS  PubMed  Google Scholar 

  • Zhao J, Pan R, He J, Liu Y, Li DF, He RQ (2007) Eisenia fetida Protease-III-1 functions in both fibrinolysis and fibrogenesis. J Biomed Biotechnol 97:654

    Google Scholar 

Download references

Acknowledgment

This study is supported in part by Taiwan Department of Health Clinical Trial and Research Center of Excellence (DOH99-TD-B-111-004).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chih-Yang Huang .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Berlin Heidelberg

About this chapter

Cite this chapter

Chang, YM., Chi, WY., Cooper, E.L., Kuo, WW., Huang, CY. (2011). Earthworms: A Potent Herbal Target for TCM (CAM) Research. In: Karaca, A. (eds) Biology of Earthworms. Soil Biology, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14636-7_15

Download citation

Publish with us

Policies and ethics