An Overview of HPTLC: A Modern Analytical Technique with Excellent Potential for Automation, Optimization, Hyphenation, and Multidimensional Applications

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Abstract

High performance thin layer chromatography (HPTLC) is a sophisticated instrumental technique based on the full capabilities of thin layer chromatography. The advantages of automation, scanning, full optimization, selective detection principle, minimum sample preparation, hyphenation, etc. enable it to be a powerful analytical tool for chromatographic information of complex mixtures of inorganic, organic, and biomolecules. The chapter highlights related issues such as journey of thin-layer chromatography, basic principle, protocol, separation, resolution, validation process, recent developments, and modifications on TLC leading to the HPTLC, optimization, process control, automation, and hyphenation. It explains that HPTLC has strong potentials as a surrogate chromatographic model for estimating partitioning properties in support of combinatorial chemistry, environmental fate, and health effect studies.