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Structures and Organelles in Pathogenic Protists pp 149–173Cite as

Organelles and Trafficking in Entamoeba histolytica

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Part of the book series: Microbiology Monographs ((MICROMONO,volume 17))

Abstract

Publication of the Entamoeba histolytica genome has provided strong support for cell biology studies on this lower eukaryote, in which clear cellular compartments involving vesicular trafficking have not yet been well established. Protein trafficking through the endoplasmic reticulum and the Golgi apparatus is an important issue linked to pathogenesis due to the important number of factors secreted by this parasite to accomplish cell killing, tissue invasion, and the onset of inflammation in humans. The advancements in the cellular and molecular tools available have allowed previously unidentifiable organelles to be identified based on gene homologies and video microscopy. The aim of this chapter is to provide a review of the current literature as it pertains to Entamoeba histolytica organelles, protein transport, and nutrient uptake, specifically by examining the Golgi, ER, mitosome, phagocytosis, and endocytosis.

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References

  • Aalto MK, Ronne H, Keranen S (1993) Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J 12:4095–4104

    PubMed  CAS  Google Scholar 

  • Aguilera P, Barry T, Tovar J (2008) Entamoeba histolytica microsomes: organelles in search of a function. Exp Parasitol 118:10–16

    Article  PubMed  CAS  Google Scholar 

  • Ali V, Shigeta Y, Tokumoto U, Takahashi Y, Nozaki T (2004) An intestinal parasitic protist, Entamoeba histolytica, possesses a non-redundant nitrogen fixation-like system for iron-sulphur cluster assembly under anaerobic conditions. J Biol Chem 279:16863–16874

    Article  PubMed  CAS  Google Scholar 

  • Ali IK, Clark CG, Petri WA Jr (2008) Molecular epidemiology of amebiasis. Infect Genet Evol 8:698–707

    Article  PubMed  CAS  Google Scholar 

  • Almeida IC, Camargo MM, Procópio DO, Silva LS, Mehlert A, Travassos LR, Gazzinelli RT, Ferguson MA (2000) Highly purified glycosylphosphatidylinositols from Trypansoma cruzi are potent proinflammatory agents. EMBO J 19:1476–1485

    Article  PubMed  CAS  Google Scholar 

  • Andrä J, Herbst R, Leippe M (2003) Amoebapores, archaic effector peptides of protozoan origin, are discharged into phagosomes and kill bacteria by permeabilizing their membranes. Dev Comp Immunol 27:291–304

    Article  PubMed  Google Scholar 

  • Appenzeller-Herzog C, Roch A, Nufer O, Hauri H (2004) pH-induced conversion of the transport Lectin ERGIC-53 triggers glycoprotein release. J Biol Chem 279:12943–12950

    Article  PubMed  CAS  Google Scholar 

  • Arighi CN, Hartnell LM, Aguilar RC, Haft CR, Bonifacino JS (2004) Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol 165:123–133

    Article  PubMed  CAS  Google Scholar 

  • Bakatselou C, Kidgell C, Clark CG (2000) A mitochondrial-type hsp70 gene of E. histolytica. Mol Biochem Parasitol 110:177–182

    Article  PubMed  CAS  Google Scholar 

  • Banerjee S, Vishwanath P, Cui J, Kelleher DJ, Gilmore R, Robbins PW, Samuelson J (2007) The evolution of N-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation. Proc Natl Acad Sci USA 104:11676–11681

    Article  PubMed  CAS  Google Scholar 

  • Banerjee S, Cui J, Robbins PW, Samuelson J (2008) Use of Giardia, which appears to have a single nucleotide-sugar transporter for UDP-GlcNAc, to identify the UDP-Glc transporter of Entamoeba. Mol Biochem Parasitol 159:44–53

    Article  PubMed  CAS  Google Scholar 

  • Banfield DK, Lewis MJ, Pelham HR (1995) A SNARE-like protein required for traffic through the Golgi complex. Nature 375:806–809

    Article  PubMed  CAS  Google Scholar 

  • Bassoe CF, Bjerknes R (1985) Phagocytosis by human leukocytes, phagosomal pH and degradation of seven species of bacteria measured by flow cytometry. J Med Microbiol 19:115–125

    Article  PubMed  CAS  Google Scholar 

  • Batista EJ, de Menezes Feitosa LF, de Souza W (2000) The endocytic pathway in Entamoeba histolytica. Parasitol Res 86:881–890

    Article  PubMed  CAS  Google Scholar 

  • Bennett MK, Calakos N, Scheller RH (1992) Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones. Science 257:255–259

    Article  PubMed  CAS  Google Scholar 

  • Beyenback KW, Wieczorek H (2006) The V-type H+ATPase: molecular structure and function, physiological roles and regulation. J Exp Biol 209:577–589

    Article  CAS  Google Scholar 

  • Bock JB, Matern HT, Peden AA, Scheller RH (2001) A genomic perspective on membrane compartment organization. Nature 409:839–841

    Article  PubMed  CAS  Google Scholar 

  • Boettner DR, Huston CD, Sullivan JA, Petri WA Jr (2005) Entamoeba histolytica and Entamoeba dispar utilize externalized phosphatidylserine for recognition and phagocytosis of erythrocytes. Infect Immun 73:3422–3430

    Article  PubMed  CAS  Google Scholar 

  • Boettner DR, Huston CD, Linford AS, Buss SN, Houpt E, Sherman NE, Petri WA Jr (2008) Entamoeba histolytica phagocytosis of human erythrocytes involves PATMK, a member of the transmembrane kinase family. PLoS Pathog 4:0122–0133

    Article  CAS  Google Scholar 

  • Bonificino JS, Glick BS (2004) The mechanisms of vesicle budding and fusion. Cell 116:153–166

    Article  Google Scholar 

  • Bos HJ, Van de Griend RJ (1977) Virulence and toxicity of axenic Entamoeba histolytic. Nature 265:341–343

    Article  PubMed  CAS  Google Scholar 

  • Botelho RJ, Scott CC, Grinstein S (2004) Phosphoinositide involvement in phagocytosis and phagosome maturation. Curr Top Microbiol Immunol 282:1–30

    Article  PubMed  CAS  Google Scholar 

  • Brandolin G, Le Saux A, Trezeguet V, Lauquin GJ, Vignais PV (1993) Chemical immunological, enzymatic, and genetic approaches to studying the arrangement of the peptide chain of the ADP/ATP carrier in the mitochondrial membrane. J Bioenerg Biomembr 25:459–472

    Article  PubMed  CAS  Google Scholar 

  • Bravo-Torres JC, Villagómez-Castro JC, Calvo-Méndez C, Flores-Carreón A, López-Romero E (2004) Purification and biochemical characterization of a membrane-bound α-glucosidase from the parasite Entamoeba histolytica. Int J Parasitol 34:455–462

    Article  PubMed  CAS  Google Scholar 

  • Bredeston LM, Caffaro CE, Samuelson J, Hirschberg CB (2005) Golgi and endoplasmic reticulum functions take place in different subcellular compartments of Entamoeba histolytica. J Biol Chem 280:32168–32176

    Article  PubMed  CAS  Google Scholar 

  • Bruchhaus I, Jacobs T, Leippe M, Tannich E (1996) Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. Mol Microbiol 22:255–263

    Article  PubMed  CAS  Google Scholar 

  • Bruchhaus I, Loftus BJ, Hall N, Tannich E (2003) The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation. Eukaryot Cell 2:501–509

    Article  PubMed  CAS  Google Scholar 

  • Bruhn H, Riekens B, Berninghausen O, Leippe M (2003) Amoebapores and NK-lysin, members of a class of structurally distinct antimicrobial and cytolytic peptides from protozoa and mammals: a comparative functional analysis. Biochem J 375:737–744

    Article  PubMed  CAS  Google Scholar 

  • Bucci C, Lütcke A, Steele-Mortimer O, Olkkonen VM, Dupree P, Chiariello M, Bruni CB, Simons K, Zerial M (1995) Co-operative regulation of endocytosis by three Rab5 isoforms. FEBS Lett 366:65–71

    Article  PubMed  CAS  Google Scholar 

  • Burdett ID (2002) Effects of Brefeldin A on disassembly of the Golgi body in MDCK cells subjected to a Ca2+ shift at low temperature. Eur J Cell Biol 81:525–528

    Article  PubMed  CAS  Google Scholar 

  • Caffaro CE, Hirschberg CB (2006) Nucleotide sugar transporters of the Golgi apparatus: from basic science to diseases. Acc Chem Res 39:805–812

    Article  PubMed  CAS  Google Scholar 

  • Chan KW, Slotboom DJ, Cox S, Embley TM, Fabre O, van der Giezen M, Harding M, Horner DS, Kunji ERS, Leon-Avila G, Tovar J (2005) A novel ADP/ATP transporter in the mitosome of the microaerophilic human parasite Entamoeba histolytica. Curr Biol 15:737–742

    Article  PubMed  CAS  Google Scholar 

  • Chavrier P, Goud B (1999) The role of ARF and Rab GTPases in membrane transport. Curr Opin Cell Biol 11:466–475

    Article  PubMed  CAS  Google Scholar 

  • Clark CG, Roger AJ (1995) Direct evidence for secondary loss of mitochondria in Entamoeba histolytica. PNAS 92:6518–6521

    Article  PubMed  CAS  Google Scholar 

  • Clark CG, Alsmark UC, Tazreiter M, Saito-Nakano Y, Ali V, Marion S, Weber C, Mukherjee C, Bruchhaus I, Tannich E, Leippe M, Sicheritz-Ponten T, Foster PG, Samuelson J, Noël CJ, Hirt RP, Embley TM, Gilchrist CA, Mann BJ, Singh U, Ackers JP, Bhattacharya S, Bhattacharya A, Lohia A, Guillén N, Duchêne M, Nozaki T, Hall N (2007) Structure and content of the Entamoeba histolytica genome. Adv Parasitol 65:51–190

    Article  PubMed  CAS  Google Scholar 

  • Cruz-Castañeda A, Hernández-Sánchez J, Olivares-Trejo JJ (2009) Cloning and identification of a gene coding for a 26-kDa hemoglobin-binding protein from Entamoeba histolytica. Biochimie 91:383–389

    Article  PubMed  CAS  Google Scholar 

  • Dacks JB, Doolittle WF (2004) Molecular and phylogenetic characterization of syntaxin genes from parasitic protozoa. Mol Biochem Parasitol 136:123–136

    Article  PubMed  CAS  Google Scholar 

  • Dacks JB, Davis LA, Sjögren AM, Andersson JO, Roger AJ, Doolittle WF (2003) Evidence for Golgi bodies in proposed ‘Golgi-lacking’ lineages. Proc Biol Sci 270:S168–S171

    Article  PubMed  Google Scholar 

  • Das SR, Ghoshal S (1976) Restoration of virulence to rat of axenically grown Entamoeba histolytica by cholesterol and hamster liver passage. Ann Trop Med Parasitol 70:439–443

    PubMed  CAS  Google Scholar 

  • Das S, Lohia A (2002) Delinking of S phase and cytokinesis in the protozoan parasite Entamoeba histolytica. Cell Microbiol 4:55–60

    Article  PubMed  CAS  Google Scholar 

  • Dautry-Varsat A (1986) Receptor-mediated endocytosis: the intracellular journey of transferrin and its receptor. Biochimie 68:375–381

    Article  PubMed  CAS  Google Scholar 

  • de la Vega H, Specht CA, Semino CE, Robbins PW, Eichinger D, Caplivski D, Ghosh S, Samuelson J (1997) Cloning and expression of chitinases of Entamoebae. Mol Biochem Parasitol 85:139–147

    Article  PubMed  Google Scholar 

  • Descoteaux S, Yu Y, Samuelson J (1994) Cloning of Entamoeba genes encoding proteolipids of putative vacuolar proton-translocating ATPases. Infect Immun 62:3572–3575

    PubMed  CAS  Google Scholar 

  • Diamond LS, Phillips BP, Bartgis IL (1974) A comparison of the virulence of nine strains of axenically cultivated Entamoeba histolytica in hamster liver. Archivos de Investigacion Medica 5:423–426

    PubMed  Google Scholar 

  • Field H, Farjah M, Pal A, Gull K, Field MC (1998) Complexity of trypanosomatid endocytosis pathways revealed by Rab4 and Rab5 isoforms in Trypanosoma brucei. J Biol Chem 273:32102–32110

    Article  PubMed  CAS  Google Scholar 

  • Fok AK, Lee Y, Allen RD (1982) The correlation of digestive vacuole pH and size with the digestive cycle in Paramecium caudatum. J Protozool 29:409–414

    Google Scholar 

  • Fratti RA, Backer JM, Gruenberg J, Corvera S, Deretic V (2001) Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest. J Cell Biol 154:631–644

    Article  PubMed  CAS  Google Scholar 

  • Geisow MJ, D’Arcy Hart P, Young MR (1981) Temporal changes of lysosome and phagosome pH during phagolysosome formation in macrohages: studies by fluorescence spectroscopy. J Cell Biol 89:645–652

    Article  PubMed  CAS  Google Scholar 

  • Ghosh SK, Samuelson J (1997) Involvement of p21racA, phosphoinositide 3-kinase, and vacuolar ATPase in phagocytosis of bacteria and erythrocytes by Entamoeba histolytica: suggestive evidence for coincidental evolution of amebic invasiveness. Infect Immun 65:4243–4249

    PubMed  CAS  Google Scholar 

  • Ghosh SK, Field J, Frisardi M, Rosenthal B, Mai Z, Rogers R, Samuelson J (1999) Chitinase secretion by encysting Entamoeba invadens and Transfected Entamoeba histolytica Trophozoites: localization of secretory vesicles, endoplasmic reticulum, and Golgi apparatus. Infect Immun 67:3073–3081

    PubMed  CAS  Google Scholar 

  • Ghosh S, Field J, Rogers R, Hickman M, Samuelson J (2000) The Entamoeba histolytica mitochondrion-derived organelle (crypton) contains double-stranded DNA and appears to be bound by a double membrane. Infect Immun 68:4319–4322

    Article  PubMed  CAS  Google Scholar 

  • Gillooly DJ, Simonsen A, Stenmark H (2001) Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins. Biochem J 355:249–258

    Article  PubMed  CAS  Google Scholar 

  • Girard-Misguich F, Sachse M, Santi-Rocca J, Guillén N (2008) The endoplasmic reticulum chaperone calreticulin is recruited to the uropod during capping of surface receptors in Entamoeba histolytica. Mol Biochem Parasitol 157:236–240

    Article  PubMed  CAS  Google Scholar 

  • Goldberg AV, Molik S, Tsaousis AD, Neumann K, Kuhnke G, Delbac F, Vivares CP, Hirt RP, Lill R, Embley TM (2008) Localization and functionality of microsporidian iron-sulphur cluster assembly proteins. Nature 452:624–628

    Article  PubMed  CAS  Google Scholar 

  • Groves E, Dart AE, Covarelli V, Caron E (2008) Molecular mechanisms of phagocytic uptake in mammalian cells. Cell Mol Life Sci 65:1957–1976

    Article  PubMed  CAS  Google Scholar 

  • Hackstein JHP, Tjaden J, Huynen M (2006) Mitochondria. hydrogenosomes and mitosomes: products of evolutionary tinkering. Curr Genet 50:225–245

    Article  PubMed  CAS  Google Scholar 

  • Hartman DJ, Hoogenraad NJ, Condron R, Hoj PB (1992) Identification of a mammalian 10-kDa heat shock protein, a mitochondrial chaperonin 10 homologue essential for assisted folding of trimeric ornithine transcarbamoylase in vitro. PNAS 89:3394–3398

    Article  PubMed  CAS  Google Scholar 

  • Hauri H, Kappeler F, Andersson H, Appenzeller C (2000) ERGIC-53 and traffic in the secretory pathway. J Cell Sci 113:587–596

    PubMed  CAS  Google Scholar 

  • Helenius A, Aebi M (2004) Roles of N-linked glycans in the Endoplasmic Reticulum. Annu Rev Biochem 73:1019–1049

    Article  PubMed  CAS  Google Scholar 

  • Hirschberg CB, Robbins PW, Abeijon C (1998) Transporters of nucleotide sugars, ATP, and nucleotide sulfate in the endoplasmic reticulum and Golgi apparatus. Annu Rev Biochem 67:49–69

    Article  PubMed  CAS  Google Scholar 

  • Hume AN, Collinson LM, Rapak A, Gomes AQ, Hopkins CR, Seabra MC (2001) Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J Cell Biol 152:795–808

    Article  PubMed  CAS  Google Scholar 

  • Huston CD, Houpt ER, Mann BJ, Hahn CS, Petri WA Jr (2000) Caspase 3-dependent killing of host cells by the parasite Entamoeba histolytica. Cell Microbiol 2:617–625

    Article  PubMed  CAS  Google Scholar 

  • Huston CD, Boettner DR, Miller-Sims V, Petre WA Jr (2003) Apoptotic killing and phagocytosis of host cells by the parasite Entamoeba histolytica. Infect Immun 71:964–972

    Article  PubMed  CAS  Google Scholar 

  • Jensen MS, Bainton DF (1973) Temporal changes in pH within the phagocytic vacuole of the polymorphonuclear neutrophilic leukocyte. J Cell Biol 56:379–388

    Article  PubMed  CAS  Google Scholar 

  • Kane P (2006) The where, when, and how of organelle acidification by the yeast vacuolar H+-ATPase. Microbiol Mol Biol Rev 70:177–191

    Article  PubMed  CAS  Google Scholar 

  • Katiyar SK, Edlind T (1996) Entamoeba histolytica encodes a highly divergent β-tubulin. J Eukaryot Microbiol 43:31–34

    Article  PubMed  CAS  Google Scholar 

  • Labruyère E, Zimmer C, Galy V, Olivo-Marin JC, Guillén N (2003) EhPAK, a member of the p21-activated kinase family, is involved in the control of Entamoeba histolytica migration and phagocytosis. J Cell Sci 116:61–71

    Article  PubMed  CAS  Google Scholar 

  • Lal K, Field MC, Carlton JM, Warwicker J, Hirt RP (2005) Identification of a very large Rab GTPase family in the parasitic protozoan Trichomonas vagnalis. Mol Biochem Parasitol 143:226–235

    Article  PubMed  CAS  Google Scholar 

  • Lang F, Gulbins E, Szabo I, Lepple-Wienhues A, Huber SM, Duranton C, Lang KS, Lang PA, Wieder T (2004) Cell volume and the regulation of apoptotic cell death. J Mol Recognit 17:473–480

    Article  PubMed  CAS  Google Scholar 

  • Leippe M (1997) Amoebapores. Parasitol Today 13:178–183

    Article  PubMed  CAS  Google Scholar 

  • Leippe M (1999) Antimicrobial and cytolytic polypeptides of amoeboid protozoa–effector molecules of primitive phagocytes. Dev Comp Immunol 23:267–279

    Article  PubMed  CAS  Google Scholar 

  • Leippe M, Andra J, Nickel R, Rannich E, Muller-Eberhard HJ (1994) Amoebapores, a family of membranolytic peptides from cytoplasmic granules of Entamoeba histolytica: isolation, primary structure, and pore formation in bacterial cytoplasmic membranes. Mol Microbiol 15:895–904

    Article  Google Scholar 

  • Lejeune A, Gicquaud C (1987) Evidence for two mechanisms of human erythrocyte endocytosis by Entamoeba histolytica-like amoebae (Laredo strain). Biol Cell 59:239–245

    Article  PubMed  CAS  Google Scholar 

  • Lejeune A, Gicquaud C (1992) Target cell deformability determines the type of phagocytic mechanism used by Entamoeba histolytica-like, Laredo strain. Biol Cell 74:211–216

    Article  PubMed  CAS  Google Scholar 

  • León-Avila G, Tovar J (2004) Mitosomes of Entamoeba histolytica abundant mitochondrial-related remnant organelles that lack a detectable organellar genome. Microbiology 150:1245–1250

    Article  PubMed  CAS  Google Scholar 

  • León-Sicairos N, Reyes-López M, Canizalez-Román A, Bermúdez-Cruz RM, Serrano-Luna J, Arroyo R, de la Garza M (2005) Human hololactoferrin: endocytosis and use as an iron source by the parasite Entamoeba histolytica. Microbiology 151:3859–3871

    Article  PubMed  CAS  Google Scholar 

  • Libros-Ziv P, Villalobo E, Mirelman D (2005) E. histolytica: identification and characterization of an N-ethylmaleimide sensitive fusion protein homologue. Exp Parasitol 110:276–279

    Article  PubMed  CAS  Google Scholar 

  • Lill R, Mühlenhoff U (2006) Iron-sulphur protein biogenesis in eukaryotes: components and mechanisms. Annu Rev Cell Dev Biol 22:457–486

    Article  PubMed  CAS  Google Scholar 

  • Lill R, Mühlenhoff U (2008) Maturation of iron-sulfur proteins in eukaryotes: mechanisms, connected processes, and diseases. Annu Rev Biochem 77:669–700

    Article  PubMed  CAS  Google Scholar 

  • Loftus B, Anderson I, Davies R, Alsmark UC, Samuelson J, Amedeo P, Roncaglia P, Berriman M, Hirt RP, Mann BJ, Nozaki T, Suh B, Pop M, Duchene M, Ackers J, Tannich E, Leippe M, Hofer M, Bruchhaus I, Willhoeft U, Bhattacharya A, Chillingworth T, Churcher C, Hance Z, Harris B, Harris D, Jagels K, Moule S, Mungall K, Ormond D, Squares R, Whitehead S, Quail MA, Rabbinowitsch E, Norbertczak H, Price C, Wang Z, Guillén N, Gilchrist C, Stroup SE, Bhattacharya S, Lohia A, Foster PG, Sicheritz-Ponten T, Weber C, Singh U, Mukherjee C, El-Sayed NM, Petri WA Jr, Clark CG, Embley TM, Barrell B, Fraser CM, Hall N (2005) The genome of the protist parasite Entamoeba histolytica. Nature 433:865–868

    Article  PubMed  CAS  Google Scholar 

  • Lohia A (2003) The cell cycle of Entamoeba histolytica. Mol Cell Biochem 253:217–222

    Article  PubMed  CAS  Google Scholar 

  • Lohia A, Mukherjee C, Majumder S, Dastidar PG (2007) Genome re-duplication and irregular segregation occur during the cell cycle of Entamoeba histolytica. Biosci Rep 27:373–384

    Article  PubMed  CAS  Google Scholar 

  • López-Soto F, González-Robles A, Salazar-Villatoro L, León-Sicairos N, Piña-Vázquez C, Salazar EP, de la Garza M (2009) Entamoeba histolytica uses ferritin as an iron source and internalises this protein by means of clathrin-coated vesicles. Int J Parasitol 39:417–426

    Article  PubMed  CAS  Google Scholar 

  • Lucht E, Evengård B, Skott J, Pehrson P, Nord CE (1998) Entamoeba gingivalis in human immunodeficiency virus type 1-infected patients with periodontal disease. Clin Infect Dis 27:471–473

    Article  PubMed  CAS  Google Scholar 

  • Lushbaugh WB, Kairalla AB, Loadholt CB, Pittman FE (1978) Effect of hamster liver passage on the virulence of axenically cultivated Entamoeba histolytica. Am J Trop Med Hyg 27:248–254

    PubMed  CAS  Google Scholar 

  • Luzio JP, Pryor PR, Bright NA (2007) Lysosomes: fusion and function. Nat Rev Mol Cell Biol 8:622–632

    Article  PubMed  CAS  Google Scholar 

  • Manning-Cela R, Marquez C, Franco E, Talamas-Rahana P, Meza I (2003) BFA-sensitive and insensitive exocytic pathways in Entamoeba histolytica trophozoites: their relationship to pathogenesis. Cell Microbiol 5:921–932

    Article  PubMed  CAS  Google Scholar 

  • Marion S, Wilhelm C, Voigt H, Bacri C, Guillén N (2004) Overexpression of myosin IB in living Entamoeba histolytica enhances cytoplasm viscosity and reduces phagocytosis. J Cell Sci 117:3271–3279

    Article  PubMed  CAS  Google Scholar 

  • Marion S, Laurent C, Guillén N (2005) Signalization and cytoskeleton activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach. Cell Microbiol 7:1504–1518

    Article  PubMed  CAS  Google Scholar 

  • Martin J (1997) Molecular chaperones and mitochondrial protein folding. J Bioenerg Biomembr 29:35–43

    Article  PubMed  CAS  Google Scholar 

  • Matouschek A, Pfanner N, Voos W (2000) Protein unfolding by mitochondria. The Hsp70 import motor. EMBO Rep 1:404–410

    Article  PubMed  CAS  Google Scholar 

  • Mattern CF, Keister DB, Natovitz PC (1982) Virulence of Entamoeba histolytica upon continuous axenic cultivation. Archivos de Investigacion Nedica 13:185–190

    Google Scholar 

  • Mayer A (2001) What drives membrane fusion in eukaryotes? Trends Biochem Sci 26:717–723

    Article  PubMed  CAS  Google Scholar 

  • Mazzuco A, Benchimol M, De Souza W (1997) Endoplasmic reticulum and Golgi-like elements in Entamoeba. Micron 28:241–247

    Article  PubMed  CAS  Google Scholar 

  • McGugan GC Jr, Temesvari LA (2003) Characterization of a Rab11-like GTPase, EhRab11, of Entamoeba histolytica. Mol Biochem Parasitol 129:137–146

    Article  PubMed  CAS  Google Scholar 

  • McNew JA, Parlati F, Fukuda R, Johnston RJ, Paz K, Paument F, Söllner TH, Rothman JE (2000) Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature 407:153–159

    Article  PubMed  CAS  Google Scholar 

  • Meléndez-Hernández MG, Barrios MLL, Orozco E, Luna-Arias JP (2008) The Vacuolar ATPase from Entamoeba histolytica: Molecular cloning of the gene encoding for the B subunit and subcellular localization of the protein. BMC Microbiol 8:235

    Article  PubMed  CAS  Google Scholar 

  • Mellman I, Warren G (2000) The road taken: past and future foundations of membrane traffic. Cell 100:99–112

    Article  PubMed  CAS  Google Scholar 

  • Meza I, Clarke M (2004) Dynamics of endocytic traffic of Entamoeba histolytica revealed by confocal microscopy and flow cytometry. Cell Motil Cytoskeleton 59:215–226

    Article  PubMed  CAS  Google Scholar 

  • Meza I, Talamás-Rohana P, Vargas MA (2006) The cytoskeleton of Entamoeba histolytica: structure, function, and regulation by signaling pathways. Arch Med Res 37:234–243

    Article  PubMed  CAS  Google Scholar 

  • Mitra BN, Yasuda T, Kobayashi S, Saito-Nakano Y, Nozaki T (2005) Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar. Cell Motil Cytoskeleton 62:84–99

    Article  PubMed  CAS  Google Scholar 

  • Mitra BN, Kobayashi S, Saito-Nakano Y, Nozaki T (2006) Entamoeba histolytica: differences in phagosome acidification and degradation between attenuated and virulent strains. Exp Parasitol 114:57–61

    Article  PubMed  CAS  Google Scholar 

  • Mitra BN, Saito-Nakano Y, Nakada-Tsukui K, Sato D, Nozaki T (2007) Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiol 9:2112–2125

    Article  PubMed  CAS  Google Scholar 

  • Moremen KW, Molinari M (2006) N-linked glycan recognition and processing: the molecular basis of endoplasmic reticulum quality control. Curr Opin Struct Biol 16:592–599

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee C, Clark CG, Lohia A (2008) Entamoeba shows reversible variation in ploidy under different growth conditions and between life cycle phases. PLoS Negl Trop Dis 2:e281

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee C, Majumder S, Lohia A (2009) Inter-cellular variation in DNA content of Entamoeba histolytica originates from temporal and spatial uncoupling of cytokinesis from the nuclear cycle. PLoS Negl Trop Dis 3:e409

    Article  PubMed  CAS  Google Scholar 

  • Nakada-Tsukui K, Saito-Nakano Y, Ali V, Nozaki T (2005) A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica. Mol Biol Cell 16:5294–5303

    Article  PubMed  CAS  Google Scholar 

  • Nakada-Tsukui K, Okada H, Mitra BN, Nozaki T (2009) Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica. Cell Microbiol 11:1471–1491

    Article  PubMed  CAS  Google Scholar 

  • Nickel W, Weber T, McNew JA, Parlati F, Söllner TH, Rothman JE (1999) Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs. PNAS 96:12571–12576

    Article  PubMed  CAS  Google Scholar 

  • Nishi T, Forgac M (2002) The vacuolar (H+)-ATPases-natures’ most versatile proton pumps. Nat Rev Mol Cell Biol 3:94–103

    Article  PubMed  CAS  Google Scholar 

  • Nothwehr SF, Bruinsma P, Strawn LA (1999) Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment. Mol Biol Cell 10:875–890

    PubMed  CAS  Google Scholar 

  • Novick P, Zerial M (1997) The diversity of Rab proteins in vesicle transport. Curr Opin Cell Biol 9:496–504

    Article  PubMed  CAS  Google Scholar 

  • Nozaki T, Nakada-Tsukui K (2006) Membrane trafficking as a virulence mechanisms of the protozoan parasite Entamoeba histolytica. Parasitol Res 98:179–183

    Article  PubMed  Google Scholar 

  • Ogden CA, de Cathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM (2001) C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells. J Exp Med 194:781–795

    Article  PubMed  CAS  Google Scholar 

  • Okada M, Huston CD, Mann BJ, Petri WA Jr, Kita K, Nozaki T (2005) Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica. Eukaryot Cell 4:827–831

    Article  PubMed  CAS  Google Scholar 

  • Olivos-García A, Saavedra E, Martínez RD (2009) Molecular nature of virulence in Entamoeba histolytica. Infect Genet Evol. doi:10.1016/j.meegid.2009.04.005

    PubMed  Google Scholar 

  • Olkkonen VM, Stenmark H (1997) Role of Rab GTPases in membrane traffic. Int Rev Cytol 176:1–85

    Article  PubMed  CAS  Google Scholar 

  • Orozco E, Guarneros G, Martinez-Palomo A, Sanchez T (1983) Entamoeba histolytica. Phagocytosis as a virulence factor. J Exp Med 158:1511–1521

    Article  PubMed  CAS  Google Scholar 

  • Padilla-Vaca F, Ankri S, Bracha R, Koole LA, Mirelman D (1999) Down regulation of Entamoeba histolytica virulence by monoxenic cultivation with Escherichia coli O55 is related to a decrease in expression of the light (35-kilodalton) subunit of the Gal/GalNAc lectin. Infect Immun 67:2096–2102

    PubMed  CAS  Google Scholar 

  • Paul P, Kamisaka Y, Marks DL, Pagano RE (1996) Purification and characterization of UDP-glucose:ceramide glucosyltransferase from rat liver Golgi membranes. J Biol Chem 271:2287–2293

    Article  PubMed  CAS  Google Scholar 

  • Petri WA, Haque R, Mann BJ (2002) The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica. Annu Rev Microbiol 56:34–64

    Article  CAS  Google Scholar 

  • Phillips BP (1973) Entamoeba histolytica: concurrent irreversible loss of infectivity-pathogenicity and encystment potential after prolonged maintenance in axenic culture in vitro. Exp Parasitol 34:163–167

    Article  PubMed  CAS  Google Scholar 

  • Picazarri K, Luna-Arias JP, Carrillo E, Orozco E, Rodriguez MA (2005) Entamoeba histolytica: Identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB. Exp Parasitol 110:253–258

    Article  PubMed  CAS  Google Scholar 

  • Pimenta PF, Diamond LS, Mirelman D (2002) Entamoeba histolytica Schaudinn, 1903 and Entamoeba dispar Brumpt, 1925: differences in their cell surfaces and in the bacteria-containing vacuoles. J Eukaryot Microbiol 49:209–219

    Article  PubMed  Google Scholar 

  • Powell RR, Welter BH, Hwu R, Bowersox B, Temesvari LA (2006) FYVE-finger domains, phosphatidylinositol 3-phosphate biosensors associate with phagosomes but not fluid filled endosomes in Entamoeba histolytica. Exp Parasitol 112:221–231

    Article  PubMed  CAS  Google Scholar 

  • Putignani L, Tait A, Smith HV, Horner D, Tovar J, Tetley L, Wastling JM (2004) Characterization of a mitochondrion-like organelle in Cryptosporidium parvum. Parasitology 129:1–18

    Article  PubMed  CAS  Google Scholar 

  • Que X, Reed SL (2000) Cysteine proteinases and the pathogensis of amebiasis. Clin Microbiol Rev 13:196–206

    Article  PubMed  CAS  Google Scholar 

  • Que X, Brinen SL, Perkins P, Herdman S, Hirata K, Torian BE, Rubin H, McKerrow JH, Reed SL (2002) Cysteine proteinases from distinct cellular compartments are recruited to phagocytic vesicles by Entamoeba histolytica. Mol Biochem Parasitol 119:23–32

    Article  PubMed  CAS  Google Scholar 

  • Rabinowitz S, Horstmann H, Gordon S, Griffiths G (1992) Immunocytochemical characterization of the endocytic and phagolysomal compartments in peritoneal macrophages. J Cell Biol 116:95–112

    Article  PubMed  CAS  Google Scholar 

  • Raiborg C, Bremnes B, Mehlum A, Gillooly DJ, D'Arrigo A, Stang E, Stenmark HJ (2001) FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes. J Cell Sci 114:2255–2263

    PubMed  CAS  Google Scholar 

  • Ravdin JI (1988) Amebiasis: human infection by Entamoeba histolytica. Wiley, New York

    Google Scholar 

  • Ray SS, Gangopadhyay SS, Pande G, Samuelson J, Lohia A (1997) Primary structure of Entamoeba histolytica γ-tubulin and localization of amoebic microtubule organizing center. Mol Biochem Parasitol 90:331–336

    Article  PubMed  CAS  Google Scholar 

  • Reddy JV, Seaman MN (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12:3242–3256

    PubMed  CAS  Google Scholar 

  • Reyes-López M, Serrano-Luna JJ, Negrete-Abascal E, León-Sicairos N, Guerrero-Barrera AL, de la Garza M (2001) Entamoeba histolytica: transferrin binding proteins. Exp Parasitol 99:132–140

    Article  PubMed  CAS  Google Scholar 

  • Riordan CE, Ault JG, Langreth SG, Keithly JS (2003) Cryptosporidium parvum Cpn6 targets a relict organelle. Curr Genet 44:138–147

    Article  PubMed  CAS  Google Scholar 

  • Rodriguez MA, Orozco E (1986) Isolation and characterization of phagocytosis- and virulence-deficient mutants of Entamoeba histolytica. J Infect Dis 154:27–32

    Article  PubMed  CAS  Google Scholar 

  • Rodriguez MA, Garcia-Perez RM, Garcia-Rivera G, Lopez-Reyes I, Mendoz L, Ortiz-Navarrete V, Orozco E (2000) An Entamoeba histolytica Rab-like encoding gene and protein: function and cellular location. Mol Biochem Parasitol 108:199–206

    Article  PubMed  CAS  Google Scholar 

  • Roy D, Lohia A (2004) Sequence divergence of Entamoeba histolytica tubulin is responsible for its altered tertiary structure. Biochem Biophys Res Commun 319:1010–1016

    Article  PubMed  CAS  Google Scholar 

  • Rupper AC, Rodriguez-Paris JM, Grove BD, Cardellit JA (2001) p110-related PI 3-kinases regulate phagosome-phagosome fusion and phagosomal pH through a PKB/Akt dependent pathway in Dictyostelium. J Cell Sci 114:1283–1295

    PubMed  CAS  Google Scholar 

  • Saito-Nakano Y, Nakazawa M, Shigeta Y, Takeuchi T, Nozaki T (2001) Identification and characterization of genes encoding novel Rab proteins from Entamoeba histolytica. Mol Biochem Parasitol 116:219–222

    Article  PubMed  CAS  Google Scholar 

  • Saito-Nakano Y, Yasuda T, Nakada-Tsukui K, Leippe M, Nozaki T (2004) Rab5-associated vacuoles play a unique role in phagocytosis of the enteric protozoan parasite Entamoeba histolytica. J Biol Chem 279:49497–49507

    Article  PubMed  CAS  Google Scholar 

  • Saito-Nakano Y, Loftus BJ, Hall N, Nozaki T (2005) The diversity of Rab GTPases in Entamoeba histolytica. Exp Parasitol 110:244–252

    Article  PubMed  CAS  Google Scholar 

  • Saito-Nakano Y, Mitra BN, Nakada-Tsukui K, Sato D, Nozaki T (2007) Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiol 9:1796–1808

    Article  PubMed  CAS  Google Scholar 

  • Sanchez MA, Peattie DA, Wirth D, Orozco E (1994) Cloning, genomic organization and transcription of the Entamoeba histolytica α-tubulin gene. Gene 146:239–244

    Article  PubMed  CAS  Google Scholar 

  • Sanchez-Lopez R, Gama-Castro S, Ramos MA, Merino E, Lizardi PM, Alagón A (1998) Cloning and expression of the Entamoeba histolytica ERD2 gene. Mol Biochem Parasitol 92:355–359

    Article  PubMed  CAS  Google Scholar 

  • Sanderfoot AA, Assaad FF, Raikhel NV (2000) The Arabidopsis genome. An abundance of soluble N-ethylmaleimide-sensitive factor adaptor protein receptors. Plant Physiol 124:1558–1569

    Article  PubMed  CAS  Google Scholar 

  • Schulter OM, Khvotchev M, Jahn R, Sudhof TC (2002) Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion. J Biol Chem 277(43):40919–40929

    Article  CAS  Google Scholar 

  • Scott CC, Dobson W, Botelho RJ, Coady-Osberg N, Chavrier P, Knecht DA, Heath C, Stahl P, Grinstein S (2005) Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis. J Cell Biol 169:139–149

    Article  PubMed  CAS  Google Scholar 

  • Seaman MN (2004) Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer. J Cell Biol 165:111–122

    Article  PubMed  CAS  Google Scholar 

  • Seaman NM (2005) Recycle your receptors with retromer. Trends Cell Biol 15:68–75

    Article  PubMed  CAS  Google Scholar 

  • Seigneur M, Mounier J, Prevost MC, Guillén N (2005) A lysine- and glutamic acid-rich protein, KERP1, from Entamoeba histolytica binds to human enterocytes. Cell Microbiol 7:569–579

    Article  PubMed  CAS  Google Scholar 

  • Serrano-Luna JJ, Negrete E, Reyes M, de la Garza M (1998) Entamoeba histolytica HM1:IMSS: hemoglobin-degrading neutral cysteine proteases. Exp Parasitol 89:71–77

    Article  PubMed  CAS  Google Scholar 

  • Silverstein SC, Steinman RM, Cohn ZA (1977) Endocytosis. Annu Rev Biochem 46:669–722

    Article  PubMed  CAS  Google Scholar 

  • Singer-Krüger B, Stenmark H, Düsterhöft A, Philippsen P, Yoo JS, Gallwitz D, Zerial M (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125:283–298

    Article  PubMed  Google Scholar 

  • Slapeta J, Keithly JS (2004) Cryptosporidium parvum mitochondrial-type HSP70 targets homologous and heterologous mitochondria. Eukaryot Cell 3:483–494

    Article  PubMed  CAS  Google Scholar 

  • Stanley SL Jr (2003) Amoebiasis. Lancet 361:1025–1034

    Article  PubMed  CAS  Google Scholar 

  • Stenmark H, Aasland R (1999) FYVE-finger proteins-effectors of an inositol lipid. J Cell Sci 112:4175–4183

    PubMed  CAS  Google Scholar 

  • Stenmark H, Olkkonen VM (2001) The Rab GTPase family. Genome Biol 2:REVIEWS3007

    Google Scholar 

  • Stenmark H, Parton RG, Steele-Mortimer O, Lütcke A, Gruenberg J, Zerial M (1994) Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO J 13:1287–1296

    PubMed  CAS  Google Scholar 

  • Stenmark H, Aasland R, Driscoll PC (2002) The phosphatidylinositol 3-phosphate-binding FYVE finger. FEBS Lett 513:77–84

    Article  PubMed  CAS  Google Scholar 

  • Stinchcombe JC, Barral DC, Mules EH, Booth S, Hume AN, Machesky LM, Seabra MC, Griffiths GM (2001) Rab27a is required for regulated secretion in cytotoxic T lymphocytes. J Cell Biol 152:825–834

    Article  PubMed  CAS  Google Scholar 

  • Sutak R, Dolezal P, Fiumera HL, Hrdy I, Dancis A, Delgadillo-Correa M, Johnson PJ, Müller M, Tachezy J (2004) Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis. PNAS 101:10368–10373

    Article  PubMed  CAS  Google Scholar 

  • Tamaki TH, Yamashina S (2002) Structural integrity of the Golgi stack is essential for normal secretory functions of rat parotid acinar cells: effects of brefeldin A and okadaic acid. J Histochem Cytochem 50:1611–1623

    Article  PubMed  CAS  Google Scholar 

  • Teixeira JE, Huston CD (2008) Participation of the serine-rich Entamoeba histolytica protein in amebic phagocytosis of apoptotic host cells. Infect Immun 76:959–966

    Article  PubMed  CAS  Google Scholar 

  • Teixeira JE, Heron BT, Huston CD (2008) C1q- and Collectin-dependent phagocytosis of apoptotic host cells by the intestinal protozoan Entamoeba histolytica. J Infect Dis 198:1062–1070

    Article  PubMed  CAS  Google Scholar 

  • Temesvari LA, Harris EN, Stanley SL, Cardelli JA (1999) Early and late endosomal compartments of Entamoeba histolytica are enriched in cysteine proteases acid phosphatase and several Ras-related Rab GTPases. Mol Biochem Parasitol 103:225–241

    Article  PubMed  CAS  Google Scholar 

  • Tovar J, Fischer A, Clark CG (1999) The mitosome, a novel organelle related to mitochondria in the amitochondrial parasite Entamoeba histolytica. Mol Microbiol 32:1013–1021

    Article  PubMed  CAS  Google Scholar 

  • Tovar J, León-Avila G, Sánchez LB, Sutak R, Tachezy J, van der Giezen M, Hernández M, Muller M, Lucocq JM (2003) Mitochondrial remnant organelles of Giardia function in iron-sulfur protein maturation. Nature 426:172–176

    Article  PubMed  CAS  Google Scholar 

  • Tovar J, Cox SSE, van der Giezen M (2007) A mitosome purification protocol based on percoll density gradients and its use in validating the mitosomal nature of E. histolytica mitochondrial Hsp70. Methods Mol Biol 390:167–177

    Article  PubMed  CAS  Google Scholar 

  • Trombetta ES, Parodi AJ (2003) Quality control and protein folding in the secretory pathway. Annu Rev Cell Dev Biol 19:649–676

    Article  PubMed  CAS  Google Scholar 

  • Tsaousis AD, Kunji ER, Goldberg AV, Lucocq JM, Hirt RP, Embley TM (2008) A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi. Nature 453:553–556

    Article  PubMed  CAS  Google Scholar 

  • Ueda T, Nakano A (2002) Vesicular traffic: an integral part of plant life. Curr Opin Plant Biol 5:513–517

    Article  PubMed  CAS  Google Scholar 

  • Ungermann C, Langosch D (2005) Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixing. J Cell Sci 118:3819–3828

    Article  PubMed  CAS  Google Scholar 

  • Ungermann C, Sato K, Wickner W (1998) Defining the functions of trans-SNARE pairs. Nature 365:543–548

    Article  CAS  Google Scholar 

  • Ungermann C, Price A, Wickner W (2000) A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of docking. PNAS 97:8889–8891

    Article  PubMed  CAS  Google Scholar 

  • van der Giezen M, Cox S, Tovar J (2004) The iron-sulfur cluster assembly genes iscS and iscU of Entamoeba histolytica were acquired by horizontal gene transfer. BMC Evol Biol 4:7

    Article  PubMed  Google Scholar 

  • van der Giezen M, León-Avila G, Tovar J (2005) Characterization of chaperonin 10 (Cpn10) from the intestinal human pathogen Entamoeba histolytica. Microbiology 151:3107–3115

    Article  PubMed  CAS  Google Scholar 

  • Vats D, Vishwakarma RA, Bhattacharya S, Bhattacharya A (2005) Reduction of cell surface glycosylphosphatidylinositol conjugates in Entamoeba histolytica by antisense blocking of E. histolytica GlcNAc-phosphatidylinositol deacetylase expression: effect on cell proliferation, endocytosis, and adhesion to target cells. Infect Immun 73:8381–8392

    Article  PubMed  CAS  Google Scholar 

  • Vayssié L, Vargas M, Weber C, Guillén N (2004) Double-stranded RNA mediates homology-dependent gene silencing of γ-tubulin in the human parasite Entamoeba histolytica. Mol Biochem Parasitol 138:21–28

    Article  PubMed  CAS  Google Scholar 

  • Vieira OV, Harrison RE, Scott CC, Stenmark H, Alexander D, Liu J, Gruenberg J, Schreiber AD, Grinstein S (2004) Acquisition of Hrs, an essential component of phagosomal maturation, is impaired by mycobacteria. Mol Cell Biol 24:4593–4604

    Article  PubMed  CAS  Google Scholar 

  • Voellmy R, Boellmann F (2007) Chaperone regulation of the heat shock protein response. Adv Exp Med Biol 594:89–99

    Article  PubMed  Google Scholar 

  • Voigt H, Guillén N (1999) New insights into the role of the cytoskeleton in phagocytosis of Entamoeba histolytica. Cell Microbiol 1:195–203

    Article  Google Scholar 

  • Voigt H, Olivo JC, Sansonetti P, Guillén N (1999) Myosin IB from Entamoeba histolytica is involved in phagocytosis of human erythrocytes. J Cell Sci 112:1191–1201

    PubMed  CAS  Google Scholar 

  • Weber C, Blazquez S, Marion S, Ausseur C, Vats D, Krzeminski M, Rigothier MC, Maroun RC, Bhattacharya A, Guillén N (2008) Bioinformatics and functional analysis of an Entamoeba histolytica mannosyltransferase necessary for parasite complement resistance and hepatical infection. PLoS Negl Trop Dis 2:e165

    Article  PubMed  CAS  Google Scholar 

  • Welter BH, Laughlin RC, Temesvari LA (2002) Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica. Mol Biochem Parasitol 121:254–264

    Article  PubMed  CAS  Google Scholar 

  • Welter BH, Powell RR, Leo M, Smith CM, Temesvari LA (2005) A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells. Mol Biochem Parasitol 140:161–173

    Article  PubMed  CAS  Google Scholar 

  • Welter BH, Powell RR, Laughlin RC, McGugan GC, Bonner M, King A, Temesvari LA (2006) Entamoeba histolytica: comparison of the role of receptors and filamentous actin among various endocytic processes. Exp Parasitol 113:91–99

    Article  PubMed  CAS  Google Scholar 

  • Williams BA, Hirt RP, Lucocq JM, Embley TM (2002) A mitochondrial remnant in the microsporidian Trachipleistophora hominis. Nature 418:865–869

    Article  PubMed  CAS  Google Scholar 

  • Yeung T, Ozdamar B, Paroutis P, Grinstein S (2006) Lipid metabolism and dynamics during phagocytosis. Curr Opin Cell Biol 18:429–437

    Article  PubMed  CAS  Google Scholar 

  • Yi Y, Samuelson J (1994) Primary structure of the Entamoeba histolytica gene (EhvmaI) encoding the catalytic peptide of a putative vacuolar membrane proton-transporting ATPase (V-ATPase). Mol Biochem Parasitol 66:165–169

    Article  PubMed  CAS  Google Scholar 

  • Zamarripa-Morales S, Villagómez-Castro JC, Calvo-Méndez C, Flores-Carreón A, López-Romero E (1999) Entamoeba histolytica: identification and properties of membrane-bound and soluble alpha-glucosidases. Exp Parasitol 93:109–115

    Article  PubMed  CAS  Google Scholar 

  • Zerial M, McBride H (2001) Rab protein as membrane organizers. Nat Rev Mol Cell Biol 2:107–117

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The work of the Unité Biologie Cellulaire du Parasitisme (BCP unit) is sponsored in part by the Pasteur–Weizmann Research Council and the French granting agency, ANR. Sherri Smith is supported by a grant from the Pasteur Foundation of New York.

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  1. Institut Pasteur, Cell Biology of Parasitism Unit, 25–28 rue du Docteur Roux, 75015, Paris, France

    Sherri S. Smith & Nancy Guillen

  2. Inserm U786, 25–28 rue du Docteur Roux, 75015, Paris, France

    Sherri S. Smith & Nancy Guillen

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    W. de Souza

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Smith, S.S., Guillen, N. (2010). Organelles and Trafficking in Entamoeba histolytica . In: de Souza, W. (eds) Structures and Organelles in Pathogenic Protists. Microbiology Monographs, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12863-9_7

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