Abstract
With the increasing rate of fungal infection, it is important for clinical mycologists to know the details of classical and newer molecular approaches in diagnosis of fungal infection. The classical approach includes appropriate specimen collection and transport, as fungus isolation is not so easy as for bacteriological samples. Direct microscopic examination with 10% KOH and Calcofluor white staining plays a very important role, as it helps to provide a presumptive diagnosis to the physician and also permits the selection of appropriate culture media. The fungal culture media used are always two, one being a non-selective medium and the other with addition of antibiotics and cycloheximide, thus reducing bacterial and saprobic fungal contamination. To obtain a better fungal morphology, slide culture is usually done. There are certain differential test media to identify the different species of fungi. As the media for culture are available in plates or tubes, with their respective advantages and disadvantages, their use depends on the preference of the laboratory concerned. As we all know that fungi causing disease are either yeasts, molds or dimorphic, hence the culture media is incubated at two different temperatures of 30°C (for the mold form of all fungi) and 35°C (for yeast forms of dimorphic fungi). As it takes a long time for fungi to grow, they should be incubated for at least 30 days before giving a negative culture report. Fungal serology is currently performed only in selected reference laboratories. The test is rapid, and aids in diagnosis of systemic fungal infection, and also helps in the study of epidemiology of the fungal infection. The commonly used serological tests are ELISA, EIA, immunodiffusion and latex agglutination test, with commercially available kits. Now with the advent of molecular techniques including PCR and DNA probes, identification of fungal infection has become even more sensitive and specific. It is rapid, and can detect fungi even in culture plates contaminated with bacteria or other saprobic fungi. In this review, we will describe in detail the important clinical aspects in the laboratory diagnosis of fungal infections, and hope that it will be a reference chapter for beginners as well as for established researchers working in the current field.
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References
Ahmad S, Khan Z, Mustafa AS, Khan ZU (2002) Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection and biochemical methods for species identification. J Clin Microbiol 40:2483–2489
Alexander BD (2002) Diagnosis of fungal infection: new technologies for the mycology laboratory. Transpl Infect Dis 4(Suppl 3):32–37
Aly R (1994) Culture media for growing dermatophytes. J Am Acad Dermatol 31:S107–S108
Ansborg R, vov Heinegg EH, Rath PM (1994) Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients. Eur J Clin Microbiol Infect Dis 13:582–589
Barnes AJ (1993) Aspergillus infection: does serodiagnosis work? Serodiagn Immunother Infect Dis 5:135–138
Bialek R, Feucht A, Aepinus C, Just-Nubling G, Robertson VJ, Knobloch J, Hohle R (2002) Evaluation of two nested PCR assays for detection of Histoplasma capsulatum DNA in human tissue. J Clin Microbiol 40:1644–1647
Bialek R, Cirera AC, Herrmann T, Aepinus C, Shearn-Bochsler VI, Legendre AM (2003) Nested PCR assays for detection of Blastomyces dermatitidis DNA in paraffin-embedded canine tissue. J Clin Microbiol 41:205–208
Bille J, Stockman L, Roberts GD, Horstmeier CD, Ilstrup DM (1983) Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. J Clin Microbiol 18:469–471
Bille J, Edson RS, Roberts GD (1984) Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system. J Clin Microbiol 19:126
Buchheidt D, Spiess B, Hehlmann R (2001) Systemic infections with Aspergillus species in patients with hematological malignancies: current serologic and molecular diagnostic approaches. Onkologie 24:531–536
Buchheidt D, Baust C, Skladny H, Baldus M, Brauninger S, Hehlmann R (2002) Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus. Br J Haematol 116:803–811
Burgener-Kairuz P, Zuber JP, Jaunin P, Buchman TG, Bille J, Rossier M (1994) Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species–species nested PCR amplification of a cytochrome P-450 Lanosterol-alpha-demethylase (L1A1) gene fragment. J Clin Microbiol 32:1902–1907
Campbell CC, Hill GB (1964) Further studies on the development of complement-fixing antibodies and precipitins in healthy histoplasmin-sensitive persons following a single histoplasmin skin test. Am Rev Respir Dis 90:927–934
Cao L, Chen DL, Lee C, Chan CM, Chan KM, Vanittanakom N, Tsang DN, Yuen KY (1998) Detection of specific antibodies to an antigenic mannoprotein for diagnosis of Penicillium marneffei penicilliosis patients. J Clin Microbiol 36:3028–3031
Cao L, Chan KM, Chen D, Vanittanakom N, Lee C, Cham CM, Sirisanthana T, Tsang DN, Yuen KY (1999) Detection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patients. J Clin Microbiol 37:981–986
Chandler FW, Kaplan W, Ajello L (1989) A colour atlas and textbook of the histopathology of mycotic diseases. Wolfe Medical, London
Chang HC, Leaw SN, Huang AH, Wu TL, Chang TC (2001) Rapid identification of yeast in positive blood cultures by a Multiplex PCR method. J Clin Microbiol 39:3466–3471
Chen SCA, Halliday CL, Meyer W (2002) A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Med Mycol 40:333–357
Chryssanthou E, Klingspor L, Tollemar J, Petrini B, Larsson L, Christensson B, Ringden O (1999) PCR and other non-culture methods for diagnosis of invasive candida infections in allogenic bone marrow and solid organ transplant recipients. Mycoses 42:239–247
Clayton YM, Midgley G (1989) Identification of agents of superficial mycoses. In: Evans EGV, Richardson MD (eds) Medical Mycology-a practical approach. IRL at Oxford University Press, Oxford, pp 65–95
Conner DH, Chandler FW, Schwartz DA, Manz HJ, Lack EE (1997) Pathology of infectious diseases. Appleton and Lange, Stamford, CT
De Bernardis F, Girmenia C, Boccanera M, Adriani D, Martino P, Cassone A (1993) Use of a monoclonal antibody in a dot immunobinding assay for detection of a circulating mannoprotein of Candida spp., in neutropenic patients with invasive candidiasis. J Clin Microbiol 31:3142–3146
de Hoog GS, Guarro J, Gene J, Figueras MJ (eds) (2000) Ascomycetous yeasts genus: Candida. In: Atlas of Clinical Fungi, 2nd edn. Centtraalbureau voor Schimmelcultures, Delft, The Netherlands, pp 180–226
Delvenne P, Arrese JE, Thiry A, Borlee-Hermans G, Pierard GE, Boniver J (1993) Detection of cytomegalovirus, Pneumocystis carinii and Aspergillus species in bronchoalveolar fluid — a comparison of techniques. Am J Clin Pathol 100:414–418
Di Salvo AF, Terreni AA, Wooten AK (1981) Use of the exoantigen test to identify Blastomyces dermitiidis, Coccidioides immitis and Histoplasma capsulatum in mixed cultures. Am J Clin Pathol 75:825–826
Durkin MM, Connolly PA, Wheat LJ (1997) Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum antigen. J Clin Microbiol 35:2252–2255
Edwards LB, Acquaviva FA, Livesay VT, Cross FW, Palmer CE (1969) An atlas of sensitivity to tuberculin, PPD-B and histoplasmin in the United States. Am Rev Respir Dis 99:1–132
Ferns RB, Fletcher H, Bradley S, Mackinnon S, Hunt C, Tedder RS (2002) The prospective evaluation of a nested polymerase chain reaction assay for the early diagnosis of Aspergillus infection in patients with leukemia or undergoing allograft treatment. Br J Haematol 119:720–725
Ferreira RP, Yu B, Niki Y, Armstrong D (1990) Detection of Candida antigenuria in disseminated candidiasis by immunoblotting. J Clin Microbiol 28:1075–1078
Fischer JB, Kane J (1974) The laboratory diagnosis of dermatophytosis complicated by Candida albicans. Can J Microbiol 20:167–182
Flahaut M, Sanglard D, Monod M, Bille J, Rossier M (1998) Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C. albicans-secreted aspartic proteinase genes. J Clin Microbiol 36:395–401
Fratamico PM, Buckley HR (1991) Identification and characterization of an immunodominant 58-kilodalton antigen of Aspergillus fumigatus recognized by sera of patients with invasive aspergillosis. Infect Immun 59:309–315
Fujita S, Hashimoto T (1992) Detection of serum Candida antigens by enzyme-linked immunosorbent assay and a latex agglutination test with anti-Candida albicans and anti-Candida krusei antibodies. J Clin Microbiol 30:3132–3137
Fuller DD, Davis TE Jr, Denys GA, York MK (2001) Evaluation of BACTEC MYCO/F Lytic medium for recovery of mycobacteria, fungi, and bacteria from blood. J Clin Microbiol 39:2933–2936
Gade W, Ledman DW, Wethington R, Yi A (1992) Serologic responses to various Coccidioides antigen preparations in a new enzyme immunoassay. J Clin Microbiol 30:1907–1912
Geha DJ, Roberts GD (1994) Laboratory detection of fungemia. Clin Lab Med 14:83–97
Gomes GM, Cisalpino PS, Taborda CP, de Camargo ZP (2000) PCR for diagnosis of paracoccidioidomycosis. J Clin Microbiol 38:3478–3480
Graser Y, de Hoog GS, Kuijpers AFA (2000a) Recent advances in the molecular taxonomy of dermatophytes. In: Kushwaha RKS, Guarro J (eds) Biology of dermatophytes and other keratinophilic fungi. Revista Iberoamericana de Micologia, Bilbao, Spain, pp 17–21
Graser Y, Kuijpers AFA, Presber W, de Hoog GS (2000b) Molecular taxonomy of Trichophyton rubrum complex. J Clin Microbiol 38:3329–3336
Green F, Balish E (1980) Trichophyton mentagrophytes dermatophytosis in germfree guinea pigs. J Invest Dermatol 75:476–480
Grossman J, Baum J, Gluckman J, Fusner J, Condemi JJ (1975) The effect of ageing and acute illness on delayed hypersensitivity. J Allergy Clin Immunol 55:268–275
Hageage G, Harrington B (1984) Use of calcofluor white in clinical mycology. Lab Med 15:109
Hariri ALR, Hemple HO, Kimberlin CL, Goodman NL (1982) Effects of time elapse between sputum collection and culturing on isolation of clinically significant fungi. J Clin Microbiol 15:425–428
Haynes KA, Westerneng TJ, Fell JW, Moens W (1995) Rapid detection and identification of pathogenic fungi by polymerase chain reaction amplification of large subunit ribosomal DNA. J Med Vet Mycol 33:319–325
Hazen KC, Howell SA (2003) Candida, Cryptococcus, and other yeasts of medical importance. In: Murray PR, Baron EJ et al (eds) Manual of Clinical Microbiology, 8th edn. ASM, Washington DC, pp 1693–1711
Hopwood V, Evans EGV (1991) Serological tests in the diagnosis and prognosis of fungal infection in the compromised patient. In: Warnock DW, Richardson MD (eds) Fungal infection in the compromised patient, 2nd edn. Wiley, Chichester, pp 311–353
Hopwood V, Johnson EM, Cornish JM, Foot AB, Evans EG, Warnock DW (1995) Use of Pastorex Aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis. J Clin Pathol 48:210–213
Hossain MA, Miyazaki T, Mitsutake K, Kakeya H, Yamamoto Y, Yanagihara K, Kawamura S, Otsubo T, Hirakata Y, Tashiro T, Kohno S (1997) Comparison between Wako-WB003 and Fungitech G tests for detection of (1, 3)-beta-D-glucan in systemic mycoses. J Clin Lab Anal 11:73–77
Johnson SM, Pappagianis D (1992) The coccidioidal complement fixation and immunodiffusion-complement fixation antigen is a chitinase. Infect Immun 60:2588–2592
Jones DB, Liesegang TJ, Robinson NM (1981) Cumitech 13. American Society for Microbiology, Washington, DC
Jordan JA (1994) PCR identification of four medically important Candida species by using a single primer pair. J Clin Microbiol 32:2962–2967
Kaminski GW (1985) The routine use of modified Borelli’s lactritmel (MBLA). Mycopathologia 91:57–59
Kane J, Fischer JB (1971) The differentiation of Trichophyton rubrum and T. mentagrophytes by use of Christensen’s urea broth. Can J Microbiol 17:911–913
Kane J, Fischer JB (1975) The influence of sodium chloride on the growth and morphology of dermatophytes and some other keratinophilic fungi. Can J Microbiol 21:742–749
Kane J, Smitka CM (1978) Early detection and identification of Trichophyton verrucosum. J Clin Microbiol 8:740–747
Kane J, Salkin IF, Weitzman I, Smitka C (1981) Trichophyton raubitschekii sp. nov. Mycotaxon 13:259–266
Kaplan W, Kaufman L (1963) Specific fluorescent antiglobulins for the detection and identification of Blastomyces dermititidis yeast-phase cells. Mycopathologia 19:173–180
Kappe R, Seeliger HPR (1993) Serodiagnosis of deep-seated fungal infection. Curr Top Med Mycol 5:247–280
Kappe R, Okeke CN, Fauser C, Maiwald M, Sonntag HG (1998) Molecular probes for the detection of pathogenic fungi in the presence of human tissue. J Med Microbiol 47:811–820
Kaufman L, Standard P (1978) Improved version of the exoantigen test for identification of Histoplasma capsulatum. J Clin Microbiol 8:42–45
Kaufman L, Kovacs JA, Reiss E (1997) Clinical Immunomycology. American Society for Microbiology, Washington, DC
Kaufman L, Standard PG, Weeks RJ, Padhye AA (1983) Detection of two Blastomyces dermititidis serotypes by exoantigen analysis. J Clin Microbiol 18:110–114
Kaufman L, Standard PG, Anderson SA, Jalbert M, Swisher BL (1995) Development of specific fluorescent-antibody test for tissue form of Penicillium marneffei. J Clin Microbiol 33:2136–2138
Kaufman L, Standard PG, Jalbert M, Kantipong P, Limpakarnjanarat K, Mastro TD (1996) Diagnostic antigenemia tests for penicilliosis marneffei. J Clin Microbiol 34:2503–2505
Kawazu M, Kanda Y, Goyama S, Takeshita M, Nannya Y, Niino M, Komeno Y, Nakamoto T, Kurokawa M, Tsujino S, Ogawa S, Aoki K, Chiba S, Motokura T, Ohishi N, Hirai H (2003) Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage fluid. Am J Hematol 72:27–30
Kiehn TE, Bernard EM (1979) Candidiasis: detection by gas liquid chromatography of D-arabinitol, a fungal metabolite, in human serum. Science 206:577–580
Kitch TT, Jacobs MR, McGinnis MR, Appelbaum PC (1996) Ability of Rapid Yeast Plus system to identify 304 clinically significant yeasts within 5 hours. J Clin Microbiol 34:1069–1071
Klein BS, Jones JM (1990) Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermititidis yeasts to detect antibody in infected patients. J Clin Invest 85:152–161
Kruse D, Cole GT (1992) A seroactive 120-kilodalton β-1, 3-glucanase of Coccidioides immitis which may precipitate in spherule-morphogenesis. Infect Immun 60:4350–4363
Kwon-Chung J, Bennett J (1992) Medical Mycology. Lea and Febiger, Philadelphia, PA
Lacaz CS, Porto E, Martins JEC, Heins-Vaccari EM, Melo NT (2002) Paracoccidioidomicose. In: Lacaz CS, Porto E et al (eds) Micologia medica, 9th edn. Sarvier, Sao Paulo, Brasil, pp 629–729
Land GA, Salkin IF, el-Zaatari M, McGinnis MR, Hashem G (1991) Evaluation of Baxter-Microscan 4-hour enzyme-based yeast identification system. J Clin Microbiol 29:718–722
Larone DH (1995) Medically Important Fungi, 3rd edn. American Society of Microbiology, Washington, DC
Lass-Florl C, Rath PM, Niederwieser D, Kofler G, Wurzner R, Krezy A, Dierich MP (2000) Aspergillus terreus infections in hematological malignancies: molecular epidemiology suggest association with in-hospital patients. J Hosp Infect 46:31–35
Lindsley MD, Hurst SF, Iqbal NJ, Morrison CJ (2001) Rapid identification of dimorphic and yeast-like fungal pathogens using specific DNA probes. J Clin Microbiol 39:3505–3511
Liu D, Coloe S, Baird R, Pedersen J (2000) Application of PCR to the identification of dermatophyte fungi. J Med Microbiol 49:493–497
LoBuglio KF, Taylor JW (1995) Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei. J Clin Microbiol 33:85–89
Loeffler J, Hagmeyer L, Hebart H, Henke N, Schumacher U, Einsele H (2000a) Rapid detection of point mutations by fluorescence resonance energy transfer and probe melting curves in Candida species. Clin Chem 46:631–635
Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, Einsele H (2000b) Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J Clin Microbiol 38:586–590
Maiwald M, Kappe R, Sonntag HG (1994) Rapid presumptive identification of medically relevant yeasts to the species level b polymerase chain reaction and restriction enzyme analysis. J Med Vet Mycol 32:115–122
Manso E, Montillo M, De Sio G, D’Amico S, Discepoli G, Leoni P (1994) Value of antigen and antibody detection in the serological diagnosis of invasive aspergillosis in patients with hematological malignancies. Eur J Clin Microbiol Infect Dis 13:756–760
Martagon-Villamil J, Shreshtha N, Sholtis M, Isada CM, Hall GS, Bryne T, Lodge BA, Reller LB, Procop GW (2003) Identification of Histoplasma capsulatum from culture extracts by real-time PCR. J Clin Microbiol 41:1295–1298
Martins R, Marques S, Alves M, Fecchio D, deFranco MF (1997) Serological follow-up of patients with paracoccidioidomycosis treated with itraconazole using Dot-blot, ELISA and western-blot. Rev Inst Med Trop Sao Paulo 39:261–269
McGinnis MR (1980) Laboratory Handbook of Medical Mycology. Academic, New York
Mendes-Giannini MJ, Camargo ME, Lacaz CS, Ferreira AW (1984) Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. J Clin Microbiol 20:103–108
Mendes-Giannini MJS, Del Negro GB, Siqueira AM (1994) Serodiagnosis. In: Franco M, Lacaz CS et al (eds) Paracoccidioidomycosis. CRC, Boca Raton, FL, pp 345–363
Merz W, Roberts G (1995) Detection and recovery of fungi from clinical specimens. In: Murray P et al (eds) Manual of Clinical Microbiology. ASM, Washington, DC
Merz WG, Roberts GD (2003a) Algorithms for detection and identification of fungi. In: Murray PR, Baron EJ et al (eds) Manual of Clinical Microbiology, 8th edn. ASM, Washington, DC, pp 668–685
Merz WG, Roberts GD (2003b) Detection and recovery of fungi from clinical specimens. American Society for Microbiology, Washington, DC
Merz WG, Berger CL, Silva-Hutner M (1970) Media with pH indicators for the isolation of dermatophytes. Arch Dermatol 102:545–547
Milne LJR, Barnetson RStC (1974) Diagnosis of dermatophytoses using vinyl adhesive tape. Sabouraudia 12:162–165
Mitsutake K, Kohno S, Miyazaki T, Miyazaki H, Maesaki S, Koga H (1994) Detection of Candida enolase antibody in patients with candidiasis. J Clin Lab Anal 8:207–210
Miyazaki T, Kohno S, Mitsutake K, Maesaki S, Tanaka K, Ishikawa N, Hara K (1995) Plasma (1→3)-beta-D-glucan and fungal antigenemia in patients with candidemia, aspergillosis, and cptococcosis. J Clin Microbiol 33:3115–3118
Moriello KA, Deboer DJ (1991) Fungal flora of haircoat of cats with and without dermatophytosis. J Med Vet Mycol 29:285–292
Munoz P, Alcala L, Sanchez Conde M, Palomo J, Yanez J, Pelaez T, Bouza E (2003) The isolation of Aspergillus fumigatus from respiratory tract specimens in heart transplant recipients is highly predictive of invasive aspergillosis. Transplantation 75:326–329
Nalesnik MA, Myerowitz RL, Jenkins R, Lenkey J, Herbert D (1980) Significance of Aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis. J Clin Microbiol 11:370–376
Niesters HG, Goessens WH, Meis JF, Quint WG (1993) Rapid, polymerase chain reaction based identification assays for Candida species. J Clin Microbiol 31:904–910
Obayashi T, Yoshida M, Mori T, Goto H, Yasuoka A, Iwasaki H, Teshima H, Kohno S, Horiuchi A, Ito A, Yamaguchi H, Shimada K, Kawai T (1995) Plasma (1-3)-β-d-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes. Lancet 345:17–20
Odds FC, Bernaerts RIA (1994) CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:1923–1929
Poulain D, Slomianny C, Jouault T, Gomez JM, Trinel PA (2002) Contribution of phopholipomannan to the surface expression of beta-1, 2-oligimannosides in Candida albicans and its presence in cell wall extracts. Infect Immun 70:4323–4328
Rebell G, Taplin D (1974) Dermatophytes, their recognition identification. University of Miami Press, Coral Gables, FL
Reboli AC (1993) Diagnosis of invasive candidiasis by a dot immunobinding assay for Candida antigen detection. J Clin Microbiol 31:518–523
Reddy LV, Kumar A, Kurup VP (1993) Specific amplification of Aspergillus fumigatus DNA by polymerase chain reaction. Mol Cell Probes 7:121–126
Reiss E, Morrison CJ (1993) Nonculture methods for diagnosis of disseminated candidiasis. Clin Microbiol Rev 6:311–323
Reiss E, Kaufman L, Kovacs JA, Lindsley MD (2002) Clinical immunomycology. In: Rose NR, Hamilton RG, Detrick B (eds) Manual of Clinical Laboratory Immunology. ASM, Washington, DC, pp 559–583
Resnick S, Pappagianis D, McKerrow JH (1987) Proteinase production by the parasitic cycle of the pathogenic fungus Coccidioides immitis. Infect Immun 55:2807–2815
Rickerts V, Bialek R, Tintelnot K, Jacobi V, Just-Nubling G (2002) Rapid PCR-based diagnosis of disseminated histoplasmosis in an AIDS patient. Eur J Clin Microbiol Infect Dis 21:821–823
Rippon JW (1988) Medical Mycology, the Pathogenic fungi and Pathogenic Actionmycetes, 3rd edn., WB Saunders, Philadelphia
Roberts GD (1975) Detection of fungi in clinical specimens by phase-contrast microscopy. J Clin Microbiol 2:261
Salkin IF (1973) Dermatophyte test medium: evaluation with non-dermatophyte pathogens. Appl Microbiol 26:134–137
Sandhu GS, Kline BC, Stockman L, Roberts GD (1995) Molecular probes for diagnosis of fungal infections. J Clin Microbiol 33:2913–2919
Sarosi GA, Armstrong D, Davies SF, George RB, Graybill JR, Pennington JE et al (1985) Laboratory diagnosis of mycotic and specific fungal infections. Am Rev Respir Dis 132:1373–1379
Sekhon AS, Li JSK, Gag AK (1982) Penicillosis marneffei: serological and exoantigen studies. Mycopathologia 77:51–57
Sendid B, Poirot JL, Tabouret M, Bonnin A, Caillot D, Camus D, Poulain D (2002) Combined detection of mannanaemia and anti mannan antibodies as a strategy for the diagnosis of systemic infection caused by pathogenic Candida species. J Med Microbiol 51:433–442
Smith CE, Saito MT, Simons SA (1956) Pattern of 39, 500 serological tests in coccidioidomycosis. J Am Med Assoc 160:546–552
St Germain G, Beauchesne D (1991) Evaluation of the MicroScan Rapid Yeast Identification panel. J Clin Microbiol 29:2296–2299
St-Germain G, Summerbell RC (1996) Identifying filamentous fungi: a clinical laboratory handbook. Star Publishing Co., Belmont CA
Stockman L, Clark KA, Hunt JM, Roberts JD (1993) Evaluation of commercially available acridinium ester-labeled chemiluminescent DNA probes for culture identification of Blastomyces drematitidis, Coccidioides immitis, Cryptococcus neoformans and Histoplasma capsulatum. J Clin Microbiol 31:845–850
Suarez SM, Silvers DN, Scher RK, Pearlstein HH, Auerbach R (1991) Histologic evaluation of nail clippings for diagnosing onychomycosis. Arch Dermatol 127:1517–1519
Supparatpinyo K, Sirisanthana T (1994) Disseminated Penicillium marneffei infection diagnosed on examination of a peripheral blood smear of a patient with human immunodeficiency virus infection. Clin Infect Dis 18:246–247
Supparatpinyo K, Khamwan C, Baosoung V, Nelson KE, Sirisanthana T (1994) Disseminated Penicillium marneffei infection in Southeast Asia. Lancet 344:110–113
Switchenko AC, Miyada CG, Goodman TC, Walsh TJ, Wong B, Becker MJ, Ullman EF (1994) An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species. J Clin Microbiol 32:92–97
Taborda CP, Camargo ZP (1993) Diagnosis of paracoccidioidomycosis by passive haemagglutination assay of antibody using a purified and specific antigen-gp43. J Med Vet Mycol 31:155–160
Taborda CP, Camargo ZP (1994) Diagnosis of paracoccidioidomycosis by dot immunobinding assay for antibody detection using the purified and specific antigen-gp43. J Clin Microbiol 32:554–556
Telenti A, Roberts GD (1989) Laboratory detection of fungemia. J Clin Microbiol 8:825–831
Turenne CY, Sanche SE, Hoban DJ, Karlowsky JA, Kabani AM (1999) Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J Clin Microbiol 37:1846–1851
Turin L, Riva F, Galbiati G, Cainelli T (2000) Fast, simple and highly sensitive double-rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens. Eur J Clin Invest 30:511–518
Vanittanakom N, Vanittanakom P, Hay RJ (2002) Rapid identification of Penicillium marneffei by PCR-based detection of specific sequence on the rRNA gene. J Clin Microbiol 40:1739–1742
Verweij PE, Latge JP, Rijs AJ, Melchers WJ, BEDe P, Hoogkamp-Korstanje JA, Meis JF (1995a) Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for haematological malignancies. J Clin Microbiol 33:3150–3153
Verweij PE, Poulain D, Obayashi T, Patterson TF, Denning DW, Ponton J (1998) Current trends in detection of antigenemia, metabolites and cell wall markers for the diagnosis and therapeutic monitoring of fungal infections. Med Mycol 36(Suppl 1):146–155
Verweij PE, Rijs AJ, BEDe P, Horrevorts AM, Hoogkamp-Korstange JA, Meis JF (1995b) Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex agglutination test for diagnosing invasive aspergillosis. J Clin Pathol 48:474–476
Verweij PE, Stynen D, Rijs AJ, BEDe P, Hoogkamp-Korstanje JA, Meis JF (1995c) Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients. J Clin Microbiol 33:1912–1914
Vetter E, Torgerson C, Feuker A, Hughes J, Harmsen S, Schleck C, Horstmeier C, Roberts G, Cockerill F III (2001) Comparison of the BACTEC MYCO/F Lytic bottle to the isolator tube. BACTEC Plus Aerobic F/bottle, and BACTEC Anaerobic Lytic/10 bottle and comparison of the BACTEC Plus Aerobic F/bottle to the Isolator tube for recovery of bacteria, mycobacteria, and fungi from blood. J Clin Microbiol 39:4380–4386
Viviani MA, Tortorano AM, Rizzardini G, Quirino T, Kaufman L, Padhye AA, Ajello L (1993) Treatment and serological studies of an Italian case of penicilliosis marneffei contracted in Thailand by a drug addict infected with the human immunodeficiency virus. Eur J Epidemiol 9:79–85
Wahyuningsih R, Freisleben HJ, Sonntag HG, Schnitzler P (2000) Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J Clin Microbiol 38:3016–3021
Waite TR, Woods GL (1998) Evaluation of Myco Flytic medium for recovery of mycobacteria and fungi from blood. J Clin Microbiol 36:1176–1179
Walsh TJ, Chanock SJ (1997) Laboratory diagnosis of invasive candidiasis: a rationale for complementary use of culture and nonculture-based detection systems. Int J Infect Dis 1(Suppl 1):S11–S19
Walsh TJ, Francesconi A, Kasai M, Chanock SJ (1995) PCR and single-stranded conformational polymorphism for recognition of medically important fungi. J Clin Microbiol 33:3216–3220
Weissman Z, Berdicevsky I, Cavari B (1995) Molecular identification of Candida albicans. J Med Vet Mycol 33:205–207
Weitzman I, Kane J, Summerbell RC (1995) Trichophyton, microsporum, epidermophyton & agents of superficial mycoses. In: Murray PR, Baron EJ et al (eds) Manual of Clinical Microbiology, 6th edn. American Society for Microbiology, Washington, DC, pp 791–808
Wheat LJ (2003) Rapid diagnosis of invasive aspergillosis by antigen detection. Transpl Infect Dis 5:158–166
Wheat LJ, Kaufman CA (2003) Histoplasmosis. Infect Dis Clin North Am 17:1–19, vii
Wheat LJ, Connolly-Stringfield P, Williams B, Connolly K, Blair R, Bartlett M, Durkin M (1992) Diagnosis of histoplasmosis in patients with the acquired immunodeficiency syndrome by detection of Histoplasma capsulatum polysaccharide antigen in bronchoalveolar lavage fluid. Am Rev Respir Dis 145:1421–1424
Wheat J, French ML, Kohler RB, Zimmerman SE, Smith WR, Norton JA, Eitzen HE, Smith CD, Slama TG (1982) The diagnostic laboratory tests for histoplasmosis: analysis of experience in a large urban outbreak. Ann Intern Med 97:680–685
Wheat J, French ML, Kamel S, Tewari RP (1986a) Evaluation of cross-reactions in Histoplasma capsulatum serologic tests. J Clin Microbiol 23:493–499
Wheat LJ, Kohler RB, Tewari RP (1986b) Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med 314:83–88
Wickerham LJ, Burton KA (1948) Carbon assimilation tests for the classification of yeasts. J Bacteriol 56:363–371
Williams B, Fojtasek M, Connolly-Stringfield P, Wheat J (1994) Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, IN. Arch Pathol Lab Med 118:1205–1208
Yamakami Y, Hashimoto A, Tokimatsu I, Nasu M (1996) PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis. J Clin Microbiol 34:2464–2468
Yeo SF, Wong B (2002) Current status of nonculture methods for diagnosis of invasive fungal infections. Clin Microbiol Rev 15:465–484
Yuen KY, Wong SS, Tsang DN, Chau PY (1994) Serodiagnosis of Penicillium marneffei infection. Lancet 344:44–45
Zimmer BL, Pappagianis D (1989) Immunoaffinity isolation and partial characterization of the Coccidioides immitis antigen detected by tube precipitin and immunodiffusion-tube presipitin tests. J Clin Microbiol 27:1759–1766
Zimmer BL, Roberts GD (1979) Rapid selective urease test for presumptive identification of Cryptococcus neoformans. J Clin Microbiol 10:380–381
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Shahid, M., Ahmad, I., Malik, A., Jahan, N., Tripathi, T. (2010). Laboratory Diagnosis of Fungal Infections: An Overview. In: Ahmad, I., Owais, M., Shahid, M., Aqil, F. (eds) Combating Fungal Infections. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12173-9_9
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