Abstract
Fluorescence recovery after photobleaching (FRAP) is an established technique for determining solute diffusivity in biological tissues. Recently, we proposed a novel fluorescence photobleaching method, based on two independent analyses of the video-FRAP image series: the fast Fourier transform (FFT) and the Karhunen-Loève transform (KLT). In this study, the principles of this method are illustrated, and its optimization is discussed.
Computer simulated FRAP experiments were used for the optimization of the choice of frequencies used in FFT analysis. The sensitivity of the technique to image noise contamination was evaluated. Additionally, the method was experimentally validated by characterizing fluorescein diffusivity in human annulus fibrosus. The results were compared to diffusivity data obtained by other methods.
This study provides new insights into the optimization of data analysis of video-FRAP experiments. Furthermore, the experimental results verified the applicability of the method for the determination of solute diffusivity in biological media.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Travascio, F., Gu, W.Y. (2009). Novel Video-FRAP Method for the Determination of Anisotropic Diffusion: Principles and Optimization. In: McGoron, A.J., Li, CZ., Lin, WC. (eds) 25th Southern Biomedical Engineering Conference 2009, 15 – 17 May 2009, Miami, Florida, USA. IFMBE Proceedings, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01697-4_5
Download citation
DOI: https://doi.org/10.1007/978-3-642-01697-4_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-01696-7
Online ISBN: 978-3-642-01697-4
eBook Packages: EngineeringEngineering (R0)