Abstract
The study of enzymatic reaction mechanisms involves researching the elementary steps that intervene during the course of a reaction, and determining the rates of appearance and disappearance of reaction intermediates, as well as their relative energetic levels. The majority of intermediates that appear have very short lifetimes and are not generally detectable by classical techniques. Increasingly rapid methods have been progressively introduced for studying enzyme reactions as is the case more generally for all kinetic studies. The discovery of techniques that discern ever lower time constants marked great progress in the approaches taken to study enzymatic reaction mechanisms.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsBibliography
Books
Bernhard S. –1968– The structure and function of enzymes, Benjamin Inc., New York.
Chance B., Eisenhard R.H., Gibson Q.H. & Longsberg-Holm K.K. –1965– Rapid mixing and sampling techniques in biochemistry, Acad. Press, New York.
Douzou P. –1977– Cryobiochemistry: an introduction, Acad. Press, London.
Gutfreund H. –1965– An introduction to the study of enzymes, Blackwell Scientific Pub., Oxford.
Hammes G.G. –1982– Enzyme catalysis and regulation, Acad. Press, New York.
Le Maire M., Chabaud L. & Herve G. –1989– Un modèle d’étude: l’aspartate transcarbamylase. Théorie et guide d’expériences, Masson, Paris.
Roughton F.J.W. –1963– Rates and mechanisms of reactions, J. Wiley, New York.
General Reviews
Bergmeyer H.U. –1953– Methods of enzyme analysis, 10–13, Acad.Press, New York.
Eigen M. & Hammes G.G. –1963– Elementary steps in enzyme reactions (as studied by relaxation spectrometry), Adv. in Enzym. 25, 1–38.
Eigen M. & de Maeyer L. –1963– Relaxation methods, in Techniques in Organic chemistry, Vol. VIII, 2nd ed., Friess S.L., Lewis E.S. & Weissberger A. eds, 806–1054, Interscience, New York.
Roughton F.J.W. & Chance B. –1963– Rapid reactions, in Techniques in Organic chemistry, Vol. VIII, 2nd ed., Friess S.L., Lewis E.S. & Weissberger A. eds, 703–727, Interscience, New York.
Yon J. –1963– Etude des réactions enzymatiques, in Techniques de Laboratoire, Loiseleur éd., Tome II, 2e Partie, 936–959, Masson, Paris.
Fink A.L. –1977– Accounts for Chem. Res. 10, 233–239.
Fink A.L. & Geeves M.A. –1979– in Methods Enzymol. 63, 336–370.
Specialised Articles
Balny C., Saldana J.L. & Dahan N. –1984– Anal. Biochem. 139, 178–189.
Barwell C.J. & Hess B. –1970– Hoppe Seyler’s Physiol. Chem. 351, 1531–1536.
Bulone D., Capane A. & Cordone L. –1983– Biopolymers 22, 119–122.
Dreyfus M., Fries J. & Buc H. –1978– FEBS Lett. 95, 185–189.
Dreyfus M., Vendenbunder B., Tauc P. & Hervé G. –1984– Biochemistry 23, 4852–4859.
Easterby J.S. –1973– Biochim. Biophys. Acta 293, 552–558.
Fink A.L. –1973– Biochemistry 12, 1736.
Fink A.L. –1974– J. Biol. Chem. 249, 5027.
Fink A.L. –1976– Biochemistry 15, 1580.
Fink A.L. & Angelides K.J. –1975– Biochem. Biophys. Res. Commun. 64, 701.
Fink A.L., & Wildi –1974– J. Biol. Chem. 249, 6089.
Gekko K. & Timasheff S.N. –1981– Biochemistry 20, 4667-4686.
Goldman R. & Katchalski E. –1971– J. Theor. Biol. 32, 243–257.
Hart W.M. –1970– Mol. Pharmacol. 6, 31–40.
Heremans K., Juanwaert J. & Rijkenberg J. –1980– Rev. Sci. Instr. 51, 806–808.
Hervé G., Schmitt B. & Serre V. –2004– Cell. Mol. Biol. 4, 347–352.
Hui Bon Hoa G., Hamel G., Else A., Weill G. & Hervé G. –1990– Anal. Biochem. 187, 258–261.
Lee B. & Timasheff S.N. –1981– J. Biol. Chem. 256, 7193–7202.
Mc Lure W.R. –1969– Biochemistry 8, 2782–2786.
Phillips C.M., Mizutami Y. & Hochstrasser R.M. –1995– Proc. Natl Acad. Sci. USA 92, 7292–7296.
Regenfuss P., Clegg R.M., Fulwyler M.J., Barrantes F.J. & Jovin T.M. –1985– Rev. Sci. Instr. 56, 283–290.
Storer A.C. & Cornish-Bowden A. –1974– Biochem. J. 141, 205–209.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Yon-Kahn, J., Hervé, G. (2009). Experimental Methods to Study Enzymatic Reactions. In: Molecular and Cellular Enzymology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01228-0_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-01228-0_7
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-01227-3
Online ISBN: 978-3-642-01228-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)