Abstract
The existence of multifunctional enzymes and of multi-enzymatic complexes that catalyse consecutive reactions of a metabolic pathway is sometimes accompanied by important functional consequences for enzymatic activity regulation. The organisation of enzymes into multifunctional complexes can represent several advantages in vivo, for catalysis and its regulation. One of these consequences is the phenomenon of channelling, in which the product formed in a first reaction is channelled directly to the active site that catalyses the next reaction, thus avoiding the free diffusion of metabolites. This results in an increase in the global reaction rate.
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Bibliography
Specialised Articles
Ævarsson A., Chuang J.L., Wynn R.M., Turley S., Chuang D.T. & Hol W.H. –2000– Struct. Fold. Des. 8, 277.
Ævarsson A., Seger K., Turley S., Sokatch J.R. & Hol W.H. –1999– Nat. Struct. Biol. 6, 785.
Anderson K.S., Miles E.W. & Johnson K.A. –1991– J. Biol. Chem. 266, 8020.
Baldock C., Rafferty J.B., Struitze A.R., Cabas A.R. & Rice D.W. –1998– J. Mol. Biol. 284, 1529.
Chock P.B. & Gutfreund H. –1988– Proc. Natl Acad. Sci. USA 85, 8870.
Davidson J.N., Chen K.C., Jamison R.S., Musmanno L.A. & Kern C.B. –1993– The evolutionary history of the first three enzymes in pyrimidine biosynthesis, in Bioessays 15, 157.
Elcock A.H. & Mccammon A. –1996– Biochemistry 35, 12652.
Hervé G., Nagy M., Le Gouar M., Penverne B. & Ladjimi M. –1993– Biochem. Soc. Trans. 21, 195.
Holden H.M., Thoden J.B. & Raushel F.M. –1998– Carbamyl phosphate synthetase, in Curr. Op. Struct. Biol. 8, 679.
Horowitz N.H. –1945– Proc. Natl. Acad. Sci. USA 31, 153.
Hur O., Leja C. & Dunn M. –1996– Biochemistry 35, 7378.
Hyde C.C., Ahmed A., Padlan E.A., Miles E.W. & Davies D.R. –1988– J. Biol. Chem. 263, 17857.
Izard T., Ævarsson A., Allen M.D., Westphal A.H., Perham R.N., De Kok A. & Hol W.G.J. –1999– Proc. Natl Acad. Sci. USA 96, 1240.
Keleti T., Batke J., Ovadi J., Jancsik V. & Bartha F. –1977– Adv. Enzyme Regul. 15, 233.
Kimoto T., Nishigai M., Sasaki T. & Ikai A. –1988– J. Mol. Biol. 203, 183.
Kirschner K., Szadkowski H., Jardetzky T.S. & Hager V. –1987– Methods Enzymol. 142, 386–397.
Kleiger G., Perry J. & Eisenberg D. –2001– Biochemistry 40, 14484.
Krahn J.M., Kim J.H., Burns M.R., Parry J., Zalkin H. & Smith J.L. –1997– Biochemistry 36, 11061.
Lehotsky J., Befakova G., Kaplan P. & Raeymaekers L. –1993– Gen. Physiol. Biophys. 12, 339.
Lindbladh C., Rault M., Hagglund C., Small W.C., Mosbach K., Bülow L., Evans C. & Srere P.A. –1994– Biochemistry 33, 11692.
Linn T.C. & Srere P.A. –1980– J. Biol. Chem. 255, 10676.
Luger K., Hommel U., Herold M., Hofsteenge J. & Kirschner K. –1989– Science 243, 206.
Macgregor J.S., Singh V.N., Davoust S., Melloni E., Pontremoli S. & Horecker B.L. –1980– Proc. Natl. Acad. Sci. USA 77, 3889.
Mattevi A., Obmolova G., Schulze E., Kalk K.H., Westphal A.H., De Kok A. & Hol W.G.J. –1992– Science 255, 1544.
Ovadi J. –1988– TIBS 13, 486.
Ovadi J., Salerno C., Keleti T. & Fasella P. –1978– Eur. J. Biochem. 90, 499.
Penverne B., Belkaïd M. & Hervé G. –1994– Arch. Biochem. Biophys. 309, 85.
Perham R.N. –1991– Biochemistry 30, 8501.
Priestle J.P., Grütter M.G., White J.L., Vincent M.G., Kania M., Wilson E., Jardetzky T.S., Kischner K. & Jansonius J.N. –1987– Proc. Natl Acad. Sci. USA 84, 5680.
Reed L.J. –1974– Accounts Chem. Res. 7, 40.
Schlichting I., Yang X.J., Miles E.W., Kim A.Y. & Anderson K.S. –1994– J. Biol. Chem. 269, 26591.
Scylly J.L. & Evans D.R. –1991– Proteins: Struct. Funct. Genet. 9, 191.
Serre L., Verbree E.C., Dauter Z., Stuitje A.R. & Derewenda Z.S. –1995– J. Biol. Chem. 270, 12961.
Smith S., Stern A., Randhawa Z.I. & Knudsn J. –1985– Eur. J. Biochem. 152, 547.
Souciet J.L., Nagy M., Le Gouar M., Lacroute F. & Potier S. –1989– Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex, in Gene 79, 59.
Srivastava D.K. & Bernhard S.A. –1986a– Curr. Top. Cell. Regul. 28, 1.
Srivastava D.K. & Bernhard S.A. –1986b– Science 234, 1081.
Srivastava D.K., Smolen P., Betts G.F., Fukushima T., Spivey H.O. & Bernhard S.A. –1989– Proc. Natl Acad. Sci. USA 86, 6464.
Thoden J.B., Holden H.M., Wesenberg G., Rauschel F.M. & Rayment I. –1997– Biochemistry 36, 6305.
Trujillo M., Donald R.G., Roos D.S., Green P.J. & Santi D.V. –1996– Biochemistry 35, 6366.
wakil S.J., Stoops J.K. & Joshi V.C. –1983– Annu. Rev. Biochem. 52, 537.
Wallis N.G., Allen M.D., Broadhurst R.W., Lessard I.A.D. & Perham R.N. –1996– J. Mol. Biol. 263, 464.
Walsh G.R. & Easterby J.S. –1994– Trends Biochem. Sci. 19, 193.
Weber J.P. & Bernhard S.A. –1982– Biochemistry 21, 4189.
Wieland F., Siess E.A., Renner L., Verfürth C. & Lynen F. –1978– Proc. Natl Acad. Sci. USA 75, 5792.
Wilmanns M., Priestle J.P., Niermann T. & Jansonius J.N. –1992– J. Mol. Biol. 223, 477.
Wilmanns M., Hyde C.C., Davies D.R., Kirschner K. & Jansonius J.N. –1991– Biochemistry 30, 9161.
Wu X., Gutfreund H., Lakatos S. & Cock P.B. –1991– Proc. Natl Acad. Sci. USA 88, 497.
Yoshida S., Tamiya-korzymi K. & Kojima K. –1989– Biochim. Biophys Acta 1007, 61
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Yon-Kahn, J., Hervé, G. (2009). Multifunctional Enzymes, Multi-Enzymatic Complexes and Metabolic Channelling. In: Molecular and Cellular Enzymology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01228-0_16
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DOI: https://doi.org/10.1007/978-3-642-01228-0_16
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