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Humanized Mice pp 179–196Cite as

In Vivo Imaging in Humanized Mice

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 324))

The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Keio University School of Medicine, 160-8582, Tokyo, Japan

    H. Masuda, H. J. Okano, T. Maruyama, Y. Yoshimura, H. Okano & Y. Matsuzaki

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  1. H. Masuda
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  2. H. J. Okano
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  3. T. Maruyama
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  4. Y. Yoshimura
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  5. H. Okano
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  6. Y. Matsuzaki
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Editors and Affiliations

  1. Central Institute for Experimental Animals, 216-0001, Kawasaki, Japan

    Tatsuji Nomura M.D, Ph.D

  2. RIKEN, Yokohama, Japan

    Takeshi Watanabe M.D, Ph.D

  3. Tokai University School of Medicine, Kanagawa, Japan

    Sonoko Habu M.D, Ph.D

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© 2008 Springer-Verlag Berlin Heidelberg

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Masuda, H., Okano, H.J., Maruyama, T., Yoshimura, Y., Okano, H., Matsuzaki, Y. (2008). In Vivo Imaging in Humanized Mice. In: Nomura, T., Watanabe, T., Habu, S. (eds) Humanized Mice. Current Topics in Microbiology and Immunology, vol 324. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-75647-7_12

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