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Practical Bioinformatics pp 139–168Cite as

Bioinformatics-Guided Identification and Experimental Characterization of Novel RNA Methyltransferas

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Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 15))

Naturally occurring RNAs contain numerous chemically altered nucleosides. They are formed by enzymatic modification of the primary transcripts during the complex RNA maturation process. To date, a total of 96 structurally distinguishable modified nucleosides originating from different types of RNAs from many diverse organisms of the three major phylogenetic domains of life have been reported (Rozenski et al. 1999); http://medstat.med.utah.edu/RNAmods; and references therein). The pattern of modifications (type and location) depends on the RNA molecule considered, as well as, on the organism or the organelle they originate from.However, the largest number of modified nucleosides with the greatest structural diversity (a total of 81) is found in transfer RNAs, especially in tRNAs from higher organisms (Sprinzl et al. 1998; http://www.uni-bayreuth.de/departments/biochemie/trna). Other types of RNA (snRNA, snoRNA, rRNA,mRNA) also contain modified nucleosides (see http://rna.wustl.edu/snoRNAdb), however, their occurrence and particularly their diversity are lower than in tRNAs (see, for example,Limbach et al. 1995;Motorin and Grosjean 1998).

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Authors and Affiliations

  1. Bioinformatics Laboratory, International Institute of Molecular and Cell Biology in Warsaw, Trojdena 4, 02-109, Warsaw, Poland

    Dr. Janusz M. Bujnicki

  2. Laboratoire de Microbiologie, Université Libre de Bruxelles, 1 av. E.Gryson, 1070, Bruxelles, Belgium

    Louis Droogmans

  3. Laboratory of Structural Enzymology and Biochemistry, CNRS, 1 av. De la Terrasse, 91198, Gif-sur-Yvette, France

    Henri Grosjean

  4. Centre de Recherche de Biochimie Macromoléculaire du CNRS, 1919 Route de Mende, 34293, Montpellier Cedex, France

    Suresh K. Purushothaman & Bruno Lapeyre

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  1. Dr. Janusz M. Bujnicki
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  2. Louis Droogmans
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  3. Henri Grosjean
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  4. Suresh K. Purushothaman
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  5. Bruno Lapeyre
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  1. International Institute of Molecular and Cell Biology in Warsaw, Trojdena 4, 02-109, Warsaw, Poland

    Janusz M. Bujnicki

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Bujnicki, J.M., Droogmans, L., Grosjean, H., Purushothaman, S.K., Lapeyre, B. (2008). Bioinformatics-Guided Identification and Experimental Characterization of Novel RNA Methyltransferas. In: Bujnicki, J.M. (eds) Practical Bioinformatics. Nucleic Acids and Molecular Biology, vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74268-5_7

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  • DOI: https://doi.org/10.1007/978-3-540-74268-5_7

  • Publisher Name: Springer, Berlin, Heidelberg

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