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Paradigmatic Role of Galactose Switch

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Galactose Regulon of Yeast

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In classical genetics, inferences are based on the phenotypic differences between a wild-type and a mutant organism. This old-fashioned systems biology continues to provide deep insights into the genetic basis of fundamental biological processes mainly at the phenomenological level. This limitation was partly overcome by the revolutionary developments in recombinant DNA technology (Chap. 5). With this, it was possible to isolate, manipulate in vitro, and reintroduce a gene into the organism to determine and evaluate its effects on the performance at the phenotypic level. The impact of these combined approaches in deciphering the mechanistic details of molecular interactions has been impressive. However, biological problems are too challenging to be understood, even with these powerful approaches. Nevertheless, the classical and molecular genetic approaches culminated in a large body of data including the whole genome sequence of different organisms resulting in a paradigm shift in experimental biology beyond expectations.

In no other experimental organism genomic approach has been so successfully used than in S. cerevisiae. This is because yeast has been the vanguard of biochemical, genetic, and cell biological studies. An existing wealth of knowledge of yeast biology was put to great advantage in the context of sequence information. One of the immediate outcomes of the genome sequence is “expression profiling” using micro-array analysis. This technique has become quite routine and has yielded valuable information not only in yeast but also in almost all organisms whose genome sequence is known.

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(2008). Paradigmatic Role of Galactose Switch. In: Galactose Regulon of Yeast. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74015-5_8

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