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Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes

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Protein Engineering

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 22))

Native chemical ligation (NCL) is a chemoselective reaction that joins synthetic or recombinant peptide and protein fragments through a native peptide bond. Advances in generating the required peptide and protein components for NCL have enabled the homogeneous incorporation of unnatural amino acids, fluorescent probes, and other modifications into protein domains and full-length proteins. The resulting semisynthetic proteins, which can be made in multi-milligram quantities, may be used in biophysical and biochemical studies to address challenging biological problems not accessible with traditional methods. This chapter provides an overview of NCL, including relevant design considerations and technological advances, and a discussion of recent applications that apply protein-based probes accessed through NCL.

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Authors and Affiliations

  1. Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA

    E. Vogel Taylor & B. Imperiali

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  1. E. Vogel Taylor
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  2. B. Imperiali
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  1. Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA

    Caroline Köhrer  & Uttam L. RajBhandary  & 

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Vogel Taylor, E., Imperiali, B. (2009). Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes. In: Köhrer, C., RajBhandary, U.L. (eds) Protein Engineering. Nucleic Acids and Molecular Biology, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70941-1_3

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