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cGMP: Generators, Effectors and Therapeutic Implications pp 229–243Cite as

Novel Techniques for Real-Time Monitoring of cGMP in Living Cells

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Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 191))

Abstract

Recent developments of biophysical and electrophysiological techniques have enabled researchers to monitor levels of free intracellular cGMP in real-time and in intact living cells. These techniques are based on the use of cGMP sensors, which respond to cGMP with changes in transmembrane ion current or changes in fluorescence. Here, we describe the principles of these techniques, compare them in terms of sensitivity and discuss possible application for current cell biology and physiology.

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Author information

Authors and Affiliations

  1. Institute of Pharmacology and Toxicology, University of Würzburg, Germany

    Viacheslav O. Nikolaev

  2. Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Würzburg, Versbacher Strasse 9, Würzburg, 97078, Germany

    Martin J. Lohse

Authors
  1. Viacheslav O. Nikolaev
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  2. Martin J. Lohse
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Editor information

Editors and Affiliations

  1. Dept. Pharmacology, Monash University, Wellington Road, Clayton, VIC, 3800, Australia

    Harald H. H. W. Schmidt

  2. Inst. Pharmakologie und Toxikologie, TU München, Biedersteiner Str. 29, München, 80802, Germany

    Franz Hofmann

  3. Bayer Schering Pharma AG Cardiology Research, Pharma Research Center, Wuppertal, 42096, Germany

    Johannes-Peter Stasch

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Nikolaev, V.O., Lohse, M.J. (2009). Novel Techniques for Real-Time Monitoring of cGMP in Living Cells. In: Schmidt, H.H.H.W., Hofmann, F., Stasch, JP. (eds) cGMP: Generators, Effectors and Therapeutic Implications. Handbook of Experimental Pharmacology, vol 191. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-68964-5_11

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