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Applied Scanning Probe Methods VI pp 165–203Cite as

Direct Detection of Ligand-Protein Interaction Using AFM

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Part of the book series: NanoScience and Technology ((NANO))

16.5 Summary

A natural question arises why the receptor-ligand binding is so important in biological systems and why it is so deeply investigated. The answer is the following. First, the binding between molecules is one of the methods that cells utilize to interact with a huge variety of different types of molecules that are delivered not only from the outside environment but also with those that are generated within the cell itself. Secondly, the binding is the basis of a great number of normal biological functions such as immunological response, embryogenesis, cell growth, differentiation, gene regulation, enzyme catalysis, etc., that are crucial for our life. The loss or alterations of these interactions can affect cell functioning and may lead to many pathological states. Numerous diseases are related to malfunctioning of these molecular recognition processes. Investigations of the interaction mechanism are crucial for understanding a wide spectrum of biological processes present during inflammation or cancer metastasis, which should lead, among other applications, to the development of highly specific drugs. The biological cell adhesion is a complex process and, in most cases, it is not possible to write down a set of universal laws that explain the cell-adhesion phenomena. Such techniques as atomic force microscopy deliver only the possibility of obtaining the detailed and quantitative characterization of various types of involved interactions.

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Author information

Authors and Affiliations

  1. The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342, Kraków, Poland

    Małgorzata Lekka

  2. Institute of Medical Biochemistry, Collegium Medicum Jagiellonian University, Kopernika 7, 31-034, Kraków, Poland

    Piotr Laidler

  3. Ecole Polytechnique Fédérale de Lausanne, EPFL - IPMC - NN, 1015, Lausanne, Switzerland

    Andrzej J. Kulik

Authors
  1. Małgorzata Lekka
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  2. Piotr Laidler
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  3. Andrzej J. Kulik
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Editor information

Editors and Affiliations

  1. Nanotribology Laboratory for Information Storage and MEMS/NEMS (NLIM) 390 Scott Laboratory, The Ohio State University, 201 W. 19th Avenue, Columbus, Ohio, 43210-1142, USA

    Bharat Bhushan

  2. Graduate School of Science, Department of Mathematics, Osaka City University, Sugimoto 3-3-138, 558-8585, Osaka, Japan

    Satoshi Kawata

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Lekka, M., Laidler, P., Kulik, A.J. (2007). Direct Detection of Ligand-Protein Interaction Using AFM. In: Bhushan, B., Kawata, S. (eds) Applied Scanning Probe Methods VI. NanoScience and Technology. Springer, Berlin, Heidelberg . https://doi.org/10.1007/978-3-540-37319-3_6

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