Skip to main content
SpringerLink
Log in

Single-Molecule Studies on Cells and Membranes Using the Atomic Force Microscope

  • Chapter
Book cover Applied Scanning Probe Methods VI

Part of the book series: NanoScience and Technology ((NANO))

14.1 Abstract

Atomic force microscopy (AFM) has been developed into an imaging method that yields fine structural details on biological samples in their physiological environment. Topographic AFM imaging enables a broad range of biomolecular complexes that are bound to the cells and membranes to be investigated. Dynamic processes can be visualized, single biomolecules can be observed at work, and the formation of biomolecular assemblies can be studied. In addition to high-resolution imaging, the measurement of mechanical forces at the molecular level has provided detailed insights into structure-function relationships of many biological systems. The detection of inter- and intramolecular forces yields information of structural parameters of the binding pocket, on the molecular dynamics of the recognition process, and on the energy landscape of the interaction. Single-molecule recognition measurements have also been demonstrated on cells and membranes and open new perspectives in exploring the regulation of cellular processes. By combining topographic imaging with force measurements, receptor binding sites can be localized with nanometer accuracy rendering possible the identification of specific components on the cellular surfaces.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Binnig G, Quate CF, Gerber C (1986) Phys Rev Lett 56:930

    Article  Google Scholar 

  2. Horber JK, Miles MJ (2003) Science 302:1002

    Article  CAS  Google Scholar 

  3. Mueller DJ, Janovjak H, Lehto T, Kuerschner L, Anderson K (2002) Prog Biophys Mol Biol 79:1

    Article  CAS  Google Scholar 

  4. Scheuring S, Levy D, Rigaud JL (2005) Biochim Biophys Acta 1712:109

    Article  CAS  Google Scholar 

  5. Malkin A, Plomp J, McPherson A (2005) Meth Mol Biol 292:85

    CAS  Google Scholar 

  6. Jeremic A, Kelly M, Cho SJ, Stromer MH, Jena BP (2003) Biophys J 85:2035

    CAS  Google Scholar 

  7. Franz CM, Muller DJ (2005) J Cell Sci 118:5315

    Article  CAS  Google Scholar 

  8. Zaman MS, Goyal A, Dubey GP, Gupta PK, Chandra SL, Das TK, Ganguli M, Singh Y (2005) Microsc Res Tech 66:307

    Article  Google Scholar 

  9. Engel A, Muller DJ (2000) Nat Struct Biol 7:715

    Article  CAS  Google Scholar 

  10. Allison DP, Hinterdorfer P, Han W (2002) Curr Opin Biotechnol 13:47

    Article  CAS  Google Scholar 

  11. Rief M, Oesterhelt F, Heymann B, Gaub HE (1997) Science 275:1295

    Article  CAS  Google Scholar 

  12. Marzsalek PE, Oberhauser AF, Pang YP, Fernandez JM (1998) Nature 396:661

    Article  CAS  Google Scholar 

  13. Smith S, Cui Y, Bustamante C (1996) Science 271:795

    Article  CAS  Google Scholar 

  14. Strick TR, Allemend JF, Bensimon D, Bensimon A, Croquette V (1996) Biophys J 271:1835

    CAS  Google Scholar 

  15. Veigel C, Coluccio LM, Jontes JD, Sparrow JC, Milligan RA, Molloy JE (1999) Nature 398:530

    Article  CAS  Google Scholar 

  16. Tanaka H, Homma K, Iwane AH, Katayama E, Ikebe R, Saito T, Yanagida T, Ikebe M (2002) Nature 415:129

    Article  Google Scholar 

  17. Lehenkari PP, Horton MA (1999) Biochem Biophys Res Com 259:645

    Article  CAS  Google Scholar 

  18. Benoit M, Gabriel D, Gerisch G, Gaub HE (2000) Nat Cell Biol 2:313

    Article  CAS  Google Scholar 

  19. Evans E (2001) Annu Rev Biophys Biomol Struct 30:105

    Article  CAS  Google Scholar 

  20. Kienberger F, Ebner A, Gruber HJ, Hinterdorfer P (2006) Acc Chem Res 39:29

    Article  CAS  Google Scholar 

  21. Sarid D (1991) Scanning Force Microscopy, Oxford University Press, New York

    Google Scholar 

  22. Putman CAJ, Vanderwerf KO, de Grooth BG, Vanhulst NF, Greve J (1994) Appl Phys Lett 64:2454

    Article  CAS  Google Scholar 

  23. Hansma PK, Cleveland JP, Radmacher M, Walters DA, Hillner PE, Bezanilla M, Fritz M, Vie D, Hansma HG, Prater CB, Massie J, Fukunaga L, Gurley J, Elings V (1994) Appl Phys Lett 64:1738

    Article  CAS  Google Scholar 

  24. Han W, Lindsay SM, Jing T (1996) Appl Phys Lett 69:1

    Article  Google Scholar 

  25. Kienberger F, Stroh C, Kada G, Moser R, Baumgartner W, Pastushenko V, Rankl C, Schmidt U, Muller H, Orlova E, LeGrimellec C, Drenckhahn D, Blaas D, Hinterdorfer P (2003) Ultramicroscopy 97:229

    Article  CAS  Google Scholar 

  26. Muller DJ, Schabert FA, Buldt G, Engel A (1995) Biophys J 68:1681

    CAS  Google Scholar 

  27. Kienberger F, Zhu R, Moser R, Blaas D, Hinterdorfer P (2004) J Virol 78:3203

    Article  CAS  Google Scholar 

  28. Lantz M, Liu YZ, Cui XD, Tokumoto H, Lindsay SM (1999) Interface Anal 27:354

    Article  CAS  Google Scholar 

  29. Moeller C, Allen M, Elings V, Engel A, Muller DJ (1999) Biophys J 77:1050

    Google Scholar 

  30. Muller DJ, Amrein M, Engel A (1997) J Struct Biol 119:172

    Article  CAS  Google Scholar 

  31. Scheuring S, Sturgis JN (2005) Science 309:484

    Article  CAS  Google Scholar 

  32. Ando T, Kodera N, Takai E, Maruyama D, Saito K, Toda A (2001) Proc Natl Acad Sci USA 98:12468

    Article  CAS  Google Scholar 

  33. Scheuring S, Reiss-Husson F, Engel A, Rigaud JL, Ranck JL (2001) The EMBO Journal 20:3029–3035

    Article  CAS  Google Scholar 

  34. Pum D, Neubauer A, Gyoervary E, Sara M, Sleytr UB (2000) Nanotechnol 11:100

    Article  CAS  Google Scholar 

  35. Sara M, Sleytr UB (2000) J Bacteriol 182:859

    Article  CAS  Google Scholar 

  36. Sara M, Pum D, Schuster B, Sleytr UB (2005) J Nanosci Nanotechnol 5:1939

    Article  CAS  Google Scholar 

  37. Ebner A, Kienberger F, Huber C, Kamruzzahan ASM, Pastushenko V, Tang J, Kada G, Gruber HJ, Sleytr UB, Sara M, Hinterdorfer P (2006) Chem Bio Chem 7:588

    CAS  Google Scholar 

  38. Pante N, Aebi U (1993) J Cell Biol 122:977

    Article  CAS  Google Scholar 

  39. Shahin V, Albermann L, Schillers H, Kastrup L, Schaefer C, Ludwig Y, Stock C, Oberleithner H (2005) J Cell Phys 202:591

    Article  CAS  Google Scholar 

  40. Schaefer C, Shahin V, Albermann L, Hug MJ, Reinhardt J, Schillers H, Schneider SW, Oberleithner H (2002) Proc Natl Acad Sci USA 99:7154

    Article  CAS  Google Scholar 

  41. Chiu W, Baker ML, Jiang W, Dougherty M, Schmid MF (2005) Structure 13:363

    Article  CAS  Google Scholar 

  42. Kuznetsov YG, Malkin AJ, Lucas RW, Plomp M, McPherson A (2001) J Gen Virol 82:2025

    CAS  Google Scholar 

  43. Kienberger F, Rankl C, Pastushenko V, Zhu R, Blaas D, Hinterdorfer P (2005) Structure 13:1247

    Article  CAS  Google Scholar 

  44. Kuznetsov YG, Victoria JG, Robinson W, McPherson A (2003) J. Virol. 77:11896

    Article  CAS  Google Scholar 

  45. Svoboda K, Schmidt CF, Schnapp BJ, Block SM (1993) Nature 365:721

    Article  CAS  Google Scholar 

  46. Evans E, Ritchie K, Merkel R (1995) Biophys J 68:2580

    CAS  Google Scholar 

  47. Clausen-Schaumann H, Seitz M, Krautbauer R, Gaub HE (2000) Curr Opin Chem Biol 4:524

    Article  CAS  Google Scholar 

  48. Hinterdorfer P, Baumgartner W, Gruber HJ, Schilcher K, Schindler H (1996) Proc Natl Acad Sci USA 93:3477

    Article  CAS  Google Scholar 

  49. Hinterdorfer P (2002) Meth Cell Biol 68:115

    Article  CAS  Google Scholar 

  50. Hinterdorfer P, Gruber HJ, Kienberger F, Kada G, Riener C, Borken C, Schindler H (2002) Coll Surf B 23:115

    Article  CAS  Google Scholar 

  51. Grandbois M, Dettmann W, Benoit M, Gaub HE (1999) Science 283:1727

    Article  CAS  Google Scholar 

  52. Hinterdorfer P, Schilcher K, Baumgartner W, Gruber HJ, Schindler H (1998) Nanobiology 4:177

    CAS  Google Scholar 

  53. Lyubchenko YL, Gall AA, Shlyakhtenko LS (2001) Meth Mol Biol 148:569

    CAS  Google Scholar 

  54. Riener CK, Stroh CM, Ebner A, Klampfl C, Gall AA, Romanin C, Lyubchenko YL, Hinterdorfer P, Gruber HJ (2003) Anal Chim Acta 479:59

    Article  CAS  Google Scholar 

  55. Hinterdorfer P, Kienberger F, Raab A, Gruber HJ, Baumgartner W, Kada G, Riener C, Wielert-Badt S, Borken C, Schindler H (2000) Single Mol 1:99

    Article  CAS  Google Scholar 

  56. Riener CK, Kienberger F, Hahn CD, Buchinger GM, Egwim IOC, Haselgruebler T, Ebner A, Romanin C, Klampfl C, Lackner B, Prinz H, Blaas D, Hinterdorfer P, Gruber HJ (2003) Anal Chim Acta 497:101

    Article  CAS  Google Scholar 

  57. Kienberger F, Pastushenko V, Kada G, Gruber HJ, Riener C, Schindler H, Hinterdorfer P (2000) Single Mol 1:123

    Article  CAS  Google Scholar 

  58. Pfister G, Stroh CM, Perschinka H, Kind M, Knoflach M, Hinterdorfer P, Wick G (2005) J Cell Sci 118:1587

    Article  CAS  Google Scholar 

  59. Bell GI (1978) Science 200:618

    Article  CAS  Google Scholar 

  60. Evans E, Ritchie K (1997) Biophys J 72:1541

    Article  CAS  Google Scholar 

  61. Evans E, Ritchie K (1999) Biophys J 76:2439

    CAS  Google Scholar 

  62. Strunz T, Oroszlan K, Schumakovitch I, Guntherodt H, Hegner M (2000) Biophys J 79:1206

    CAS  Google Scholar 

  63. Merkel R, Nassoy P, Leung A, Ritchie K, Evans E (1999) Nature 397:50

    Article  CAS  Google Scholar 

  64. Chen A, Moy VT (2000) Biophys J 78:2814

    CAS  Google Scholar 

  65. Zhang X, Wojcikiewicz E, Moy VT (2002) Biophys J 83:2270

    CAS  Google Scholar 

  66. Puntheeranurak T, Wildling L, Gruber HJ, Kinne KH, Hinterdorfer P (2006) submitted

    Google Scholar 

  67. Xia X, Lin JT, Kinne RK (2003) Biochemistry 42:6115

    Article  CAS  Google Scholar 

  68. Raja MM, Tyagi NK, Kinne RK (2003) J Biol Chem 278:49154

    Article  CAS  Google Scholar 

  69. Carrion-Vazquez M, Oberhauser AF, Fowler SB, Marszalek PE, Broedel SE, Clarke J, Fernandez JM (1999) Proc Natl Acad Sci USA 96:3694

    Article  CAS  Google Scholar 

  70. Rief M, Gautel M, Oesterhelt F, Fernandez JM, Gaub HE (1997) Science 276:1109

    Article  CAS  Google Scholar 

  71. Marszalek PE, Pang YP, Li H, El Yazal J, Oberhauser AF, Fernandez JM (1999) Proc Natl Acad Sci USA 96:7894

    Article  CAS  Google Scholar 

  72. Li H, Rief M, Oesterhelt F, Gaub HE (1998) Adv Mater 3:316

    Article  Google Scholar 

  73. Rief M, Clausen-Schaumann H, Gaub HE (1999) Nat Struct Biol 6:346

    Article  CAS  Google Scholar 

  74. Hertadi R, Gruswitz F, Silver L, Koide A, Koide S, Arakawe H, Ikai A (2003) J Mol Biol 333:993

    Article  CAS  Google Scholar 

  75. Kedrov A, Ziegler C, Janovjak H, Kuehlbrandt W, Muller DJ (2004) J Mol Biol 340:1143

    Article  CAS  Google Scholar 

  76. Kedrov A, Janovjak H, Ziegler C, Kuehlbrandt W, Muller DJ (2006) J Mol Biol 355:2

    Article  CAS  Google Scholar 

  77. Willemsen OH, Snel MME, van der Werf KO, de Grooth BG, Greve J, Hinterdorfer P, Gruber HJ, Schindler H, van Kyook Y, Figdor CG (1998) Biophys J 57:2220

    Google Scholar 

  78. Raab A, Han W, Badt D, Smith-Gill SJ, Lindsay SM, Schindler H, Hinterdorfer P (1999) Nat Biotech 17:1999

    Google Scholar 

  79. Stroh CM, Ebner A, Geretschlaeger M, Freudenthaler G, Kienberger F, Kamruzzahan ASM, Smith-Gill SJ, Gruber HJ, Hinterdorfer P (2004) Biophys J 87:1981

    Article  CAS  Google Scholar 

  80. Stroh CM, Wang H, Bash R, Ashcroft B, Nelson J, Gruber H, Lohr D, Lindsay SM, Hinterdorfer P (2004) Proc Natl Acad Sci USA 101:12503

    Article  CAS  Google Scholar 

  81. Ebner A, Kienberger F, Kada G, Stroh CM, Geretschlager M, Kamruzzahan ASM, Wildling L, Johnson WT, Ashcroft B, Nelson J, Lindsay SM, Gruber HJ, Hinterdorfer P (2005) Chem Phys Chem 6:897

    CAS  Google Scholar 

  82. Chtcheglova LA, Waschke J, Kada G, Drenckhahn D, Hinterdorfer P (2006) (manuscript in preparation)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Biophysics, Johannes Kepler University of Linz, Altenbergerstr. 69, A-4040, Linz, Austria

    Ferry Kienberger, Lilia A. Chtcheglova, Andreas Ebner, Theeraporn Puntheeranurak, Hermann J. Gruber & Peter Hinterdorfer

Authors
  1. Ferry Kienberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lilia A. Chtcheglova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andreas Ebner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Theeraporn Puntheeranurak
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hermann J. Gruber
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Peter Hinterdorfer
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Nanotribology Laboratory for Information Storage and MEMS/NEMS (NLIM) 390 Scott Laboratory, The Ohio State University, 201 W. 19th Avenue, Columbus, Ohio, 43210-1142, USA

    Bharat Bhushan

  2. Graduate School of Science, Department of Mathematics, Osaka City University, Sugimoto 3-3-138, 558-8585, Osaka, Japan

    Satoshi Kawata

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Kienberger, F., Chtcheglova, L.A., Ebner, A., Puntheeranurak, T., Gruber, H.J., Hinterdorfer, P. (2007). Single-Molecule Studies on Cells and Membranes Using the Atomic Force Microscope. In: Bhushan, B., Kawata, S. (eds) Applied Scanning Probe Methods VI. NanoScience and Technology. Springer, Berlin, Heidelberg . https://doi.org/10.1007/978-3-540-37319-3_4

Download citation

Publish with us

Policies and ethics

Search

Navigation