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Conditional Mutagenesis: An Approach to Disease Models pp 235–262Cite as

Analysis of Mouse Development with Conditional Mutagenesis

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Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 178))

Abstract

Explorations into the molecular embryology of the mouse have played a vital role in our understanding of the basic mechanisms of gene regulation that govern development and disease. In the last 15 years, these mechanisms have been analyzed with vastly greater precision and clarity with the advent of systems that allow the conditional control of gene expression. Typically, this control is achieved by silencing or activating the gene of interest with site-specific DNA recombination or transcriptional transactivation. In this review, I discuss the application of these technologies to mouse development, focusing on recent innovations and experimental designs that specifically aid the study of the mouse embryo.

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  1. Laboratory of Cancer and Developmental Biology, NCI-Frederick, National Institutes of Health, Frederick, MD, 21702-1201, USA

    M. Lewandoski

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  1. Interfakultäres Institut für Biochemie (IFIB) Signaltransduktion — transgene Modelle, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Robert Feil

  2. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1, rue Laurent Fries, 67404, Illkirch, France

    Daniel Metzger

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Lewandoski, M. (2007). Analysis of Mouse Development with Conditional Mutagenesis. In: Feil, R., Metzger, D. (eds) Conditional Mutagenesis: An Approach to Disease Models. Handbook of Experimental Pharmacology, vol 178. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35109-2_10

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