Chapter

Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

pp 197-221

Date:

Genome-Wide Analysis of DNA Methylation Patterns by High-Throughput Sequencing

  • Tuncay BaubecAffiliated withEpigenomics and Chromatin Biology Lab, Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich
  • , Altuna AkalinAffiliated withBioinformatics Platform, Berlin Institute for Medical Systems Biology, Max Delbrück Centre Email author 

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Abstract

Epigenetic modifications of chromatin and DNA are relevant for eukaryotic gene expression. DNA methylation is the paradigm epigenetic modification that is associated with transcriptional repression. Perturbations of DNA methylation patterns are frequently associated with cancer and aging, raising a great interest in understanding the contribution of this mark to human health. High-throughput sequencing allows interrogating the status of methylated DNA at nucleotide resolution and genome-wide, bringing unprecedented views on the distribution and dynamics of this relevant modification in healthy and diseased tissues. Here we discuss commonly used wet-lab methodologies and computational approaches to identify DNA methylation patterns and measure their dynamics during biological processes in a quantitative and unbiased manner.

Keywords

Epigenetic modifications DNA methylation