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Microfluidic Methods for Molecular Biology pp 235–252Cite as

Wire-guided Droplet Manipulation for Molecular Biology

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Abstract

Wire-guided droplet manipulation (WDM) is a simple method of manipulating liquid droplets in a hydrophobic environment to conduct experiments, reactions, and assays. In WDM, a wire (or needle tip) manipulates microliter-sized liquid droplets within an immiscible liquid or on a hydrophobic surface. The attributes of this format for liquid handling address some of the challenges facing the use of conventional techniques. Specifically, WDM provides solutions for development of automated, sample-to-answer, point-of-care systems with potential applications in medicine, life science research, forensics, veterinary diagnostics, and disease control.

The widespread applicability of this technique is due to its inherent simplicity stemming from the attractive force between the droplet and the wire. The physics of this interaction will be explained in this chapter. WDM can be applied to standard protocols and is easily reprogrammable for different liquid handling applications. Dilution, mixing, centrifugation, and thermocycling have all been automated by WDM (You and Yoon, J Biol Eng 6:15, 2012). If desired, the principles of droplet manipulation can be easily integrated into the common scientific automation strategy, using commercially available robotic pipetting systems. WDM is automatable, reprogrammable, easy to use, and robust. These are essential features of rapid, all-in-one, sample-to-answer systems to be used at the point-of-care.

The applications of WDM within molecular biology that have been demonstrated include DNA extraction (lysing, precipitation, washing, and rehydration), nanoparticle surface deposition for fabrication of a protein nanoarray, immunoassay, PCR thermocycling, and real-time PCR.

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References

  1. You DJ, Yoon J-Y (2012) Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations. J Biol Eng 6:15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Schneider T, Kreutz J, Chiu DT (2013) The potential impact of droplet microfluidics in biology. Anal Chem 85:3476–3482

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zec H, Shin DJ, Wang T-H (2014) Novel droplet platforms for the detection of disease biomarkers. Expert Rev Mol Diagn 14:787–801

    Article  CAS  PubMed  Google Scholar 

  4. Cho SK, Moon H, Kim C-J (2003) Creating, transporting, cutting, and merging liquid droplets by electrowetting-based actuation for digital microfluidic circuits. J Microelectromech Syst 12:70–80

    Article  Google Scholar 

  5. Barbulovic-Nad I, Au SH, Wheeler AR (2010) A microfluidic platform for complete mammalian cell culture. Lab Chip 10:1536–1542

    Article  CAS  PubMed  Google Scholar 

  6. Egatz-Gómez A, Melle S, García AA, Lindsay SA, Márquez M, Domínguez-García P, Rubio MA, Picraux ST, Taraci JL, Clement T, Yang D, Hayes MA, Gust D (2006) Discrete magnetic microfluidics. Appl Phys Lett 89:034106

    Article  Google Scholar 

  7. Ohashi T, Kuyama H, Hanafusa N, Togawa Y (2007) A simple device using magnetic transportation for droplet-based PCR. Biomed Microdevices 9:695–702

    Article  CAS  PubMed  Google Scholar 

  8. Zhang Y, Wang T-H (2013) Full-range magnetic manipulation of droplets via surface energy traps enables complex bioassays. Adv Mater 25:2903–2908

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhang Y, Shin DJ, Wang T-H (2013) Serial dilution via surface energy trap-assisted magnetic droplet manipulation. Lab Chip 13:4827–4831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Nicolini AM, Fronczek CF, Yoon J-Y (2015) Droplet-based immunoassay on a “sticky” nanofibrous surface for multiplexed and dual detection of bacteria using smartphones. Biosens Bioelectron 67:560–569

    Article  CAS  PubMed  Google Scholar 

  11. Park S-Y, Kalim S, Callahan C, Teitell MA, Chiou EPY (2009) A light-induced dielectrophoretic droplet manipulation platform. Lab Chip 9:3228–3235

    Article  CAS  PubMed  Google Scholar 

  12. He M, Edgar JS, Jeffries GDM, Lorenz RM, Shelby JP, Chiu DT (2005) Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Anal Chem 77:5214–5219

    Google Scholar 

  13. Huang CJ, Fang WF, Ke MS, Chou HYE, Yang JT (2014) A biocompatible open-surface droplet manipulation platform for detection of multi-nucleotide polymorphism. Lab Chip 14:2057–2062

    Article  CAS  PubMed  Google Scholar 

  14. Daniel S, Chaudhury MK (2005) Vibration-actuated drop motion on surfaces for batch microfluidic processes. Langmuir 4:4240–4248

    Article  Google Scholar 

  15. Darhuber AA, Valentino JP, Davis JM, Troian SM, Wagner S (2003) Microfluidic actuation by modulation of surface stresses. Appl Phys Lett 82:657

    Article  CAS  Google Scholar 

  16. Yoon J-Y, You DJ (2008) Backscattering particle immunoassays in wire-guide droplet manipulations. J Biol Eng 2:15

    Article  PubMed  PubMed Central  Google Scholar 

  17. You DJ, Tran PL, Kwon H-J, Patel D, Yoon J-Y (2011) Very quick reverse transcription polymerase chain reaction for detecting 2009 H1N1 influenza A using wire-guide droplet manipulations. Faraday Discuss 149:159–170

    Article  CAS  PubMed  Google Scholar 

  18. Harshman DK, Reyes R, Park TS, You DJ, Song J-Y, Yoon J-Y (2014) Enhanced nucleic acid amplification with blood in situ by wire-guided droplet manipulation (WDM). Biosens Bioelectron 53:167–174

    Article  CAS  PubMed  Google Scholar 

  19. de Gennes PG (1985) Wetting: statics and dynamics. Rev Mod Phys 57:827–863

    Article  Google Scholar 

  20. Song B, Springer J (1996) Determination of interfacial tension from the profile of a pendant drop using computer-aided image processing. J Colloid Interface Sci 184:64–76

    CAS  PubMed  Google Scholar 

  21. Tate T (1864) On the magnitude of a drop of liquid formed under different circumstances. Philos Mag Ser 4(181):176–180

    Google Scholar 

  22. Yoon J-Y, Garrell RL (2003) Preventing biomolecular adsorption in electrowetting-based biofluidic chips. Anal Chem 75:5097–5102

    Article  CAS  Google Scholar 

  23. Bartzoka V, Brook MA, McDermott MR (1998) Protein–silicone interactions: how compatible are the two species? Langmuir 14:1887–1891

    Article  CAS  Google Scholar 

  24. Tran PL, Gamboa JR, You DJ, Yoon J-Y (2010) FRET detection of Octamer-4 on a protein nanoarray made by size-dependent self-assembly. Anal Bioanal Chem 398:759–768

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51:263–273

    Article  CAS  PubMed  Google Scholar 

  26. Chien A, Edgar DB, Trela JM (1976) Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 127:1550–1557

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Brock T, Freeze H (1969) Thermus aquaticus gen. n. and sp. n., a non-sporulating extreme thermophile. J Bacteriol 98:289–297

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Zhang C, Xing D (2007) Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends. Nucleic Acids Res 35:4223–4237

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Roper MG, Easley CJ, Landers JP (2015) Advances in polymerase chain reaction on microfluidic chips. Anal Chem 77:3887–3893

    Article  Google Scholar 

  30. Farrar JS, Wittwer CT (2015) Extreme PCR: efficient and specific DNA amplification in 15–60 seconds. Clin Chem 61:145–153

    Article  PubMed  Google Scholar 

  31. Wittwer CT, Chris G, Garling J (1990) Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 331:328–331

    Article  Google Scholar 

  32. Innis MA, Myambo KB, Gelfand DH, Brow MAD (1988) DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc Natl Acad Sci U S A 85:9436–9440

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Wilson IG (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63:3741–3751

    CAS  PubMed  PubMed Central  Google Scholar 

  34. Rossen L, Norskov P, Hoimstrom K, Rasmussen OF (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol 17:37–45

    Article  CAS  PubMed  Google Scholar 

  35. Ra P, Al-soud WA, Jo LJ, Rådstro P (2001) Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39:485–493

    Article  Google Scholar 

  36. Katzman M (1993) Use of oil overlays in “oil-free” PCR technology. Biotechniques 14:36–40

    CAS  PubMed  Google Scholar 

  37. Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microbiol 62:1102–1106

    CAS  PubMed  PubMed Central  Google Scholar 

  38. Harshman DK, Rao BM, McLain JE, Watts GS, Yoon J-Y (2015) Innovative qPCR using interfacial effects to enable low threshold cycle detection and inhibition. Sci Adv 1:e1400061

    Article  PubMed  PubMed Central  Google Scholar 

  39. Becker A, Reith A, Kadenbach B (1996) A quantitative method of determining initial amounts of DNA by polymerase chain reaction cycle titration using digital imaging and a novel DNA stain. Anal Biochem 237:204–207

    Article  CAS  PubMed  Google Scholar 

  40. Higuchi R, Dollinger G, Walsh PS, Griffith R (1992) Simultaneous amplification and detection of specific DNA sequences. Nat Biotechnol 10:413–417

    Article  CAS  Google Scholar 

  41. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP (1997) Continuous fluorescent monitoring of rapid cycle DNA amplification. Biotechniques 22:130–138

    CAS  PubMed  Google Scholar 

  42. Gašparič MB, Tengs T, La Paz JL, Holst-Jensen A, Pla M, Esteve T, Žek J, Gruden K (2010) Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. Anal Bioanal Chem 396:2023–2029

    Article  Google Scholar 

  43. Tyagi S, Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14:303–308

    Article  CAS  PubMed  Google Scholar 

  44. Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6:986–994

    Article  CAS  PubMed  Google Scholar 

  45. Dragan AI, Pavlovic R, McGivney JB, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD (2012) SYBR Green I: fluorescence properties and interaction with DNA. J Fluoresc 22:1189–1199

    Article  CAS  PubMed  Google Scholar 

  46. Angus SV, Cho S, Harshman DK, Song J-Y, Yoon J-Y (2015) A portable, shock-proof, surface-heated droplet PCR system for Escherichia coli detection. Biosens Bioelectron 74:360–368

    Article  CAS  PubMed  Google Scholar 

  47. Rutledge RG, Côté C (2003) Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res 31:e93

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Biomedical Engineering Graduate Interdisciplinary Program and Department of Agricultural and Biosystems Engineering, The University of Arizona, 1177 East 4th Street, Room 403, Tucson, AZ, 85721-0038, USA

    Dustin K. Harshman Ph.D. & Jeong-Yeol Yoon Ph.D.

Authors
  1. Dustin K. Harshman Ph.D.
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  2. Jeong-Yeol Yoon Ph.D.
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Correspondence to Jeong-Yeol Yoon Ph.D. .

Editor information

Editors and Affiliations

  1. Virginia Tech, Blacksburg, Virginia, USA

    Chang Lu

  2. Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, Virginia, USA

    Scott S. Verbridge

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Harshman, D.K., Yoon, JY. (2016). Wire-guided Droplet Manipulation for Molecular Biology. In: Lu, C., Verbridge, S. (eds) Microfluidic Methods for Molecular Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-30019-1_12

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