Chapter

Biosensors for Security and Bioterrorism Applications

Part of the series Advanced Sciences and Technologies for Security Applications pp 443-468

Date:

Label-Free Optical Biosensors for Monitoring Cellular Processes and Cytotoxic Agents at Interfaces Using Guided Modes and Advanced Phase-Contrast Imaging Techniques

  • Inna SzékácsAffiliated withNanobiosensorics Group, Institute for Technical Physics and Material Science, Centre for Energy Research, Hungarian Academy of Sciences
  • , Robert HorvathAffiliated withNanobiosensorics Group, Institute for Technical Physics and Material Science, Centre for Energy Research, Hungarian Academy of Sciences
  • , András SzékácsAffiliated withAgro-Environmental Research Institute, National Agricultural Research and Innovation Centre Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Novel optical biosensors in direct interaction with living cells open new avenues in the detection of given cell types, including pathogenic microorganisms, and in the measurement of cytotoxicity of given xenobiotics, such as biothreat agents, on suitably selected cell types or lines. Evanescent optical field based biosensors emerging for such applications are reviewed, covering surface plasmon and waveguide based formats, imaging setup, employing optical waveguides, and digital holographic microscopy. Commercial technologies, along with main pathogenic microorganisms (Bacillus anthracis, Escherichia coli, Listeria monocytogenes, Campylobacter jejuni, etc.) as analytes and cell lines (native tissue and tumor cells) as cytotoxicity effect subjects are highlighted.

Keywords

Evanescent field Cell biosensor Optical waveguide Pathogenic microorganisms Cytotoxicity Digital holographic microscopy