International Conference on Medical Image Computing and Computer-Assisted Intervention

Medical Image Computing and Computer-Assisted Intervention -- MICCAI 2015 pp 661-668

Who Is Talking to Whom: Synaptic Partner Detection in Anisotropic Volumes of Insect Brain

  • Anna Kreshuk
  • Jan Funke
  • Albert Cardona
  • Fred A. Hamprecht
Conference paper

DOI: 10.1007/978-3-319-24553-9_81

Part of the Lecture Notes in Computer Science book series (LNCS, volume 9349)
Cite this paper as:
Kreshuk A., Funke J., Cardona A., Hamprecht F.A. (2015) Who Is Talking to Whom: Synaptic Partner Detection in Anisotropic Volumes of Insect Brain. In: Navab N., Hornegger J., Wells W., Frangi A. (eds) Medical Image Computing and Computer-Assisted Intervention -- MICCAI 2015. Lecture Notes in Computer Science, vol 9349. Springer, Cham

Abstract

Automated reconstruction of neural connectivity graphs from electron microscopy image stacks is an essential step towards large-scale neural circuit mapping. While significant progress has recently been made in automated segmentation of neurons and detection of synapses, the problem of synaptic partner assignment for polyadic (one-to-many) synapses, prevalent in the Drosophila brain, remains unsolved. In this contribution, we propose a method which automatically assigns pre- and postsynaptic roles to neurites adjacent to a synaptic site. The method constructs a probabilistic graphical model over potential synaptic partner pairs which includes factors to account for a high rate of one-to-many connections, as well as the possibility of the same neuron to be pre-synaptic in one synapse and post-synaptic in another. The algorithm has been validated on a publicly available stack of ssTEM images of Drosophila neural tissue and has been shown to reconstruct most of the synaptic relations correctly.

Keywords

Circuit reconstruction graphical model electron microscopy 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Anna Kreshuk
    • 1
    • 3
  • Jan Funke
    • 2
  • Albert Cardona
    • 3
  • Fred A. Hamprecht
    • 1
  1. 1.HCI/IWRUniversity of HeidelbergHeidelbergGermany
  2. 2.Institute of NeuroinformaticsUZH/ETH ZurichZurichSwitzerland
  3. 3.HHMI Janelia Research CampusAshburnUSA

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