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Visualizing Cortical Tissue Optical Changes During Seizure Activity with Optical Coherence Tomography

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Video Bioinformatics

Part of the book series: Computational Biology ((COBO,volume 22))

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Abstract

Optical coherence tomography (OCT) is a label-free, high resolution, minimally invasive imaging tool, which can produce millimeter depth-resolved cross-sectional images. We identified changes in the backscattered intensity of infrared light, which occurred during the development of induced seizures in vivo in mice. In a large region of interest, we observed significant decreases in the OCT intensity from cerebral cortex tissue preceding and during generalized tonic-clonic seizures induced with pentylenetetrazol (PTZ). We then leveraged the full spatiotemporal resolution of OCT by studying the temporal evolution of localized changes in backscattered intensity in three dimensions and analyzed the seizure propagation in time-resolved 3D functional images. This allowed for a better understanding and visualization of this biological phenomenon.

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Acknowledgments

This research was sponsored at UC Riverside by the National Institutes of Health R00-EB007241, K08-NS059674, and R01-NS081243; the National Science Foundation IGERT Video Bioinformatics DGE 0903667; and the UC Discovery Grant #213073.

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Authors and Affiliations

  1. Department of Bioengineering, Materials Science and Engineering 243, University of California, 900 University Ave., Riverside, CA, 92507, USA

    M. M. Eberle, C. L. Rodriguez, Y. Wang & B. H. Park

  2. Translational Neuroscience Laboratory, University of California, 1247 Webber Hall, 900 University Ave., Riverside, CA, 92507, USA

    J. I. Szu

  3. Translational Neuroscience Laboratory, University of California, 1234B Genomics, 900 University Ave., Riverside, CA, 92507, USA

    M. S. Hsu

  4. School of Medicine, University of California, 1247 Webber Hall, 900 University Ave., Riverside, CA, 92507, USA

    D. K. Binder

Authors
  1. M. M. Eberle
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  2. C. L. Rodriguez
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  3. J. I. Szu
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  4. Y. Wang
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  5. M. S. Hsu
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  6. D. K. Binder
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  7. B. H. Park
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Corresponding author

Correspondence to M. M. Eberle .

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Editors and Affiliations

  1. University of California, Riverside, California, USA

    Bir Bhanu

  2. University of California, Riverside, California, USA

    Prue Talbot

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Eberle, M.M. et al. (2015). Visualizing Cortical Tissue Optical Changes During Seizure Activity with Optical Coherence Tomography. In: Bhanu, B., Talbot, P. (eds) Video Bioinformatics. Computational Biology, vol 22. Springer, Cham. https://doi.org/10.1007/978-3-319-23724-4_6

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  • DOI: https://doi.org/10.1007/978-3-319-23724-4_6

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-23723-7

  • Online ISBN: 978-3-319-23724-4

  • eBook Packages: Computer ScienceComputer Science (R0)

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