Abstract
In the present study, relationship between cell orientation angle and strain value of membrane was comprehensively investigated using inhomogeneous strain field. And an axial strain threshold of cell, which corresponds to launch of cell orientation migration, was elucidated. One of the advantages in this study was that the inhomogeneous strain distribution was easily created by making a little improvement in a commonly-used uniaxial stretching device. The strains of two-dimensional stretched membrane were quantified position by position using digital image correlation (DIC) method. A 3D histogram of the cell frequency, which correlated with the cell orientation angle and normal strain of the membrane, made it possible to determine the axial strain threshold accurately. The value was 4.4 ± 0.3 %, which was reasonable compared with past study conducted by other researcher, although the past experiments were based on cyclic uniaxial stretch stimulation (homogeneous strain field). In addition, a preferential axial strain of the cell was achieved using the same technique of the determination of the axial strain threshold. This work has novel values at three points: (i) Determining axial strain threshold of the cells precisely. (ii) First suggestion of preferential axial strain of the cells. (iii) Investigating methodically cell behavior in inhomogeneous strain field.
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References
G. Giannone, M.P. Sheetz, Substrate rigidity and force define form through tyrosine phosphatase and kinase pathways. Trends Cell Biol. 16, 213–223 (2006)
S.L.Y. Woo, M.A. Gomez, Y.K. Woo, W.H. Akeson, Mechanical-properties of tendons and ligaments II. The relationships of immobilization and exercise on tissue remodeling. Biorheology 19, 397–408 (1982)
Y. Morita, S. Watanabe, Y. Ju, B. Xu, Determination of optimal cyclic uniaxial stretches for stem cell-to-tenocyte differentiation under a wide range of mechanical stretch conditions by evaluating gene expression and protein synthesis levels. Acta Bioeng. Biomech. 15, 71–79 (2013)
C. Neidlinger-Wilke, E.S. Grood, J.H.C. Wang, R.A. Brand, L. Claes, Cell alignment is induced by cyclic changes in cell length: studies of cells grown in cyclically stretched substrates. J. Orthop. Res. 19, 286–293 (2001)
H.C. Wang, W. Ip, R. Boissy, E.S. Grood, Cell orientation response to cyclically deformed substrates: experimental validation of a cell model. J. Biomech. 28, 1543–1552 (1995)
L. Zhang, C.J.F. Kahn, H.Q. Chen, N. Tran, X. Wang, Effect of uniaxial stretching on rat bone mesenchymal stem cell: orientation and expressions of collagen types I and III and tenascin-C. Cell Biol. Int. 32, 344–352 (2008)
S.P. Arold, J.Y. Wong, B. Suki, Design of a new stretching apparatus and the effects of cyclic strain and substratum on mouse lung epithelial-12 cells. Ann. Biomed. Eng. 35, 1156–1164 (2007)
G.P. Raeber, M.P. Lutolf, J.A. Hubbell, Part II: fibroblasts preferentially migrate in the direction of principal strain. Biomech. Model. Mechan. 7, 215–225 (2008)
G.G. Yang, R.C. Crawford, J.H.C. Wang, Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions. J. Biomech. 37, 1543–1550 (2004)
B. Xu, G. Song, Y. Ju, Effect of focal adhesion kinase on the regulation of realignment and tenogenic differentiation of human mesenchymal stem cells by mechanical stretch. Connect. Tissue Res. 52, 373–379 (2011)
B. Xu, G. Song, Y. Ju, X. Li, Y. Song, S. Watanabe, RhoA/ROCK, cytoskeletal dynamics and focal adhesion kinase are required for mechanical stretch-induced tenogenic differentiation of human mesenchymal stem cells. J. Cell. Physiol. 227, 2722–2729 (2012)
L. Zhang, N. Tran, H.Q. Chen, C.J.F. Kahn, S. Marchal, F. Groubatch, X. Wang, Time-related changes in expression of collagen types I and III and of tenascin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching. Cell Tissue Res. 332, 101–109 (2008)
R.C. Buck, Reorientation response of cells to repeated stretch and recoil of the substratum. Exp. Cell Res. 127, 470–474 (1980)
M.J. Buckley, A.J. Banes, L.G. Levin, B.E. Sumpio, M. Sato, R. Jordan, J. Gilbert, G.W. Link, R.T.S. Tay, Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro. Bone Miner. 4, 225–236 (1988)
P.C. Dartsch, H. Hammerle, Orientation response of arterial smooth muscle cells to mechanical stimulation. Eur. J. Cell Biol. 41, 339–346 (1986)
M.A. Sutton, Digital image correlation for shape and deformation measurements, in Springer handbook of experimental solid mechanics, ed. by W.N. Sharpe (Springer, New York, 2008), pp. 565–600
Y. Morita, M. Uchino, M. Todo, Y. Matsushita, K. Arakawa, K. Koyano, Relationship between load-displacement curve and deformation distribution in porcine mandibular periodontium. J. Biomech. Sci. Eng. 4, 336–344 (2009)
Y. Morita, Y. Matsushita, M. Todo, K. Koyano, Experimental study on displacement and strain distributions of bone model with dental implant. Appl. Mech. Mater. 83, 73–77 (2011)
H. Inoh, N. Ishiguro, S.I. Sawazaki, H. Amma, M. Miyazu, H. Iwata, M. Sokabe, K. Naruse, Uni-axial cyclic stretch induces the activation of transcription factor nuclear factor B in human fibroblast cells. FASEB J. 16, 405 (2002)
Y.C. Yung, H. Vandenburgh, D.J. Mooney, Cellular strain assessment tool (CSAT): precision-controlled cyclic uniaxial tensile loading. J. Biomech. 42, 178–182 (2009)
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Morita, Y., Sato, T., Watanabe, S., Ju, Y. (2016). In Vitro Experimental Study for the Determination of Cellular Characteristics of Mesenchymal Stem Cells Using a Non-uniform Deformation Field. In: Sciammarella, C., Considine, J., Gloeckner, P. (eds) Experimental and Applied Mechanics, Volume 4. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-22449-7_16
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DOI: https://doi.org/10.1007/978-3-319-22449-7_16
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