Abstract
The term “pre-analytics” summarizes all procedures concerned with specimen collection or processing as well as logistical aspects like transport or storage of tissue specimens. All or these variables as well as tissue-specific characteristics affect sample quality. While certain parameters like warm ischemia or tissue-specific characteristics cannot be changed, other parameters can be assessed and optimized. The aim of this study was to determine RNA quality by assessing the RIN values of specimens from different organs and to assess the influence of vacuum preservation. Samples from the GI tract, in general, appear to have lower RNA quality when compared to samples from other organ sites. This may be due to the digestive enzymes or bacterial colonization. Processing time in pathology does not significantly influence RNA quality. Tissue preservation with a vacuum sealer leads to preserved RNA quality over an extended period of time and offers a feasible alternative to minimize the influence of transport time into pathology.
This manuscript contains data adapted from SZ’s master thesis: ‘Qualitätssicherung von Tumor- und Normalgewebebanken am Beispiel des Universitätskrebszentrums Dresden’. University Hospital Carl Gustav Carus, Faculty of Medicine, Dresden, Germany.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Brimo F, Aprikian A, Latour M et al (2013) Strategies for biochemical and pathologic quality assurance in a large multi-institutional biorepository; the experience of the PROCURE Quebec Prostate Cancer Biobank. Biopreserv Biobank 11:285–290
Bussolati G, Chiusa L, Cimino A et al (2008) Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin. Virchows Archiv Int J Pathol 452:229–231
Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 27:126–139
Hwang RF, Wang H, Lara A et al (2008) Development of an integrated biospecimen bank and multidisciplinary clinical database for pancreatic cancer. Ann Surg Oncol 15:1356–1366
Mcdonald SA (2010) Principles of research tissue banking and specimen evaluation from the pathologist’s perspective. Biopreserv Biobank 8:197–201
Mcdonald SA, Chernock RD, Leach TA et al (2011) Procurement of human tissues for research banking in the surgical pathology laboratory: prioritization practices at Washington University Medical Center. Biopreserv Biobank 9:245–251
Muley TR, Herth FJ, Schnabel PA et al (2012) From tissue to molecular phenotyping: pre-analytical requirements. Transl Lung Cancer Res 1:111–121
Ortiz J, Siddeswarappa M, Sea S et al (2011) The elimination of warm ischemic time in kidney transplantation using the ice bag technique: a feasibility study. J Exp Clin Med 3:187–190
Rudloff U, Bhanot U, Gerald W et al (2010) Biobanking of human pancreas cancer tissue: impact of ex-vivo procurement times on RNA quality. Ann Surg Oncol 17:2229–2236
Salehi Z, Najafi M (2014) RNA preservation and stabilization. Biochem Physiol Open Access 3:126
Schroeder A, Mueller O, Stocker S et al (2006) The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 7:3
Tang W, Hu Z, Muallem H et al (2011) Clinical implementation of RNA signatures for pharmacogenomic decision-making. Pharmacogenomics Personal Med 4:95–107
Tang W, Hu Z, Muallem H et al (2012) Quality assurance of RNA expression profiling in clinical laboratories. J Mol Diagn 14:1–11
Viana C, Neto C, Kerr L et al (2013) The interference of cold ischemia time in the quality of total RNA from frozen tumor samples. Cell Tissue Bank 14:167–173
Yildiz-Aktas IZ, Dabbs DJ, Bhargava R (2012) The effect of cold ischemic time on the immunohistochemical evaluation of estrogen receptor, progesterone receptor, and HER2 expression in invasive breast carcinoma. Mod Pathol an official journal of the United States and Canadian Academy of Pathology, Inc 25:1098–1105
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Zeugner, S., Mayr, T., Zietz, C., Aust, D.E., Baretton, G.B. (2015). RNA Quality in Fresh-Frozen Gastrointestinal Tumor Specimens—Experiences from the Tumor and Healthy Tissue Bank TU Dresden. In: Dietel, M., Wittekind, C., Bussolati, G., von Winterfeld, M. (eds) Pre-Analytics of Pathological Specimens in Oncology. Recent Results in Cancer Research, vol 199. Springer, Cham. https://doi.org/10.1007/978-3-319-13957-9_9
Download citation
DOI: https://doi.org/10.1007/978-3-319-13957-9_9
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-13956-2
Online ISBN: 978-3-319-13957-9
eBook Packages: MedicineMedicine (R0)