Chapter

Ultrafast Nonlinear Optics

Part of the series Scottish Graduate Series pp 251-286

Date:

Utilising Ultrafast Lasers for Multiphoton Biomedical Imaging

  • Erich E. HooverAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines Email author 
  • , Eric V. ChandlerAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines
  • , Jeffrey J. FieldAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines
  • , Dawn N. VitekAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines
  • , Michael D. YoungAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines
  • , Jeff A. SquierAffiliated withCenter for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics, Colorado School of Mines Email author 

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Abstract

This chapter covers the benefits and applications of ultrafast laser scanning microscopes from a biomedical perspective. The basic architecture of a laser microscope is discussed, including how to design a laser scanning system with lateral and axial control. Also investigated is the design of custom collection optics for optimizing the detection of emitted photons and maximizing that emitted fluorescence in the presence of photobleaching. In addition, this chapter addresses three techniques novel to the biomedical community. The first is the technique of temporal focusing and its application toward wide-field imaging and micromachining. Also investigated is the concept of photon counting in multiphoton microscopy and how this approach to imaging has become practical for everyday use. Finally, several different methods are revealed for implementing spectral imaging with a multiphoton microscope platform.