Abstract
Multiplex in-situ hybridization (M-FISH) is a combinatorial labeling technique in which each chromosome is labeled with 5 fluors and a DNA stain and is used for chromosome analysis. Although M-FISH facilitates the visual detection of gross anomalies, misclassified pixels and cross-hybridization often makes manual examination difficult and introduces operator bias. The success of the technique largely depends on the accuracy of pixel classification. In this work we study the use of nonlinear Vector Median Filtering (VMF) methods to induce the accuracy of pixel classification. We have evaluated our methodology using a subset of images publicly available and the classifier was trained and tested on non-overlapping chromosome images. An overall accuracy of 74.13% is reported when introducing VMF.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Thompson, M., McInnes, R., Willard, H.: Genetics in Medicine. 5th Edition, WB Saunders Company, (1991) Philadelphia.
Speicher, M.R., Ballard, S.G., Ward, D.C.: Karyotyping human chromosomes by combinatorial Multi-Fluor FISH. Nat. Genetics, 12. (1996) 341–344.
Wang, Y.P., Wu, Q., Castleman, K.R., Xiong, Z.: Chromosome image enhancement using multiscale differential operators. IEEE Trans. Med. Imag. 22 (2003) 685–693.
Veldman, T., Vignon, C., Schröck, E., Rowley, J.D., Ried, T.: Hidden chromosome abnormalities in hematological malignancies detected by multicolor spectral karyotyping. Nat. Genetics. 15 (1997) 406–410.
Castleman, K.R., Morrison, E., Piper, J., Saracoglu, K., Schultz, M., Speicher, M.R.: Classification accuracy in multiple color fluorescence imaging microscopy. Cytometry. 41 (2000) 139–147.
Wang, Y., Castleman, K.R.: Normalization of multicolor fluorescence in situ hybridization (M-FISH) images for improving color karyotyping. Cytometry. 64 (2005) 101–109.
Sampat, M.P., Castleman, K.R., Bovik, A.C.: Pixel-by-Pixel classification of MFISH images. In Proc. 24th Annual International Conference of the IEEE EMBS. (2002) 999–1000.
Schwartzkopf, W.C., Bovik, A.C., Evans, B.L.: Maximum-likelihood techniques for joint segmentation-classification of multispectral chromosome images. IEEE Trans. Med. Imag. 24 (2005) 1593–1610.
Sampat, M.P., Bovik, A.C., Aggarwal, J.K., Castleman, K.R.: Supervised parametric and non-parametric classification of chromosome images. Pattern Recognit. 38 (2005) 1209–1223.
Karvelis, P.S., Fotiadis, D.I., Georgiou, I., Syrrou, M.: A watershed based segmentation method for multispectral chromosome image classification. In Proc. 28th Annual International Conference of the IEEE EMBS. (2006) 3009–3012.
Astola, J., Haavisto, P., Neuov, Y.: Vector median filter. Proc. IEEE. 78 (1990) 678–689.
Trahanias, P.E., Venetsanopoulos, A.N.: Vector directional filters—a new class of multichannel image processing filters. IEEE Trans. Image Process. 2 (1993) 528–534.
Trahanias, P.E., Karakos, D. Venetsanopoulos A.N.: Directional processing of color images: Theory and experimental results. IEEE Trans. Image Process. 5 (1996) 868–881.
Viero, T., Oistamo, K., Neuvo, Y.: Three-dimensional median related filters for color image sequence filtering. IEEE Trans. Circ. Syst. Video Technol. 4 (1994) 129–142.
Alparone, L. Barni, M., Bartolini, F., Caldelli, R.: Regularization of optic flow estimates by means of weighted vector median filtering. IEEE Trans. Image Process. 8 (1999) 1462–1467.
Lukac, R.: Adaptive color image filtering based on center-weighted vector directional filters. Multidimensional Syst. Signal Process. 15 (2004) 169–196.
Lukac, R., Smolka, B., Plataniotis, K.N., Venetsanopoulos, A.N.: Selection weighted vector directional filter. Comput. Vis. Image Understand. 94 (2004) 140–167. The ADIR M_FISH Image Database. Available at: http://www.adires.com/05/Project/MFISH_DB/MFISH_DB.shtml.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer-Verlag London Limited
About this paper
Cite this paper
Karvelis, P.S., Fotiadis, D.I. (2007). Enhancement of Multispectral Chromosome Image Classification Using Vector Median Filtering. In: Akhgar, B. (eds) ICCS 2007. Springer, London. https://doi.org/10.1007/978-1-84628-992-7_7
Download citation
DOI: https://doi.org/10.1007/978-1-84628-992-7_7
Publisher Name: Springer, London
Print ISBN: 978-1-84628-990-3
Online ISBN: 978-1-84628-992-7
eBook Packages: Computer ScienceComputer Science (R0)